Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)

This study describes the identification of the Plasmodium vivax rhoptry antigen Pv34 whose sequence was obtained based on homology comparison with the Plasmodium falciparum Pf34. The pv34 gene product was characterized by molecular biology and immunological techniques. Additionally, association of P...

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Detalles Bibliográficos
Autores: Mongui, Alvaro, Angel, Diana I., Gallego, Gina, Reyes, Claudia, Martinez, Paola, Guhl, Felipe, Patarroyo, Manuel A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Colombia
Institución:Universidad del Rosario
Repositorio:Repositorio EdocUR - U. Rosario
Idioma:inglés
OAI Identifier:oai:repository.urosario.edu.co:10336/23457
Acceso en línea:https://doi.org/10.1016/j.vaccine.2009.10.034
https://repository.urosario.edu.co/handle/10336/23457
Access Level:acceso abierto
Palabra clave:Chloroquine
Gamma interferon
Interleukin 10
Interleukin 2
Interleukin 4
Interleukin 5
Malaria vaccine
Primaquine
Recombinant plasmodium vivax 34 antigen
Recombinant protein
Tumor necrosis factor alpha
Unclassified drug
Animal experiment
Article
Blood sampling
Cytokine release
Drug antigenicity
Human
Humoral immunity
Immunogenicity
Lymphocyte proliferation
Molecular biology
Monkey
Nonhuman
Nucleotide sequence
Peripheral blood mononuclear cell
Plasmodium vivax
Plasmodium vivax malaria
Priority journal
Protein blood level
Rabbit
Adult
Aged
Animals
Cytokines
Haplorhini
Humans
Malaria vaccines
Middle aged
Protozoan proteins
Rabbits
Pf34 homologue
Vaccine candidate
indirect
polyacrylamide gel
western
Blotting
Electrophoresis
Fluorescent antibody technique
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spelling Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)Mongui, AlvaroAngel, Diana I.Gallego, GinaReyes, ClaudiaMartinez, PaolaGuhl, FelipePatarroyo, Manuel A.ChloroquineGamma interferonInterleukin 10Interleukin 2Interleukin 4Interleukin 5Malaria vaccinePrimaquineRecombinant plasmodium vivax 34 antigenRecombinant proteinTumor necrosis factor alphaUnclassified drugAnimal experimentArticleBlood samplingCytokine releaseDrug antigenicityHumanHumoral immunityImmunogenicityLymphocyte proliferationMolecular biologyMonkeyNonhumanNucleotide sequencePeripheral blood mononuclear cellPlasmodium vivaxPlasmodium vivax malariaPriority journalProtein blood levelRabbitAdultAgedAnimalsCytokinesHaplorhiniHumansMalaria vaccinesMiddle agedPlasmodium vivaxProtozoan proteinsRabbitsPf34 homologuePlasmodium vivaxVaccine candidateindirectpolyacrylamide gelwesternBlottingElectrophoresisFluorescent antibody techniqueThis study describes the identification of the Plasmodium vivax rhoptry antigen Pv34 whose sequence was obtained based on homology comparison with the Plasmodium falciparum Pf34. The pv34 gene product was characterized by molecular biology and immunological techniques. Additionally, association of Pv34 to detergent-resistant microdomains (DRMs), expression in late blood-stage parasites and recognition of recombinant Pv34 (rPv34) by sera from P. vivax-infected Aotus monkeys and patients was assessed. Lymphoproliferation and cytokine secretion was also evaluated in individuals living in malaria endemic areas. Altogether, the data support carrying out further studies to assess the immunogenicity and protection-inducing ability of rPv34 as component of a multi-antigenic, multi-stage vaccine against vivax malaria. © 2009 Elsevier Ltd. All rights reserved.20092020-05-26T00:02:11Zinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.vaccine.2009.10.0340264410X13588745https://repository.urosario.edu.co/handle/10336/23457reponame:Repositorio EdocUR - U. Rosarioinstname:Universidad del Rosarioinstacron:Universidad del Rosarioenghttps://www.scopus.com/inward/record.uri?eid=2-s2.0-70649093849&doi=10.1016%2fj.vaccine.2009.10.034&partnerID=40&md5=a74ebb3c1ffe22e37c9bd7bf88dba4c0info:eu-repo/semantics/openAccess2022-05-02T07:37:20Z
dc.title.none.fl_str_mv Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)
title Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)
spellingShingle Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)
Mongui, Alvaro
Chloroquine
Gamma interferon
Interleukin 10
Interleukin 2
Interleukin 4
Interleukin 5
Malaria vaccine
Primaquine
Recombinant plasmodium vivax 34 antigen
Recombinant protein
Tumor necrosis factor alpha
Unclassified drug
Animal experiment
Article
Blood sampling
Cytokine release
Drug antigenicity
Human
Humoral immunity
Immunogenicity
Lymphocyte proliferation
Molecular biology
Monkey
Nonhuman
Nucleotide sequence
Peripheral blood mononuclear cell
Plasmodium vivax
Plasmodium vivax malaria
Priority journal
Protein blood level
Rabbit
Adult
Aged
Animals
Cytokines
Haplorhini
Humans
Malaria vaccines
Middle aged
Plasmodium vivax
Protozoan proteins
Rabbits
Pf34 homologue
Plasmodium vivax
Vaccine candidate
indirect
polyacrylamide gel
western
Blotting
Electrophoresis
Fluorescent antibody technique
title_short Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)
title_full Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)
title_fullStr Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)
title_full_unstemmed Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)
title_sort Characterization and antigenicity of the promising vaccine candidate Plasmodium vivax 34 kDa rhoptry antigen (Pv34)
dc.creator.none.fl_str_mv Mongui, Alvaro
Angel, Diana I.
Gallego, Gina
Reyes, Claudia
Martinez, Paola
Guhl, Felipe
Patarroyo, Manuel A.
author Mongui, Alvaro
author_facet Mongui, Alvaro
Angel, Diana I.
Gallego, Gina
Reyes, Claudia
Martinez, Paola
Guhl, Felipe
Patarroyo, Manuel A.
author_role author
author2 Angel, Diana I.
Gallego, Gina
Reyes, Claudia
Martinez, Paola
Guhl, Felipe
Patarroyo, Manuel A.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Chloroquine
Gamma interferon
Interleukin 10
Interleukin 2
Interleukin 4
Interleukin 5
Malaria vaccine
Primaquine
Recombinant plasmodium vivax 34 antigen
Recombinant protein
Tumor necrosis factor alpha
Unclassified drug
Animal experiment
Article
Blood sampling
Cytokine release
Drug antigenicity
Human
Humoral immunity
Immunogenicity
Lymphocyte proliferation
Molecular biology
Monkey
Nonhuman
Nucleotide sequence
Peripheral blood mononuclear cell
Plasmodium vivax
Plasmodium vivax malaria
Priority journal
Protein blood level
Rabbit
Adult
Aged
Animals
Cytokines
Haplorhini
Humans
Malaria vaccines
Middle aged
Plasmodium vivax
Protozoan proteins
Rabbits
Pf34 homologue
Plasmodium vivax
Vaccine candidate
indirect
polyacrylamide gel
western
Blotting
Electrophoresis
Fluorescent antibody technique
topic Chloroquine
Gamma interferon
Interleukin 10
Interleukin 2
Interleukin 4
Interleukin 5
Malaria vaccine
Primaquine
Recombinant plasmodium vivax 34 antigen
Recombinant protein
Tumor necrosis factor alpha
Unclassified drug
Animal experiment
Article
Blood sampling
Cytokine release
Drug antigenicity
Human
Humoral immunity
Immunogenicity
Lymphocyte proliferation
Molecular biology
Monkey
Nonhuman
Nucleotide sequence
Peripheral blood mononuclear cell
Plasmodium vivax
Plasmodium vivax malaria
Priority journal
Protein blood level
Rabbit
Adult
Aged
Animals
Cytokines
Haplorhini
Humans
Malaria vaccines
Middle aged
Plasmodium vivax
Protozoan proteins
Rabbits
Pf34 homologue
Plasmodium vivax
Vaccine candidate
indirect
polyacrylamide gel
western
Blotting
Electrophoresis
Fluorescent antibody technique
description This study describes the identification of the Plasmodium vivax rhoptry antigen Pv34 whose sequence was obtained based on homology comparison with the Plasmodium falciparum Pf34. The pv34 gene product was characterized by molecular biology and immunological techniques. Additionally, association of Pv34 to detergent-resistant microdomains (DRMs), expression in late blood-stage parasites and recognition of recombinant Pv34 (rPv34) by sera from P. vivax-infected Aotus monkeys and patients was assessed. Lymphoproliferation and cytokine secretion was also evaluated in individuals living in malaria endemic areas. Altogether, the data support carrying out further studies to assess the immunogenicity and protection-inducing ability of rPv34 as component of a multi-antigenic, multi-stage vaccine against vivax malaria. © 2009 Elsevier Ltd. All rights reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009
2020-05-26T00:02:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.vaccine.2009.10.034
0264410X
13588745
https://repository.urosario.edu.co/handle/10336/23457
url https://doi.org/10.1016/j.vaccine.2009.10.034
https://repository.urosario.edu.co/handle/10336/23457
identifier_str_mv 0264410X
13588745
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-70649093849&doi=10.1016%2fj.vaccine.2009.10.034&partnerID=40&md5=a74ebb3c1ffe22e37c9bd7bf88dba4c0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositorio EdocUR - U. Rosario
instname:Universidad del Rosario
instacron:Universidad del Rosario
instname_str Universidad del Rosario
instacron_str Universidad del Rosario
institution Universidad del Rosario
reponame_str Repositorio EdocUR - U. Rosario
collection Repositorio EdocUR - U. Rosario
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score 15,812455

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