Wound healing activity of Ullucus tuberosus, an Andean tuber crop

ABSTRACT: This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus (U. tuberosus) using in vitro models. Lyophilized pulp and acetone extracts of U. tuberosus were produced using ultrasound extraction. The capacity for collagenase activation was eval...

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Detalles Bibliográficos
Autores: Osorio Durango, Edinson, Bravo Muñoz, Karent Elizabeth, Montoya, Andrés, Robledo, Sara María, Heil, Nathalie
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Colombia
Institución:Universidad de Antioquia
Repositorio:Repositorio UdeA
Idioma:inglés
OAI Identifier:oai:bibliotecadigital.udea.edu.co:10495/20242
Acceso en línea:http://hdl.handle.net/10495/20242
Access Level:acceso abierto
Palabra clave:Cicatrización de Heridas
Wound Healing
Colagenasa Microbiana
Microbial Collagenase
Metaloproteinasa 1 de la Matriz
Matrix Metalloproteinase 1
Ullucus tuberosus
http://aims.fao.org/aos/agrovoc/c_8041
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spelling Wound healing activity of Ullucus tuberosus, an Andean tuber cropOsorio Durango, EdinsonBravo Muñoz, Karent ElizabethMontoya, AndrésRobledo, Sara MaríaHeil, NathalieCicatrización de HeridasWound HealingColagenasa MicrobianaMicrobial CollagenaseMetaloproteinasa 1 de la MatrizMatrix Metalloproteinase 1Ullucus tuberosushttp://aims.fao.org/aos/agrovoc/c_8041ABSTRACT: This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus (U. tuberosus) using in vitro models. Lyophilized pulp and acetone extracts of U. tuberosus were produced using ultrasound extraction. The capacity for collagenase activation was evaluated using fluorescence detection of the enzymatic activity. Then, the influence of U. tuberosus extracts on cell proliferation, cell migration and synthesis of the extracellular matrix (ECM) proteins, metalloproteinase (MMP-1) and pro-collagen was analyzed using human dermal fibroblasts in culture. An increase in collagenase activity of 12% supports the utility of U. tuberosus as an agent for scar treatment. In addition, the extracts showed an increase in the proliferation and migration of human dermal fibroblasts and the production of pro-collagen and MMP-1 after treatment with U. tuberosus extracts. The increase in proliferation, migration and pro-collagen levels positively influenced the regeneration of scarless tissue during the proliferation phase, whereas the increase in MMP-1 may have favored the wound healing process during the remodeling and cellular differentiation phases. The results of this study show for first time that U. tuberosus is a promising candidate to support scarless tissue regeneration.COL0010359COL0015099Asian Pacific Tropical Medicine PressGrupo de Investigación en Sustancias Bioactivas (GISB)Programa de Estudio y Control de Enfermedades Tropicales (PECET)Haikou, China2021-06-22T19:32:00Z2021-06-22T19:32:00Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigación6application/pdfapplication/pdf2221-1691http://hdl.handle.net/10495/2024210.1016/j.apjtb.2017.05.007engAsian Pac J Trop Biomedinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/reponame:Repositorio UdeAinstname:Universidad de Antioquiainstacron:Universidad de Antioquia2021-06-22T19:32:01Z
dc.title.none.fl_str_mv Wound healing activity of Ullucus tuberosus, an Andean tuber crop
title Wound healing activity of Ullucus tuberosus, an Andean tuber crop
spellingShingle Wound healing activity of Ullucus tuberosus, an Andean tuber crop
Osorio Durango, Edinson
Cicatrización de Heridas
Wound Healing
Colagenasa Microbiana
Microbial Collagenase
Metaloproteinasa 1 de la Matriz
Matrix Metalloproteinase 1
Ullucus tuberosus
http://aims.fao.org/aos/agrovoc/c_8041
title_short Wound healing activity of Ullucus tuberosus, an Andean tuber crop
title_full Wound healing activity of Ullucus tuberosus, an Andean tuber crop
title_fullStr Wound healing activity of Ullucus tuberosus, an Andean tuber crop
title_full_unstemmed Wound healing activity of Ullucus tuberosus, an Andean tuber crop
title_sort Wound healing activity of Ullucus tuberosus, an Andean tuber crop
dc.creator.none.fl_str_mv Osorio Durango, Edinson
Bravo Muñoz, Karent Elizabeth
Montoya, Andrés
Robledo, Sara María
Heil, Nathalie
author Osorio Durango, Edinson
author_facet Osorio Durango, Edinson
Bravo Muñoz, Karent Elizabeth
Montoya, Andrés
Robledo, Sara María
Heil, Nathalie
author_role author
author2 Bravo Muñoz, Karent Elizabeth
Montoya, Andrés
Robledo, Sara María
Heil, Nathalie
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cicatrización de Heridas
Wound Healing
Colagenasa Microbiana
Microbial Collagenase
Metaloproteinasa 1 de la Matriz
Matrix Metalloproteinase 1
Ullucus tuberosus
http://aims.fao.org/aos/agrovoc/c_8041
topic Cicatrización de Heridas
Wound Healing
Colagenasa Microbiana
Microbial Collagenase
Metaloproteinasa 1 de la Matriz
Matrix Metalloproteinase 1
Ullucus tuberosus
http://aims.fao.org/aos/agrovoc/c_8041
description ABSTRACT: This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus (U. tuberosus) using in vitro models. Lyophilized pulp and acetone extracts of U. tuberosus were produced using ultrasound extraction. The capacity for collagenase activation was evaluated using fluorescence detection of the enzymatic activity. Then, the influence of U. tuberosus extracts on cell proliferation, cell migration and synthesis of the extracellular matrix (ECM) proteins, metalloproteinase (MMP-1) and pro-collagen was analyzed using human dermal fibroblasts in culture. An increase in collagenase activity of 12% supports the utility of U. tuberosus as an agent for scar treatment. In addition, the extracts showed an increase in the proliferation and migration of human dermal fibroblasts and the production of pro-collagen and MMP-1 after treatment with U. tuberosus extracts. The increase in proliferation, migration and pro-collagen levels positively influenced the regeneration of scarless tissue during the proliferation phase, whereas the increase in MMP-1 may have favored the wound healing process during the remodeling and cellular differentiation phases. The results of this study show for first time that U. tuberosus is a promising candidate to support scarless tissue regeneration.
publishDate 2017
dc.date.none.fl_str_mv 2017
2021-06-22T19:32:00Z
2021-06-22T19:32:00Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_2df8fbb1
https://purl.org/redcol/resource_type/ART
Artículo de investigación
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 2221-1691
http://hdl.handle.net/10495/20242
10.1016/j.apjtb.2017.05.007
identifier_str_mv 2221-1691
10.1016/j.apjtb.2017.05.007
url http://hdl.handle.net/10495/20242
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Asian Pac J Trop Biomed
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv 6
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asian Pacific Tropical Medicine Press
Grupo de Investigación en Sustancias Bioactivas (GISB)
Programa de Estudio y Control de Enfermedades Tropicales (PECET)
Haikou, China
publisher.none.fl_str_mv Asian Pacific Tropical Medicine Press
Grupo de Investigación en Sustancias Bioactivas (GISB)
Programa de Estudio y Control de Enfermedades Tropicales (PECET)
Haikou, China
dc.source.none.fl_str_mv reponame:Repositorio UdeA
instname:Universidad de Antioquia
instacron:Universidad de Antioquia
instname_str Universidad de Antioquia
instacron_str Universidad de Antioquia
institution Universidad de Antioquia
reponame_str Repositorio UdeA
collection Repositorio UdeA
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score 15,81155

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