Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
ABSTRACT: In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the reg...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | Colombia |
| Institución: | Universidad de Antioquia |
| Repositorio: | Repositorio UdeA |
| Idioma: | inglés |
| OAI Identifier: | oai:bibliotecadigital.udea.edu.co:10495/20379 |
| Acceso en línea: | http://hdl.handle.net/10495/20379 |
| Access Level: | acceso abierto |
| Palabra clave: | Antioxidants Antioxidantes Histology Histología Plant Somatic Embryogenesis Techniques Técnicas de Embriogénesis Somática de Plantas Banco de semillas del suelo Soil seed banks Embriogénesis somática Somatic embryogenesis Recalcitrancia Recalcitrance http://aims.fao.org/aos/agrovoc/c_35298 http://aims.fao.org/aos/agrovoc/c_36911 |
| Sumario: | ABSTRACT: In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating variety had a high presence of polysaccharides with random distribution of polyphenols at the end of the embryo induction step. |
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