ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO

The importance of obtaining the different stages of Trypanosoma cruzi in order to recognize the antigens involved in the intracellular invasion and replication processes, makes it necessary to adapt these strains to tissue culture, especially considering the high leve! of biological, biochemical and...

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Detalles Bibliográficos
Autores: Velazco Gamboa, Carolina; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Puentes Corredor, Manuel; Instituto de Inmunología - HSJD, Bogotá, Moreno García, Alberto; Instituto de Inmunología - HSJD, Bogotá, Patarroyo Murillo, Manuel Elkin; Instituto de Inmunología - HSJD, Bogotá, Puerta Bula, Concepción; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Colombia
Institución:Pontificia Universidad Javeriana
Repositorio:Repositorio Universidad Javeriana
Idioma:inglés
OAI Identifier:oai:repository.javeriana.edu.co:10554/31443
Acceso en línea:http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5074
http://hdl.handle.net/10554/31443
Access Level:acceso abierto
Palabra clave:null
Cultivo tisular; tripomastigotes; amastigotes; epimastigotes; Trypanosoma cruzi
Descripción
Sumario:The importance of obtaining the different stages of Trypanosoma cruzi in order to recognize the antigens involved in the intracellular invasion and replication processes, makes it necessary to adapt these strains to tissue culture, especially considering the high leve! of biological, biochemical and genetic variation, which is found among the strains and clones ofthe parasite. Within the aforementioned context, in this study, the Colombian strain ofT. cruzi, M un anta, was adapted to tissue culture in Yero Cells, obtaining the principal forms ofthe parasite: trypomastigotes, amastigotes and epimastigotes. lt was observed that the liberation of the trypomastigotes occurred on the seventh day postinfection and the quantity obtained was directly proportional to the number of infecting parasites. On the other hand, the majority of the trypomastigotes observed 48 hours after washing the monolayer five days after infection, were had thick-looking shapes corresponding to the initiation ofthe trasforrnation from trypomastigotes to amastigotes.

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