In vitro genotoxic effect of secondary minerals crystallized in rocks from coal mine drainage

Coal processing generates a large volume of waste that can damage human health and the environment. Often these wastes produce acid drainage in which several minerals are crystallized (evaporites). This study aimed to identify secondary minerals, as well as the genotoxic potential of these materials...

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Detalles Bibliográficos
Autores: Perachi Nordin, Adriane, Da Silva, Juliana, Telles De Souza, Claudia, Niekraszewiczl Boufleur, Liana Appe, Ferraz Dias, Johnny, Da Boit Martinello, Katia, Silva Oliveira, Marcos Leandro, Grivicich, Ivana, Garcia Hilario, Ana Leticia, Silva Oliveira, Luis Felipe, Da Silva Rabaioli, Fernanda
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:Colombia
Institución:Corporación Universidad de la Costa
Repositorio:Repositorio REDICUC
Idioma:inglés
OAI Identifier:oai:repositorio.cuc.edu.co:11323/1573
Acceso en línea:https://hdl.handle.net/11323/1573
https://doi.org/10.1016/j.jhazmat.2017.12.026
https://repositorio.cuc.edu.co/
Access Level:acceso abierto
Palabra clave:Coal Mine Drainage
Comet Assay
Evaporite
Micronucleus Test
System Biology
Descripción
Sumario:Coal processing generates a large volume of waste that can damage human health and the environment. Often these wastes produce acid drainage in which several minerals are crystallized (evaporites). This study aimed to identify secondary minerals, as well as the genotoxic potential of these materials. The samples were collected at two sites along the Rocinha River in Santa Catarina state (Brazil): (1) directly from the source of the acid drainage (evaporite 1), and (2) on the river bank (evaporite 2). The samples were characterized by X-ray diffraction and by particle-induced X-ray emission techniques. In vitro genotoxicity testing using Comet assay and Micronucleus test in V79 cells was used to evaluate evaporite samples. Our study also used System Biology tools to provide insight regarding the influence of this exposure on DNA damage in cells. The results showed that the samples induced DNA damage for both evaporites that can be explained by high concentrations of chromium, iron, nickel, copper and zinc in these materials. Thus, this study is very important due to the dearth of knowledge regarding the toxicity of evaporites in the environment. The genetic toxicity of this material can be induced by increased oxidative stress and DNA repair inhibition.

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