Proteomic profile of human monocytic cells infected with dengue virus

Objective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with...

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Autores: Martínez-Betancur, V., Martínez Gutiérrez, Marlén
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Colombia
Institución:Universidad Cooperativa de Colombia
Repositorio:Repositorio UCC
OAI Identifier:oai:repository.ucc.edu.co:20.500.12494/49550
Acceso en línea:https://doi.org/10.1016/j.apjtb.2016.01.004
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958212695&doi=10.1016%2fj.apjtb.2016.01.004&partnerID=40&md5=f0568aa6a770118bff896dcb6dbed4db
https://hdl.handle.net/20.500.12494/49550
Access Level:acceso abierto
Palabra clave:ARTICLE
BETA TUBULIN
CELL PROTEIN
CELL VIABILITY
CONTROLLED STUDY
DENGUE VIRUS 2
DRUG TARGETING
ENOLASE
FATTY ACID BINDING PROTEIN
HEAT SHOCK PROTEIN
HEAT SHOCK PROTEIN 90
HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN
HUMAN
HUMAN CELL
LIPOCORTIN 4
MASS SPECTROMETRY
MONOCYTE
NONHUMAN
PATHOGENICITY
PHOSPHOLIPASE C
PRIORITY JOURNAL
PROTEIN ANALYSIS
PROTEIN DISULFIDE ISOMERASE
PROTEIN EXPRESSION
PROTEOME
PROTEOMICS
PYRUVATE KINASE
TRANSALDOLASE
TWO DIMENSIONAL ELECTROPHORESIS
U937 CELL LINE
VIRAL PROTEIN
VIRUS DNA
VIRUS PARTICLE
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oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/49550
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repository_id_str
spelling Proteomic profile of human monocytic cells infected with dengue virusMartínez-Betancur, V.Martínez Gutiérrez, MarlénARTICLEBETA TUBULINCELL PROTEINCELL VIABILITYCONTROLLED STUDYDENGUE VIRUS 2DRUG TARGETINGENOLASEFATTY ACID BINDING PROTEINHEAT SHOCK PROTEINHEAT SHOCK PROTEIN 90HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINHUMANHUMAN CELLLIPOCORTIN 4MASS SPECTROMETRYMONOCYTENONHUMANPATHOGENICITYPHOSPHOLIPASE CPRIORITY JOURNALPROTEIN ANALYSISPROTEIN DISULFIDE ISOMERASEPROTEIN EXPRESSIONPROTEOMEPROTEOMICSPYRUVATE KINASETRANSALDOLASETWO DIMENSIONAL ELECTROPHORESISU937 CELL LINEVIRAL PROTEINVIRUS DNAVIRUS PARTICLEObjective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein quantity. Results In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially (five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed (two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 kDa protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 kDa protein AA1, tubulin beta, enolase 1, pyruvate kinase, transaldolase and phospholipase C-alpha. Conclusions Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection. © 2016 Hainan Medical UniversityHainan Medical University2023-05-24T16:21:30Z2023-05-24T16:21:30Z01/01/2016Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersion914-923https://doi.org/10.1016/j.apjtb.2016.01.004https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958212695&doi=10.1016%2fj.apjtb.2016.01.004&partnerID=40&md5=f0568aa6a770118bff896dcb6dbed4db22211691https://hdl.handle.net/20.500.12494/49550Martínez-Betancur V.,Martinez Gutierrez Marlen.Proteomic profile of human monocytic cells infected with dengue virus.Asian Pacific Journal of Tropical Biomedicine. 2016. 6. (11):p. 914-923Asian Pacific Journal of Tropical Biomedicineinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2reponame:Repositorio UCCinstname:Universidad Cooperativa de Colombiainstacron:Universidad Cooperativa de Colombia2024-08-20T21:23:24Z
dc.title.none.fl_str_mv Proteomic profile of human monocytic cells infected with dengue virus
title Proteomic profile of human monocytic cells infected with dengue virus
spellingShingle Proteomic profile of human monocytic cells infected with dengue virus
Martínez-Betancur, V.
ARTICLE
BETA TUBULIN
CELL PROTEIN
CELL VIABILITY
CONTROLLED STUDY
DENGUE VIRUS 2
DRUG TARGETING
ENOLASE
FATTY ACID BINDING PROTEIN
HEAT SHOCK PROTEIN
HEAT SHOCK PROTEIN 90
HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN
HUMAN
HUMAN CELL
LIPOCORTIN 4
MASS SPECTROMETRY
MONOCYTE
NONHUMAN
PATHOGENICITY
PHOSPHOLIPASE C
PRIORITY JOURNAL
PROTEIN ANALYSIS
PROTEIN DISULFIDE ISOMERASE
PROTEIN EXPRESSION
PROTEOME
PROTEOMICS
PYRUVATE KINASE
TRANSALDOLASE
TWO DIMENSIONAL ELECTROPHORESIS
U937 CELL LINE
VIRAL PROTEIN
VIRUS DNA
VIRUS PARTICLE
title_short Proteomic profile of human monocytic cells infected with dengue virus
title_full Proteomic profile of human monocytic cells infected with dengue virus
title_fullStr Proteomic profile of human monocytic cells infected with dengue virus
title_full_unstemmed Proteomic profile of human monocytic cells infected with dengue virus
title_sort Proteomic profile of human monocytic cells infected with dengue virus
dc.creator.none.fl_str_mv Martínez-Betancur, V.
Martínez Gutiérrez, Marlén
author Martínez-Betancur, V.
author_facet Martínez-Betancur, V.
Martínez Gutiérrez, Marlén
author_role author
author2 Martínez Gutiérrez, Marlén
author2_role author
dc.subject.none.fl_str_mv ARTICLE
BETA TUBULIN
CELL PROTEIN
CELL VIABILITY
CONTROLLED STUDY
DENGUE VIRUS 2
DRUG TARGETING
ENOLASE
FATTY ACID BINDING PROTEIN
HEAT SHOCK PROTEIN
HEAT SHOCK PROTEIN 90
HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN
HUMAN
HUMAN CELL
LIPOCORTIN 4
MASS SPECTROMETRY
MONOCYTE
NONHUMAN
PATHOGENICITY
PHOSPHOLIPASE C
PRIORITY JOURNAL
PROTEIN ANALYSIS
PROTEIN DISULFIDE ISOMERASE
PROTEIN EXPRESSION
PROTEOME
PROTEOMICS
PYRUVATE KINASE
TRANSALDOLASE
TWO DIMENSIONAL ELECTROPHORESIS
U937 CELL LINE
VIRAL PROTEIN
VIRUS DNA
VIRUS PARTICLE
topic ARTICLE
BETA TUBULIN
CELL PROTEIN
CELL VIABILITY
CONTROLLED STUDY
DENGUE VIRUS 2
DRUG TARGETING
ENOLASE
FATTY ACID BINDING PROTEIN
HEAT SHOCK PROTEIN
HEAT SHOCK PROTEIN 90
HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN
HUMAN
HUMAN CELL
LIPOCORTIN 4
MASS SPECTROMETRY
MONOCYTE
NONHUMAN
PATHOGENICITY
PHOSPHOLIPASE C
PRIORITY JOURNAL
PROTEIN ANALYSIS
PROTEIN DISULFIDE ISOMERASE
PROTEIN EXPRESSION
PROTEOME
PROTEOMICS
PYRUVATE KINASE
TRANSALDOLASE
TWO DIMENSIONAL ELECTROPHORESIS
U937 CELL LINE
VIRAL PROTEIN
VIRUS DNA
VIRUS PARTICLE
description Objective To identify the changes in the proteome of U937 cells infected with dengue virus (DENV). Methods In this study, differentiated U937 cultures were infected with two DENV-2 strains, one of which was associated with dengue (DENV-2/NG) and the other one with severe dengue (DENV-2/16681), with the aim of determining the cellular proteomic profiles under different infection conditions. Cellular proteins were extracted and separated by two-dimensional electrophoresis, and those proteins with differential expression profiles were identified by mass spectrometry. The obtained results were correlated with cellular viability, the number of infectious viral particles, and the viral DNA/protein quantity. Results In comparison with non-infected cultures, in the cells infected with the DENV-2/NG strain, nine proteins were expressed differentially (five were upregulated and four were downregulated); in those cultures infected with the DENV-2/16681 strain, six proteins were differentially expressed (two were downregulated and four were upregulated). The downregulated proteins included fatty acid-binding protein, heterogeneous nuclear ribonucleoprotein 1, protein disulfide isomerase, enolase 1, heat shock 70 kDa protein 9, phosphotyrosyl phosphatase, and annexin IV. The upregulated proteins included heat shock 90 kDa protein AA1, tubulin beta, enolase 1, pyruvate kinase, transaldolase and phospholipase C-alpha. Conclusions Because the monocyte/macrophage lineage is critical for disease pathogenicity, additional studies on these proteins could provide a better understanding of the cellular response to DENV infection and could help identify new therapeutic targets against infection. © 2016 Hainan Medical University
publishDate 2023
dc.date.none.fl_str_mv 01/01/2016
2023-05-24T16:21:30Z
2023-05-24T16:21:30Z
dc.type.none.fl_str_mv Artículo
http://purl.org/coar/resource_type/c_6501
http://purl.org/coar/version/c_970fb48d4fbd8a85
info:eu-repo/semantics/article
http://purl.org/redcol/resource_type/ART
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.apjtb.2016.01.004
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958212695&doi=10.1016%2fj.apjtb.2016.01.004&partnerID=40&md5=f0568aa6a770118bff896dcb6dbed4db
22211691
https://hdl.handle.net/20.500.12494/49550
Martínez-Betancur V.,Martinez Gutierrez Marlen.Proteomic profile of human monocytic cells infected with dengue virus.Asian Pacific Journal of Tropical Biomedicine. 2016. 6. (11):p. 914-923
url https://doi.org/10.1016/j.apjtb.2016.01.004
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958212695&doi=10.1016%2fj.apjtb.2016.01.004&partnerID=40&md5=f0568aa6a770118bff896dcb6dbed4db
https://hdl.handle.net/20.500.12494/49550
identifier_str_mv 22211691
Martínez-Betancur V.,Martinez Gutierrez Marlen.Proteomic profile of human monocytic cells infected with dengue virus.Asian Pacific Journal of Tropical Biomedicine. 2016. 6. (11):p. 914-923
dc.relation.none.fl_str_mv Asian Pacific Journal of Tropical Biomedicine
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf2
dc.format.none.fl_str_mv 914-923
dc.publisher.none.fl_str_mv Hainan Medical University
publisher.none.fl_str_mv Hainan Medical University
dc.source.none.fl_str_mv reponame:Repositorio UCC
instname:Universidad Cooperativa de Colombia
instacron:Universidad Cooperativa de Colombia
instname_str Universidad Cooperativa de Colombia
instacron_str Universidad Cooperativa de Colombia
institution Universidad Cooperativa de Colombia
reponame_str Repositorio UCC
collection Repositorio UCC
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score 15,812455

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