Analysis of gene expression profiles related to phenol degradation in the environmental strain Scedosporium apiospermum HDO1
Filamentous fungi are efficient microorganisms in the degradation of petroleum hydrocarbons. Among them Scedosporium apiospermum has been reported as able to degrade phenol, a pollutant widely produced in industrial waste. Two initial phenol degradation pathways have been proposed for the fungal spe...
| Autor: | |
|---|---|
| Tipo de documento: | dissertação |
| Estado: | Versión aceptada para publicación |
| Data de publicação: | 2018 |
| País: | Colombia |
| Recursos: | Universidad de los Andes |
| Repositório: | Séneca: repositorio Uniandes |
| Idioma: | inglês |
| OAI Identifier: | oai:repositorio.uniandes.edu.co:1992/73899 |
| Acesso em linha: | https://hdl.handle.net/1992/73899 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Phenol Scedosporium apiospermum Bioremediation Catechol Hydroquinone Biología |
| Resumo: | Filamentous fungi are efficient microorganisms in the degradation of petroleum hydrocarbons. Among them Scedosporium apiospermum has been reported as able to degrade phenol, a pollutant widely produced in industrial waste. Two initial phenol degradation pathways have been proposed for the fungal species: one via catechol and the other through hydroquinone. Based on genomic information, genes related to the latter two pathways coding for phenol hydroxylase and catechol 1,2-dioxygenase have been reported in the environmental strain S.apiospermum HDO1. The present study assessed the transcriptional changes of the genes involved in the phenol degradation pathways proposed in S.apiospermum HDO1. The fungal strain was grown during four days in Salts Minimum Medium under two carbon sources, phenol or glucose (control). The mycelium grown was collected and sent for RNA extraction and then sequencing with RNA-seq (Illumina Hiseq-4000 platform). For the five enzymes previously reported for the ortho-catechol pathway, we identified four genes codifying for phenol 2-monooxygenase, three genes for Catechol dioxygenase, and one gene codifying for hydroxyquinol 1,2-dioxygenase. Several genes involved in the first steps of the pollutant transformation were differentially expressed, these results suggest that the degradation of phenol by S.apiospermum is achieved via the ortho-catechol pathway. |
|---|