Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis

We compared the efficiency of three methods of agarose and polyacrylamide electrophoresis (using the small tank of the DNA Sequencing System and the large OWL Sequi-Gen Sequencing Cell), for the detection of polymorphism in 21 accessions of orange (Citrus sinensis), using the primer CGA. The polyacr...

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Autores: Morillo Coronado, Ana Cruz, Chagüeza Villarreal, Yamilet, Caicedo Arana, Alvaro, Jaramillo Vásquez, Juan, Muñoz Rivera, Oscar Julian, Arcos, Alba Lucía, Vásquez Amariles, Herney Darío, Muñoz Flores, Jaime Eduardo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Colombia
Institución:Universidad Nacional de Colombia
Repositorio:Repositorio UN
Idioma:español
OAI Identifier:oai:repositorio.unal.edu.co:unal/48017
Acceso en línea:https://repositorio.unal.edu.co/handle/unal/48017
http://bdigital.unal.edu.co/41374/
Access Level:acceso abierto
Palabra clave:Citrus sinensis
electroforesis
agarosa
poliacrilamida
RAMs
electrophoresis
agarose
polyacrylamide
RAMs.
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spelling Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresisMorillo Coronado, Ana CruzChagüeza Villarreal, YamiletCaicedo Arana, AlvaroJaramillo Vásquez, JuanMuñoz Rivera, Oscar JulianArcos, Alba LucíaVásquez Amariles, Herney DaríoMuñoz Flores, Jaime EduardoCitrus sinensiselectroforesisagarosapoliacrilamidaRAMselectrophoresisagarosepolyacrylamideCitrus sinensiselectrophoresisagarosepolyacrylamideRAMs.We compared the efficiency of three methods of agarose and polyacrylamide electrophoresis (using the small tank of the DNA Sequencing System and the large OWL Sequi-Gen Sequencing Cell), for the detection of polymorphism in 21 accessions of orange (Citrus sinensis), using the primer CGA. The polyacrylamide gel gave better resolution of the PCR-amplified RAM products. This method allowed better detection of polymorphic DNA bands, facilitating the identification of genetic variability. The agarose electrophoresis may be more convenient in other applications, due to its low cost and easy implementation. The study of genetic diversity in orange using RAMs separated 51 accessions into seven groups with 0.75 similarity, and 0.25 heterozygosity, revealing low genetic polymorphism. The RAMs technique grouped the accessions into “Common or White”, “Navel” and “Pigmented or “Sanguine”.Se compararon las eficiencias de tres métodos de electroforesis en agarosa y poliacrilamida, usando la cámara pequeña de DNA Sequencing System y cámara grande OWL Sequi-Gen Sequencing Cell, en la detección del polimorfismo en 21 accesiones de naranja (Citrus sinensis) con empleo del cebador CGA. El gel de poliacrilamida dio mejor resolución de los productos amplificados vía PCR producidos por RAMs. Este permitió una mejor detección de bandas de ADN polimórficas, lo que facilitó la identificación de la variabilidad genética. La electroforesis en agarosa puede ser más conveniente en otras aplicaciones, debido al bajo costo y fácil aplicación. El estudio de diversidad genética en naranja usando microsatélites RAMs diferenció 51 accesiones en siete grupos con 0.75 de similaridad y 0.25 de heterocigosidad, lo que revela bajo polimorfismo genético. La técnica RAMs permitió agrupar las accesiones en Comunes o Blancas, Navel y Pigmentadas o Sanguinas.Universidad Nacional de Colombia Sede Palmira2019-06-29T07:34:07Z2019-06-29T07:34:07Z2009Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTapplication/mswordtext/htmlapplication/mswordapplication/pdfapplication/pdfhttps://repositorio.unal.edu.co/handle/unal/48017http://bdigital.unal.edu.co/41374/spahttp://revistas.unal.edu.co/index.php/acta_agronomica/article/view/12450Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaMorillo Coronado, Ana Cruz and Chagüeza Villarreal, Yamilet and Caicedo Arana, Alvaro and Jaramillo Vásquez, Juan and Muñoz Rivera, Oscar Julian and Arcos, Alba Lucía and Vásquez Amariles, Herney Darío and Muñoz Flores, Jaime Eduardo (2009) Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis. Acta Agronómica; Vol. 58, núm. 4 (2009); 234-244 Acta Agronómica; Vol. 58, núm. 4 (2009); 234-244 Acta Agronómica; Vol. 58, núm. 4 (2009); 234-244 2323-0118 0120-2812 .Derechos reservados - Universidad Nacional de ColombiaAtribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessreponame:Repositorio UNinstname:Universidad Nacional de Colombiainstacron:Universidad Nacional de Colombia2023-11-02T04:05:50Z
dc.title.none.fl_str_mv Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis
title Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis
spellingShingle Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis
Morillo Coronado, Ana Cruz
Citrus sinensis
electroforesis
agarosa
poliacrilamida
RAMs
electrophoresis
agarose
polyacrylamide
Citrus sinensis
electrophoresis
agarose
polyacrylamide
RAMs.
title_short Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis
title_full Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis
title_fullStr Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis
title_full_unstemmed Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis
title_sort Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis
dc.creator.none.fl_str_mv Morillo Coronado, Ana Cruz
Chagüeza Villarreal, Yamilet
Caicedo Arana, Alvaro
Jaramillo Vásquez, Juan
Muñoz Rivera, Oscar Julian
Arcos, Alba Lucía
Vásquez Amariles, Herney Darío
Muñoz Flores, Jaime Eduardo
author Morillo Coronado, Ana Cruz
author_facet Morillo Coronado, Ana Cruz
Chagüeza Villarreal, Yamilet
Caicedo Arana, Alvaro
Jaramillo Vásquez, Juan
Muñoz Rivera, Oscar Julian
Arcos, Alba Lucía
Vásquez Amariles, Herney Darío
Muñoz Flores, Jaime Eduardo
author_role author
author2 Chagüeza Villarreal, Yamilet
Caicedo Arana, Alvaro
Jaramillo Vásquez, Juan
Muñoz Rivera, Oscar Julian
Arcos, Alba Lucía
Vásquez Amariles, Herney Darío
Muñoz Flores, Jaime Eduardo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Citrus sinensis
electroforesis
agarosa
poliacrilamida
RAMs
electrophoresis
agarose
polyacrylamide
Citrus sinensis
electrophoresis
agarose
polyacrylamide
RAMs.
topic Citrus sinensis
electroforesis
agarosa
poliacrilamida
RAMs
electrophoresis
agarose
polyacrylamide
Citrus sinensis
electrophoresis
agarose
polyacrylamide
RAMs.
description We compared the efficiency of three methods of agarose and polyacrylamide electrophoresis (using the small tank of the DNA Sequencing System and the large OWL Sequi-Gen Sequencing Cell), for the detection of polymorphism in 21 accessions of orange (Citrus sinensis), using the primer CGA. The polyacrylamide gel gave better resolution of the PCR-amplified RAM products. This method allowed better detection of polymorphic DNA bands, facilitating the identification of genetic variability. The agarose electrophoresis may be more convenient in other applications, due to its low cost and easy implementation. The study of genetic diversity in orange using RAMs separated 51 accessions into seven groups with 0.75 similarity, and 0.25 heterozygosity, revealing low genetic polymorphism. The RAMs technique grouped the accessions into “Common or White”, “Navel” and “Pigmented or “Sanguine”.
publishDate 2009
dc.date.none.fl_str_mv 2009
2019-06-29T07:34:07Z
2019-06-29T07:34:07Z
dc.type.none.fl_str_mv Artículo de revista
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
http://purl.org/coar/version/c_970fb48d4fbd8a85
Text
http://purl.org/redcol/resource_type/ART
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/48017
http://bdigital.unal.edu.co/41374/
url https://repositorio.unal.edu.co/handle/unal/48017
http://bdigital.unal.edu.co/41374/
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://revistas.unal.edu.co/index.php/acta_agronomica/article/view/12450
Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica
Acta Agronómica
Morillo Coronado, Ana Cruz and Chagüeza Villarreal, Yamilet and Caicedo Arana, Alvaro and Jaramillo Vásquez, Juan and Muñoz Rivera, Oscar Julian and Arcos, Alba Lucía and Vásquez Amariles, Herney Darío and Muñoz Flores, Jaime Eduardo (2009) Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (rams), using polyacrylamide and agarose electrophoresis. Acta Agronómica; Vol. 58, núm. 4 (2009); 234-244 Acta Agronómica; Vol. 58, núm. 4 (2009); 234-244 Acta Agronómica; Vol. 58, núm. 4 (2009); 234-244 2323-0118 0120-2812 .
dc.rights.none.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos reservados - Universidad Nacional de Colombia
Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/msword
text/html
application/msword
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional de Colombia Sede Palmira
publisher.none.fl_str_mv Universidad Nacional de Colombia Sede Palmira
dc.source.none.fl_str_mv reponame:Repositorio UN
instname:Universidad Nacional de Colombia
instacron:Universidad Nacional de Colombia
instname_str Universidad Nacional de Colombia
instacron_str Universidad Nacional de Colombia
institution Universidad Nacional de Colombia
reponame_str Repositorio UN
collection Repositorio UN
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score 15,811543

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