Characterization of chitin and chitosan extracted from shrimp shells by two methods
ABSTRACT:Shrimp shells from Penaeus Vannamei species were hydrolyzed for chitin extraction by a chemical and a papain enzymatic method. Composition of shells was analyzed and their microstructure was characterized before and after hydrolysis by microscopy. Chitin fibers arrangement in the tissue was...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | Colombia |
| Institución: | Universidad de Antioquia |
| Repositorio: | Repositorio UdeA |
| Idioma: | inglés |
| OAI Identifier: | oai:bibliotecadigital.udea.edu.co:10495/30854 |
| Acceso en línea: | https://hdl.handle.net/10495/30854 https://www.degruyter.com/document/doi/10.1515/epoly.2010.10.1.748/html |
| Access Level: | acceso abierto |
| Palabra clave: | Quitina Chitin Hidrólisis Hydrolysis Papaína Papain Shell microstructure |
| Sumario: | ABSTRACT:Shrimp shells from Penaeus Vannamei species were hydrolyzed for chitin extraction by a chemical and a papain enzymatic method. Composition of shells was analyzed and their microstructure was characterized before and after hydrolysis by microscopy. Chitin fibers arrangement in the tissue was preserved after chemical extraction, but after papain hydrolysis the tissue presented structural disarrangement indicating that papain reacts indistinctly with peptidic and N-acetyl linkages. Although chemical purification is very effective, by-products are not recoverable. Conversely, papain hydrolysis yields partially purified chitosan but permits aminoacids isolation, which is important in food industry. This method has other advantages such as low cost and easy accessibility of papain. Chitin and chitosan were characterized by thermogravimetric analysis, infrared spectrophotometry and capillary electrophoresis. Degree of N-acetylation (DA) was determined by cross-polarization magic angle spinning nuclear magnetic resonance (CPMAS 13CNMR) or potentiometry and crystallinity was measured by X ray diffraction. |
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