The eukaryotic genome, its reads, and the unfinished assembly
In recent years, readily affordable short read sequences provided by next-generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest in the utility of NGS-only approaches for exploring eukaryotic genomes. The concept of a static, 'finished' genome...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Colombia |
| Institución: | Universidad del Rosario |
| Repositorio: | Repositorio EdocUR - U. Rosario |
| Idioma: | inglés |
| OAI Identifier: | oai:repository.urosario.edu.co:10336/24056 |
| Acceso en línea: | https://doi.org/10.1016/j.febslet.2013.05.048 https://repository.urosario.edu.co/handle/10336/24056 |
| Access Level: | acceso abierto |
| Palabra clave: | Accuracy Algorithm Article Bioinformatics Fungal genome Fungal strain Genome analysis Human Information processing Information retrieval Next generation sequencing Nonhuman Priority journal Reliability Reproducibility Sequence analysis Animals Base sequence Chromosome mapping Eukaryota Genomics High-throughput nucleotide sequencing Humans Molecular sequence annotation Assembly-free genome analysis Eukaryotic genomics Microbial strain collection Object-view separation dna human Genome |
| Sumario: | In recent years, readily affordable short read sequences provided by next-generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest in the utility of NGS-only approaches for exploring eukaryotic genomes. The concept of a static, 'finished' genome assembly, which still appears to be a faraway goal for many eukaryotes, is yielding to new paradigms. We here motivate an object-view concept where the raw reads are the main, fixed object, and assemblies with their annotations take a role of dynamically changing and modifiable views of that object. © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
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