Extraction and partial characterization of venom from the Colombian spider Pamphobeteus aff. nigricolor (Aranae:Theraphosidae)
We report the first studies of characterization and extraction of the Pamphobeteus aff. nigricolor (Pocock, 1901) (Aranae:Theraphosidae) venom done in Colombia using the electro-stimulation technique previous anesthesia with isofluorane. After each extraction process, a low viscosity, colorless veno...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | Colombia |
| Institución: | Universidad Cooperativa de Colombia |
| Repositorio: | Repositorio UCC |
| OAI Identifier: | oai:repository.ucc.edu.co:20.500.12494/49720 |
| Acceso en línea: | https://doi.org/10.1016/j.toxicon.2013.10.014 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84887689821&doi=10.1016%2fj.toxicon.2013.10.014&partnerID=40&md5=34e75b1e6440e80b01c819b136320eba https://hdl.handle.net/20.500.12494/49720 |
| Access Level: | acceso abierto |
| Palabra clave: | ANIMAL TISSUE ANIMALS ARANEAE ARTICLE BLOOD CLOTTING COLOMBIA CHEMICAL FRACTIONATION CHROMATOGRAPHY, HIGH PRESSURE LIQUID CHROMATOGRAPHY, REVERSE-PHASE DRY WEIGHT ELECTROPHORESIS ELECTROPHORESIS, POLYACRYLAMIDE GEL ELECTROSPRAY MASS SPECTROMETRY FEMALE HEMOLYSIS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY HUMIDITY MALE NONHUMAN PAMPHOBETEUS NIGRICOLOR PEPTIDE ANALYSIS PH MEASUREMENT PHOSPHOLIPASE A2 PRIORITY JOURNAL PROTEIN DEGRADATION REVERSED PHASE LIQUID CHROMATOGRAPHY RP-HPLC SEX FACTORS SPIDER VENOM SPIDER VENOMS SPIDERS TARANTULA THERAPHOSIDAE THERAPHOTOXIN PN1A THERAPHOTOXIN PN1B THERAPHOTOXIN PN2A TOXIN TOXIN ANALYSIS UNCLASSIFIED DRUG VENOM EXTRACTION VISCOSITY VOLTAGE GATED CALCIUM CHANNEL |
| Sumario: | We report the first studies of characterization and extraction of the Pamphobeteus aff. nigricolor (Pocock, 1901) (Aranae:Theraphosidae) venom done in Colombia using the electro-stimulation technique previous anesthesia with isofluorane. After each extraction process, a low viscosity, colorless venom was obtained. This venom showed a 1.01 mg/µl density and a pH of 5. The humidity percentage did not show a significance difference between males and females (P > 0.05) with a general media of 77.49 ± 1.74%. In all cases the venom yielded was variable between males and females, with a media of 22.45 ± 5.17 mg (wet weight) and 4.58 ± 0.94 mg (dry weigh), obtaining larger amounts in females, 28.34 ± 7.49 mg and 5.69 ± 1.36 (wet and dry weight respectively). Venom showed a hemolytic activity dependent of enzymatic active phospholipase and neither coagulant nor proteolytic activities were observed. Electrophoretic profile showed a main protein content with a molecular mass below 14 kDa. RP-HPLC venom profile revealed a difference among male and female venom's content where 17 and 21 main fractions were obtained respectively. Three peptides, Theraphotoxin-Pn1a, Theraphotoxin-Pn1b and Theraphotoxin-Pn2a, were identified using HPLC-nESI-MS/MS. These peptides showed a high identity with other peptides found on Theraphosides which are proved to affect voltage-gated calcium channels. © 2013 Elsevier Ltd. All rights reserved. |
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