Dissecting the functional roles of the conserved nxxe and hxe motifs of the adp-dependent glucokinase from thermococcus litoralis

The activity of the ADP-dependent glucokinase from Thermococcus litoralis (TIGK) relies on the highly conserved motifs NXXE (i.e. Asn-Xaa-Xaa-Glu) and HXE (i.e. His-Xaa-Glu). Site-directed mutagenesis of residues Glu279 (11XE) and Glu308 (NXXE) leads to e

Detalhes bibliográficos
Autores: Jose Abarca-Lagunas, Maria, Andres Rivas-Pardo, Jaime, Ramirez-Sarmiento, Cesar A, Guixe, Victoria
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2015
País:Chile
Idioma:inglês
OAI Identifier:oai:repositorio.anid.cl:10533/232059
Acesso em linha:https://hdl.handle.net/10533/232059
Access Level:Acceso aberto
Descrição
Resumo:The activity of the ADP-dependent glucokinase from Thermococcus litoralis (TIGK) relies on the highly conserved motifs NXXE (i.e. Asn-Xaa-Xaa-Glu) and HXE (i.e. His-Xaa-Glu). Site-directed mutagenesis of residues Glu279 (11XE) and Glu308 (NXXE) leads to e