Hantavirus Gn and Gc Glycoproteins Self-Assemble into Virus-Like Particles

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral...

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Detalles Bibliográficos
Autores: Acuña-Astudillo, Rodrigo Antonio, Cifuentes-Muñoz, Nicolás, Marquez, Chantal L, Bulling, Manuela, Klingstrom, Jonas, Mancini, Roberta, Lozach, Pierre-Yves, Tischler-Dworschak, Nicole
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/220777
Acceso en línea:https://hdl.handle.net/10533/220777
Access Level:acceso abierto
Descripción
Sumario:How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera. Keywords. KeyWords Plus:KOREAN HEMORRHAGIC-FEVER; SIN-NOMBRE-VIRUS; HANTAAN-VIRUS; CYTOPLASMIC TAIL; UUKUNIEMI-VIRUS; DNA VACCINE; NUCLEOCAPSID PROTEIN; ELECTRON-MICROSCOPY; PULMONARY SYNDROME; NONHUMAN-PRIMATES