“IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”

Pathogenicity islands excision is a mechanism that occurs in several Enterobacteriaceae family members, including Salmonella enterica subspecie enterica serovars. ROD21 is an excisable pathogenicity island present in the chromosome of Salmonella enterica serovar Enteritidis and other serovars. It ha...

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Autor: Pardo Roa, Catalina
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2018
País:Chile
OAI Identifier:oai:repositorio.anid.cl:10533/232956
Acceso en línea:https://hdl.handle.net/10533/232956
Access Level:acceso abierto
Palabra clave:Ciencias Naturales
Otras Ciencias Naturales
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dc.title.es_CL.fl_str_mv “IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”
title “IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”
spellingShingle “IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”
Pardo Roa, Catalina
Ciencias Naturales
Otras Ciencias Naturales
title_short “IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”
title_full “IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”
title_fullStr “IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”
title_full_unstemmed “IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”
title_sort “IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”
dc.creator.none.fl_str_mv Pardo Roa, Catalina
author Pardo Roa, Catalina
author_facet Pardo Roa, Catalina
author_role author
dc.contributor.advisor.none.fl_str_mv Bueno, Susan M
dc.contributor.institution.es_CL.fl_str_mv PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE
dc.subject.oecd1n.es_CL.fl_str_mv Ciencias Naturales
topic Ciencias Naturales
Otras Ciencias Naturales
dc.subject.oecd2n.es_CL.fl_str_mv Otras Ciencias Naturales
description Pathogenicity islands excision is a mechanism that occurs in several Enterobacteriaceae family members, including Salmonella enterica subspecie enterica serovars. ROD21 is an excisable pathogenicity island present in the chromosome of Salmonella enterica serovar Enteritidis and other serovars. It has been described that excision of ROD21 may play an important role in the ability of S. Enteritidis to cause systemic infection in the murine model, because strain unable to excise ROD21 showed a reduced ability to colonize the liver and the spleen. In this work, the kinetics of ROD21 excision in vivo across the stages of the infective cycle performed by S. Enteritidis in C57BL/6 mice was evaluated and the importance of ROD21 excision on Salmonella infection ability and gene expression was determined. After different times post infection, bacterial load and excision frequency was quantified in different portions of the digestive tract and internal organs. It was observed that the frequency of ROD21 excision increased significantly in the bacterial population able to colonize mesenteric lymph nodes at early stages, remaining very low in liver and spleen during these stages. However, a drastic increase in ROD21 excision was observed at late times post infection when intestinal re-infection and mortality start to happen. These observations suggest that the inability to excise ROD21 affects the translocation of S. Enteritidis from the intestine to the mesenteric lymph nodes due, possibly, to a defect in its capacity to invade host cells. On the other hand, the assays showed that impairment to excise ROD21 can affect the expression of genes located both within and outside the island. These results may be indicating that ROD21´s excision is a genetic mechanism employed by S. Enteritidis to regulate the expression of its virulence genes, to adapt to the conditions found in different tissues and to produce a successful systemic infection in the mouse.
publishDate 2018
dc.date.issued.es_CL.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2019-02-13T19:38:22Z
2022-08-16T18:44:59Z
dc.date.available.none.fl_str_mv 2019-02-13T19:38:22Z
2022-08-16T18:44:59Z
dc.date.embargo.es_CL.fl_str_mv info:eu-repo/date/embargoEnd/2020-01-01
dc.type.none.fl_str_mv Tesis Magíster
dc.type.driver.es_CL.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.tesis.none.fl_str_mv Tesis
format doctoralThesis
status_str publishedVersion
dc.identifier.folio.es_CL.fl_str_mv 21140169
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/232956
identifier_str_mv 21140169
url https://hdl.handle.net/10533/232956
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.projectid.es_CL.fl_str_mv info:eu-repo/grantAgreement//21140169
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93488
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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spelling PONTIFICIA UNIVERSIDAD CATOLICA DE CHILEPardo Roa, Catalina2018https://hdl.handle.net/10533/232956http://purl.org/coar/access_right/c_abf2Otras Ciencias NaturalesCiencias Naturales“IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”Bueno, Susan MPONTIFICIA UNIVERSIDAD CATOLICA DE CHILEchilePardo Roa, Catalina2019-02-13T19:38:22Z2022-08-16T18:44:59Z2019-02-13T19:38:22Z2022-08-16T18:44:59Zinfo:eu-repo/date/embargoEnd/2020-01-012018Pathogenicity islands excision is a mechanism that occurs in several Enterobacteriaceae family members, including Salmonella enterica subspecie enterica serovars. ROD21 is an excisable pathogenicity island present in the chromosome of Salmonella enterica serovar Enteritidis and other serovars. It has been described that excision of ROD21 may play an important role in the ability of S. Enteritidis to cause systemic infection in the murine model, because strain unable to excise ROD21 showed a reduced ability to colonize the liver and the spleen. In this work, the kinetics of ROD21 excision in vivo across the stages of the infective cycle performed by S. Enteritidis in C57BL/6 mice was evaluated and the importance of ROD21 excision on Salmonella infection ability and gene expression was determined. After different times post infection, bacterial load and excision frequency was quantified in different portions of the digestive tract and internal organs. It was observed that the frequency of ROD21 excision increased significantly in the bacterial population able to colonize mesenteric lymph nodes at early stages, remaining very low in liver and spleen during these stages. However, a drastic increase in ROD21 excision was observed at late times post infection when intestinal re-infection and mortality start to happen. These observations suggest that the inability to excise ROD21 affects the translocation of S. Enteritidis from the intestine to the mesenteric lymph nodes due, possibly, to a defect in its capacity to invade host cells. On the other hand, the assays showed that impairment to excise ROD21 can affect the expression of genes located both within and outside the island. These results may be indicating that ROD21´s excision is a genetic mechanism employed by S. Enteritidis to regulate the expression of its virulence genes, to adapt to the conditions found in different tissues and to produce a successful systemic infection in the mouse.PFCHA-Becaslos datos de la tesis se encuentran en revisión para ser publicados en un journal.PFCHA-Becas21140169https://hdl.handle.net/10533/232956instname: Conicytreponame: Repositorio Digital RI2.0info:eu-repo/grantAgreement//21140169info:eu-repo/semantics/dataset/hdl.handle.net/10533/93488info:eu-repo/semantics/openAccessCiencias NaturalesOtras Ciencias Naturales“IN VIVO EVALUATION OF THE EXCISION OF PATHOGENICITY ISLAND ROD21 OF Salmonella enterica serovar Enteritidis AND ITS ROLE IN THE REGULATION OF GENES EXPRESSION DURING THE DIFFERENT STAGES OF THE INFECTION CYCLE”Tesis Magísterinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionTesisTesishttps://hdl.handle.net/10533/232956PFCHA-Becasba09c11f-6d35-40d9-93d9-1f8ccaa21d12virtual::54577-1ba09c11f-6d35-40d9-93d9-1f8ccaa21d12virtual::54577-1ORIGINAL23365804-9_Pardo_Catalina .pdfTesis Doctorado, Pardo_Roaapplication/pdf5885932https://repositorio.anid.cl/bitstreams/f638e4b0-5843-48b1-bb3f-e7b06f32ce73/download29791fab760d4a18309d9eaeab0c2d09MD51LICENSElicense.txttext/plain1779https://repositorio.anid.cl/bitstreams/313647c3-313b-4303-8d3d-03ebcfa4c752/download593a6e7305c66c56041a9f9e15a649c1MD52TEXT23365804-9_Pardo_Catalina .pdf.txtExtracted texttext/plain358293https://repositorio.anid.cl/bitstreams/5c8e6649-838f-4216-bdbd-df63e39c12ea/download2ddd41aa29f21a1d97cc9e4150983e9eMD53THUMBNAIL23365804-9_Pardo_Catalina .pdf.jpgIM Thumbnailimage/jpeg1590https://repositorio.anid.cl/bitstreams/614028ac-d1c0-4ec3-99d3-18b90f8c41fa/downloadbd01d010c0c303f0a046f4b50b9030f3MD5410533/232956oai:repositorio.anid.cl:10533/2329562023-07-24 05:44:04.129https://repositorio.anid.clRepositorio ANIDaletelier@anid.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