A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions

Piscirickettsia salmonis is the bacterium that causes Piscirickettsiosis, a systemic disease of salmonid fish responsible for significant economic losses within the aquaculture industry worldwide. The growth of the bacterium for vaccine formulation has been traditionally accomplished by infecting eu...

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Autores: Henriquez-Ulloa, Mirtha Andrea, Gonzalez-Romo, Ernesto Alejandro, Marshall-Gonzalez, Sergio Hernan, Henriquez-Quezada, VitaliaBbeatriz, Gomez-Carmona, Fernando Andres, Martinez-Basterrechea, Irene, Altamirano-Gomez, Claudia Victoria
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/197030
Acesso em linha:https://hdl.handle.net/10533/197030
Access Level:acceso abierto
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spelling Altamirano-Gomez, Claudia VictoriaMartinez-Basterrechea, IreneGomez-Carmona, Fernando AndresHenriquez-Quezada, VitaliaBbeatrizMarshall-Gonzalez, Sergio HernanGonzalez-Romo, Ernesto AlejandroHenriquez-Ulloa, Mirtha Andrea201310.1371/journal.pone.0071830https://hdl.handle.net/10533/197030http://purl.org/coar/access_right/c_abf2A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditionsUSAWASHINGTONHenriquez-Ulloa, Mirtha AndreaGonzalez-Romo, Ernesto AlejandroMarshall-Gonzalez, Sergio HernanHenriquez-Quezada, VitaliaBbeatrizGomez-Carmona, Fernando AndresMartinez-Basterrechea, IreneAltamirano-Gomez, Claudia Victoria2017-04-27T18:50:07Z2022-07-07T01:43:30Z2017-04-27T18:50:07Z2022-07-07T01:43:30Z2013Piscirickettsia salmonis is the bacterium that causes Piscirickettsiosis, a systemic disease of salmonid fish responsible for significant economic losses within the aquaculture industry worldwide. The growth of the bacterium for vaccine formulation has been traditionally accomplished by infecting eukaryotic cell lines, a process that involves high production costs and is time-consuming. Recent research has demonstrated that it is possible to culture pure P. salmonis in a blood containing (cell-free) medium. In the present work we demonstrate the growth of P. salmonis in a liquid medium free from blood and serum components, thus establishing a novel and simplified bacteriological medium. Additionally, the new media reported provides improved growth conditions for P. salmonis, where biomass concentrations of approximately 800 mg cell dry weight L-1 were obtained, about eight times higher than those reported for the blood containing medium. A 2-level full factorial design was employed to evaluate the significance of the main medium components on cell growth and an optimal temperature range of 23-27 degrees C was determined for the microorganism to grow in the novel liquid media. Therefore, these results represent a breakthrough regarding P. salmonis research in order to optimize pure P. salmonis growth in liquid blood and serum free medium.This work was supported by FONDEF Grant D10I1185 to CA, SM, IM, and VH; and by CONICYT Doctoral Scholarship to FG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.1FONDEFclaudia.altamirano@ucv.clFONDEF [D10I1185]; CONICYTFONDEF0D10I1185D10I1185virtual::44720-1WOS:000324481600013WOS:0003244816000132169-897Xhttps://hdl.handle.net/10533/197030AMER GEOPHYSICAL UNIONinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI2.010.1371/journal.pone.0071830info:eu-repo/grantAgreement/Fondef/D10I1185info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477https://doi.org/10.1371/journal.pone.0071830info:eu-repo/semantics/openAccessA novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditionsJOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERESJ GEOPHYS RES ATMOSArticuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionengArticulohttps://hdl.handle.net/10533/197030FONDEFhttp://purl.org/coar/resource_type/c_2df8fbb156570193-2f5e-4ecd-8380-394c906dab33virtual::44720-156570193-2f5e-4ecd-8380-394c906dab33virtual::44720-110533/197030oai:repositorio.anid.cl:10533/1970302023-07-24 18:00:48.822https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions
dc.title.journal.none.fl_str_mv JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES
dc.title.journalabbreviation.none.fl_str_mv J GEOPHYS RES ATMOS
title A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions
spellingShingle A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions
Henriquez-Ulloa, Mirtha Andrea
title_short A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions
title_full A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions
title_fullStr A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions
title_full_unstemmed A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions
title_sort A novel liquid medium for the efficient growth of the salmonid pathogen piscirickettsia salmonis and optimization of culture conditions
dc.creator.none.fl_str_mv Henriquez-Ulloa, Mirtha Andrea
Gonzalez-Romo, Ernesto Alejandro
Marshall-Gonzalez, Sergio Hernan
Henriquez-Quezada, VitaliaBbeatriz
Gomez-Carmona, Fernando Andres
Martinez-Basterrechea, Irene
Altamirano-Gomez, Claudia Victoria
author Henriquez-Ulloa, Mirtha Andrea
author_facet Henriquez-Ulloa, Mirtha Andrea
Gonzalez-Romo, Ernesto Alejandro
Marshall-Gonzalez, Sergio Hernan
Henriquez-Quezada, VitaliaBbeatriz
Gomez-Carmona, Fernando Andres
Martinez-Basterrechea, Irene
Altamirano-Gomez, Claudia Victoria
author_role author
author2 Gonzalez-Romo, Ernesto Alejandro
Marshall-Gonzalez, Sergio Hernan
Henriquez-Quezada, VitaliaBbeatriz
Gomez-Carmona, Fernando Andres
Martinez-Basterrechea, Irene
Altamirano-Gomez, Claudia Victoria
author2_role author
author
author
author
author
author
description Piscirickettsia salmonis is the bacterium that causes Piscirickettsiosis, a systemic disease of salmonid fish responsible for significant economic losses within the aquaculture industry worldwide. The growth of the bacterium for vaccine formulation has been traditionally accomplished by infecting eukaryotic cell lines, a process that involves high production costs and is time-consuming. Recent research has demonstrated that it is possible to culture pure P. salmonis in a blood containing (cell-free) medium. In the present work we demonstrate the growth of P. salmonis in a liquid medium free from blood and serum components, thus establishing a novel and simplified bacteriological medium. Additionally, the new media reported provides improved growth conditions for P. salmonis, where biomass concentrations of approximately 800 mg cell dry weight L-1 were obtained, about eight times higher than those reported for the blood containing medium. A 2-level full factorial design was employed to evaluate the significance of the main medium components on cell growth and an optimal temperature range of 23-27 degrees C was determined for the microorganism to grow in the novel liquid media. Therefore, these results represent a breakthrough regarding P. salmonis research in order to optimize pure P. salmonis growth in liquid blood and serum free medium.
publishDate 2013
dc.date.issued.none.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2017-04-27T18:50:07Z
2022-07-07T01:43:30Z
dc.date.available.none.fl_str_mv 2017-04-27T18:50:07Z
2022-07-07T01:43:30Z
dc.type.none.fl_str_mv Articulo
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.eissn.none.fl_str_mv 0
dc.identifier.folio.none.fl_str_mv D10I1185
D10I1185
dc.identifier.idwos.none.fl_str_mv WOS:000324481600013
dc.identifier.isi.none.fl_str_mv WOS:000324481600013
dc.identifier.issn.none.fl_str_mv 2169-897X
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/197030
identifier_str_mv 0
D10I1185
WOS:000324481600013
2169-897X
url https://hdl.handle.net/10533/197030
dc.language.*.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.1371/journal.pone.0071830
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondef/D10I1185
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relation.uri.none.fl_str_mv https://doi.org/10.1371/journal.pone.0071830
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.spatial.none.fl_str_mv WASHINGTON
dc.publisher.none.fl_str_mv AMER GEOPHYSICAL UNION
publisher.none.fl_str_mv AMER GEOPHYSICAL UNION
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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score 15.812429

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