Identification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.

Iron is an essential micronutrient for all living organisms. Embryo accumulates iron during seed maturation stages of embryogenesis. The role of iron in seed yields is an important agronomical trait because iron deficiency affects plant reproduction and limit crops yields. Using Arabidopsis thaliana...

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Detalles Bibliográficos
Autor: Ibeas Henriquez, Miguel Angel
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2020
País:Chile
OAI Identifier:oai:repositorio.anid.cl:10533/246399
Acceso en línea:https://hdl.handle.net/10533/246399
Access Level:acceso abierto
Palabra clave:Ciencias Naturales
Otras Ciencias Naturales
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dc.title.es_CL.fl_str_mv Identification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.
title Identification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.
spellingShingle Identification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.
Ibeas Henriquez, Miguel Angel
Ciencias Naturales
Otras Ciencias Naturales
title_short Identification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.
title_full Identification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.
title_fullStr Identification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.
title_full_unstemmed Identification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.
title_sort Identification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.
dc.creator.none.fl_str_mv Ibeas Henriquez, Miguel Angel
author Ibeas Henriquez, Miguel Angel
author_facet Ibeas Henriquez, Miguel Angel
author_role author
dc.contributor.advisor.none.fl_str_mv Roschzttardtz, Hannetz
dc.contributor.institution.es_CL.fl_str_mv PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE
dc.subject.oecd1n.es_CL.fl_str_mv Ciencias Naturales
topic Ciencias Naturales
Otras Ciencias Naturales
dc.subject.oecd2n.es_CL.fl_str_mv Otras Ciencias Naturales
description Iron is an essential micronutrient for all living organisms. Embryo accumulates iron during seed maturation stages of embryogenesis. The role of iron in seed yields is an important agronomical trait because iron deficiency affects plant reproduction and limit crops yields. Using Arabidopsis thaliana embryos, it has been described that iron accumulates in the vacuoles of the endodermis cell layer. Using Perls/DAB staining we were able to identify that this distribution pattern is conserved in different members from Brassicales. We extend this study to embryos belonging to species from different orders from Eudicotyledoneae. Our results suggest that iron pattern found in Arabidopsis is not extended to all Eudicotyledoneae. Noticeably, in Chenopodium quinoa embryos iron accumulates in several cell layers including cortex and endodermis cells. Interestingly, we detected high amount of phytoferritins in quinoa embryos. Chenopodium quinoa is a highly nutritious crop that is adapted to a wide range of ecosystems and it has reach international attention because of the nutritional value. We found that iron loading during Chenopodium quinoa seed development is different than what we observed before in embryos belonging to the Brassicaceae family. Ours results open new questions about the molecular mechanism controlling iron loading, distribution and accumulation in quinoa embryo seeds.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-10-28T18:00:14Z
2022-08-16T19:12:40Z
dc.date.available.none.fl_str_mv 2020-10-28T18:00:14Z
2022-08-16T19:12:40Z
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spelling PONTIFICIA UNIVERSIDAD CATOLICA DE CHILEIbeas Henriquez, Miguel Angel2020https://hdl.handle.net/10533/246399http://purl.org/coar/access_right/c_abf2Otras Ciencias NaturalesCiencias NaturalesIdentification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.Roschzttardtz, HannetzPONTIFICIA UNIVERSIDAD CATOLICA DE CHILEChileIbeas Henriquez, Miguel Angel2020-10-28T18:00:14Z2022-08-16T19:12:40Z2020-10-28T18:00:14Z2022-08-16T19:12:40Z2020Iron is an essential micronutrient for all living organisms. Embryo accumulates iron during seed maturation stages of embryogenesis. The role of iron in seed yields is an important agronomical trait because iron deficiency affects plant reproduction and limit crops yields. Using Arabidopsis thaliana embryos, it has been described that iron accumulates in the vacuoles of the endodermis cell layer. Using Perls/DAB staining we were able to identify that this distribution pattern is conserved in different members from Brassicales. We extend this study to embryos belonging to species from different orders from Eudicotyledoneae. Our results suggest that iron pattern found in Arabidopsis is not extended to all Eudicotyledoneae. Noticeably, in Chenopodium quinoa embryos iron accumulates in several cell layers including cortex and endodermis cells. Interestingly, we detected high amount of phytoferritins in quinoa embryos. Chenopodium quinoa is a highly nutritious crop that is adapted to a wide range of ecosystems and it has reach international attention because of the nutritional value. We found that iron loading during Chenopodium quinoa seed development is different than what we observed before in embryos belonging to the Brassicaceae family. Ours results open new questions about the molecular mechanism controlling iron loading, distribution and accumulation in quinoa embryo seeds.El hierro es un micronutriente esencial para casi todos los organismos vivos. Los embriones acumulan hierro durante la etapa de maduración de la semilla de la embriogénesis. El déficit de hierro es un gran problema agronómico ya que afecta la rdesarrollo de las plantas y limita de fertilidad y rendimiento de los cultivos. Utilizando embriones de Arabidopsis thaliana ha sido posible determinar que el hierro se acumula específicamente en las vacuolas de las células de la endodermis. Utilizando la tinción Perls/DAB, fuimos capaces de identificar que este patrón de distribución es diferente en otros miembros de las Brassicales. Nosotros extendimos este estudio a embriones de especies pertenecientes a distintos órdenes de las Eudicotyledoneae. Nuestros resultados sugieren que el patrón de distribución de hierro observado en embriones de Arabidopsis no es conservado en otras Eudicotyledoneae. Notablemente, en embriones de Chenopodium quinoa el hierro se acumula en distintas capas celulares incluyendo las células del cortex y la endodermis. Interesantemente, identificados una gran cantidad de fitoferritinas en embriones de quinoa. Chenopodium quinoa es un pseudo-cereal de origen andino muy nutritivo que se caracteriza por crecer en diversos ecosistemas y ha alcanzado un alto nivel de atención debido a lo nutritivas de sus semillas. Por otro lado, nosotros observamos que durante el desarrollo de la semilla de Chenopodium quinoa el hierro se carga de una manera diferente a lo que observamos en el desarrollo de semillas de Brassica napus. Nuestros resultados abren nuevas preguntas respecto a cuáles son los mecanismos que controlan la carga, distribución y acumulación de hierro en semillas.21160350https://hdl.handle.net/10533/246399instname: Conicytreponame: Repositorio Digital RI2.0info:eu-repo/grantAgreement//21160350info:eu-repo/semantics/dataset/hdl.handle.net/10533/93488https://repositorio.uc.cl/handle/11534/45380info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivs 3.0 Chilehttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/Ciencias NaturalesOtras Ciencias NaturalesIdentification and characterization of molecular mechanisms controlling iron distribution and accumulation during seed development in Chenopodium quinoa.info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionTesisTesishttps://hdl.handle.net/10533/2463990c696a2b-8056-406c-973f-5cac88d9244bvirtual::57938-10c696a2b-8056-406c-973f-5cac88d9244bvirtual::57938-1LICENSElicense.txttext/plain1779https://repositorio.anid.cl/bitstreams/44e54451-08cf-4b4a-a14c-9fa37bc48978/download593a6e7305c66c56041a9f9e15a649c1MD51CC-LICENSElicense_rdfapplication/octet-stream1232https://repositorio.anid.cl/bitstreams/ffe78fd0-15b7-448b-b69f-e6b1f6f564f3/downloadf97bcfdf58f3e17b5cec231112dab5b1MD52ORIGINALTesis_Miguel_Ibeas_ANID.pdfTesis Doctoralapplication/pdf17655895https://repositorio.anid.cl/bitstreams/3b04208f-0817-45c3-be6a-460eeaad1715/downloaddd119e9969d22702bec2fa71625027b0MD53TEXTTesis_Miguel_Ibeas_ANID.pdf.txtExtracted texttext/plain112608https://repositorio.anid.cl/bitstreams/2b98faed-628e-4e54-b37f-3ca47556129a/download5b8d49c0fe164905fb5f7d003d0ad3dbMD54THUMBNAILTesis_Miguel_Ibeas_ANID.pdf.jpgIM Thumbnailimage/jpeg4592https://repositorio.anid.cl/bitstreams/af5bd36b-5cb8-4e52-ae4d-4d145a21b6bb/downloadd1229625823afa6d03478036d80c8c36MD5510533/246399oai:repositorio.anid.cl:10533/2463992023-07-24 09:16:59.512http://creativecommons.org/licenses/by-nc-nd/3.0/cl/info:eu-repo/semantics/openAccesshttps://repositorio.anid.clRepositorio ANIDaletelier@anid.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