Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques

The adhesion of microorganisms to minerals is one of the least understood aspects of bioleaching processes. Mineral and microorganism type are determinant factors affecting the capacity of a microbial community to form biofilms in these processes. In this paper the adhesion capacity of different mic...

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Detalles Bibliográficos
Autores: Demergasso-Semenzato, Cecilia, Echeverria-Rivas, Araceli
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/198032
Acceso en línea:https://hdl.handle.net/10533/198032
Access Level:acceso abierto
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spelling Echeverria-Rivas, AraceliDemergasso-Semenzato, Cecilia20090https://hdl.handle.net/10533/198032http://purl.org/coar/access_right/c_abf2Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques8683CHESTAFA-ZURICHDemergasso-Semenzato, CeciliaEcheverria-Rivas, Araceli2017-04-27T18:54:02Z2022-07-07T02:28:13Z2017-04-27T18:54:02Z2022-07-07T02:28:13Z2009The adhesion of microorganisms to minerals is one of the least understood aspects of bioleaching processes. Mineral and microorganism type are determinant factors affecting the capacity of a microbial community to form biofilms in these processes. In this paper the adhesion capacity of different microorganisms to different copper sulfide minerals generally present in bioleaching processes was studied. The minerals, chalcosite, chalcopyrite and pyrite were used and the composition of the microbial Population which adhered to the mineral surfaces was determined using the Catalyzed Reporter Deposition Fluorescent In Situ Hybridization (CARD-FISH) technique. This technique involves the fluorescent marking of the cells using specific DNA probes and their observation with a confocal microscope. Three probes were used against the microorganisms: Acidithiobacillus ferooxidans, Acidithiobacillus thiooxidans and Leptospirillum. Polished fragments of the three minerals were placed in a tube with a culture medium which was inoculated with cells from a mixed culture capable of growing at 25 degrees C. The adhered microorganisms were counted with CARD-FISH and compared with the total count which was carried out with DAPI staining. The results show that microorganisms adhered indistinctly to pyrite and chalcopyrite but not chalcosite. It was also observed that in pyrite 60% of the adhered microorganisms were Acidithiobacillus thiooxidans, 35% Acidithiobacillus ferrooxidans, and 1% Leptospirillum. The remaining 4% were unidentified microorganisms.05FONDEFaecheverri@ucn.cl; cdemerga@ucn.cl0FONDEF730D04I1169D04I1169virtual::36311-1WOS:000273541600016WOS:0002735416000161022-6680https://hdl.handle.net/10533/198032TRANS TECH PUBLICATIONS LTDinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI2.0010.4028/www.scientific.net/AMR.71-73.83info:eu-repo/grantAgreement/Fondef/D04I1169info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477https://doi.org/10.4028/www.scientific.net/AMR.71-73.83info:eu-repo/semantics/openAccessAssessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniquesADVANCED MATERIALS RESEARCHADV MAT RES Articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionengArticulohttps://hdl.handle.net/10533/198032FONDEFhttp://purl.org/coar/resource_type/c_2df8fbb117f6ef8c-0a2d-4057-98aa-80ef4e80efd3virtual::36311-117f6ef8c-0a2d-4057-98aa-80ef4e80efd3virtual::36311-110533/198032oai:repositorio.anid.cl:10533/1980322023-07-24 16:05:12.539https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques
dc.title.journal.none.fl_str_mv ADVANCED MATERIALS RESEARCH
dc.title.journalabbreviation.none.fl_str_mv ADV MAT RES 
title Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques
spellingShingle Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques
Demergasso-Semenzato, Cecilia
title_short Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques
title_full Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques
title_fullStr Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques
title_full_unstemmed Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques
title_sort Assessment of microbial adhesion in mixed cultures to sulfide minerals using card-fish techniques
dc.creator.none.fl_str_mv Demergasso-Semenzato, Cecilia
Echeverria-Rivas, Araceli
author Demergasso-Semenzato, Cecilia
author_facet Demergasso-Semenzato, Cecilia
Echeverria-Rivas, Araceli
author_role author
author2 Echeverria-Rivas, Araceli
author2_role author
description The adhesion of microorganisms to minerals is one of the least understood aspects of bioleaching processes. Mineral and microorganism type are determinant factors affecting the capacity of a microbial community to form biofilms in these processes. In this paper the adhesion capacity of different microorganisms to different copper sulfide minerals generally present in bioleaching processes was studied. The minerals, chalcosite, chalcopyrite and pyrite were used and the composition of the microbial Population which adhered to the mineral surfaces was determined using the Catalyzed Reporter Deposition Fluorescent In Situ Hybridization (CARD-FISH) technique. This technique involves the fluorescent marking of the cells using specific DNA probes and their observation with a confocal microscope. Three probes were used against the microorganisms: Acidithiobacillus ferooxidans, Acidithiobacillus thiooxidans and Leptospirillum. Polished fragments of the three minerals were placed in a tube with a culture medium which was inoculated with cells from a mixed culture capable of growing at 25 degrees C. The adhered microorganisms were counted with CARD-FISH and compared with the total count which was carried out with DAPI staining. The results show that microorganisms adhered indistinctly to pyrite and chalcopyrite but not chalcosite. It was also observed that in pyrite 60% of the adhered microorganisms were Acidithiobacillus thiooxidans, 35% Acidithiobacillus ferrooxidans, and 1% Leptospirillum. The remaining 4% were unidentified microorganisms.
publishDate 2009
dc.date.issued.none.fl_str_mv 2009
dc.date.accessioned.none.fl_str_mv 2017-04-27T18:54:02Z
2022-07-07T02:28:13Z
dc.date.available.none.fl_str_mv 2017-04-27T18:54:02Z
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dc.coverage.spatial.none.fl_str_mv STAFA-ZURICH
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