Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray

The bioleaching of metal sulfide has developed into a very important industrial process and understanding the microbial dynamic is key to advancing commercial bioleaching operations. Here we report the first quantitative description of the dynamic of active communities in an industrial bioleaching h...

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Autores: Demergasso-Semenzato, Cecilia, Acosta-Grinok, Mauricio, Galleguillos-Guajardo, Felipe, Moreno-Paz, M.,, Parro, Víctor, Remonsellez-Fuentes, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Chile
Institución:Agencia Nacional de Investigación y Desarrollo
Repositorio:Articulos_ANID
Idioma:ENG
OAI Identifier:oai:repositorio.anid.cl:10533/198036
Acceso en línea:https://hdl.handle.net/10533/198036
Access Level:acceso abierto
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spelling Remonsellez-Fuentes, FranciscoParro, VíctorMoreno-Paz, M.,Galleguillos-Guajardo, FelipeAcosta-Grinok, MauricioDemergasso-Semenzato, Cecilia200910.1111/j.1751-7915.2009.00112.xhttps://hdl.handle.net/10533/198036http://purl.org/coar/access_right/c_abf2Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray624613Demergasso-Semenzato, CeciliaAcosta-Grinok, MauricioGalleguillos-Guajardo, FelipeMoreno-Paz, M.,Parro, VíctorRemonsellez-Fuentes, Francisco2017-04-27T18:54:03Z2022-07-07T02:28:16Z2017-04-27T18:54:03Z2022-07-07T02:28:16Z2009The bioleaching of metal sulfide has developed into a very important industrial process and understanding the microbial dynamic is key to advancing commercial bioleaching operations. Here we report the first quantitative description of the dynamic of active communities in an industrial bioleaching heap. Acidithiobacillus ferrooxidans was the most abundant during the first part of the leaching cycle, while the abundance of Leptospirillum ferriphilum and Ferroplasma acidiphilum increased with age of the heap. Acidithiobacillus thiooxidans kept constant throughout the leaching cycle, and Firmicutes group showed a low and a patchy distribution in the heap. The Acidiphilium-like bacteria reached their highest abundance corresponding to the amount of autotrophs. The active microorganisms in the leaching system were determined using two RNA-based sensitive techniques. In most cases, the 16S rRNA copy numbers of At. ferrooxidans, L. ferriphilum, At. thiooxidans and F. acidiphilum, was concomitant with the DNA copy numbers, whereas Acidiphilium-like bacteria and some Firmicutes members did not show a clear correlation between 16S rRNA accumulation and DNA copy numbers. However, the prokaryotic acidophile microarray (PAM) analysis showed active members of Alphaproteobacteria in all samples and of Sulfobacillus genus in older ones. Also, new active groups such as Actinobacteria and Acidobacterium genus were detected by PAM. The results suggest that changes during the leaching cycle in chemical and physical conditions, such as pH and Fe3+/Fe2+ ion rate, are primary factors shaping the microbial dynamic in the heap.This work was supported in part by FONDEF Proyect D04I1169 from CONICYT and a technological stay in the Centro de Astrobiologia (CSIC-INTA) from CORFO. F. Remonsellez is supported in part by the Technology and Science Bicentennial Program from CONICYT. V. Parro had a 'Ramon y Cajal' contract from the Spanish Ministerio de Ciencia e Innovacion. We thank Alex Echeverria for his help in the phylogenetic tree analysis.25FONDEFcdemerga@ucn.clCONICYT [D04I1169]; CORFO; Spanish Ministerio de Ciencia e Innovacion6FONDEF20D04I1169D04I1169virtual::19131-1WOS:000207904200005WOS:0002079042000051751-7915https://hdl.handle.net/10533/198036ENGinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI2.010.1111/j.1751-7915.2009.00112.xinfo:eu-repo/grantAgreement/Fondef/D04I1169info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477https://doi.org/10.1111/j.1751-7915.2009.00112.xinfo:eu-repo/semantics/openAccessDynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarrayMICROBIAL BIOTECHNOLOGY (ONLINE)MICROB BIOTECHNOLArticuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:Articulos_ANIDinstname:Agencia Nacional de Investigación y Desarrolloinstacron:ANIDArticulohttps://hdl.handle.net/10533/198036FONDEFhttp://purl.org/coar/resource_type/c_2df8fbb117f6ef8c-0a2d-4057-98aa-80ef4e80efd3virtual::19131-117f6ef8c-0a2d-4057-98aa-80ef4e80efd3virtual::19131-110533/1980362023-07-24 19:11:12.699oai:repositorio.anid.cl:10533/198036https://repositorio.anid.clopendoar:2023-07-24T19:11:12Articulos_ANID - Agencia Nacional de Investigación y Desarrollofalse
dc.title.none.fl_str_mv Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray
dc.title.journal.none.fl_str_mv MICROBIAL BIOTECHNOLOGY (ONLINE)
dc.title.journalabbreviation.none.fl_str_mv MICROB BIOTECHNOL
title Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray
spellingShingle Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray
Demergasso-Semenzato, Cecilia
title_short Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray
title_full Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray
title_fullStr Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray
title_full_unstemmed Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray
title_sort Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray
dc.creator.none.fl_str_mv Demergasso-Semenzato, Cecilia
Acosta-Grinok, Mauricio
Galleguillos-Guajardo, Felipe
Moreno-Paz, M.,
Parro, Víctor
Remonsellez-Fuentes, Francisco
author Demergasso-Semenzato, Cecilia
author_facet Demergasso-Semenzato, Cecilia
Acosta-Grinok, Mauricio
Galleguillos-Guajardo, Felipe
Moreno-Paz, M.,
Parro, Víctor
Remonsellez-Fuentes, Francisco
author_role author
author2 Acosta-Grinok, Mauricio
Galleguillos-Guajardo, Felipe
Moreno-Paz, M.,
Parro, Víctor
Remonsellez-Fuentes, Francisco
author2_role author
author
author
author
author
description The bioleaching of metal sulfide has developed into a very important industrial process and understanding the microbial dynamic is key to advancing commercial bioleaching operations. Here we report the first quantitative description of the dynamic of active communities in an industrial bioleaching heap. Acidithiobacillus ferrooxidans was the most abundant during the first part of the leaching cycle, while the abundance of Leptospirillum ferriphilum and Ferroplasma acidiphilum increased with age of the heap. Acidithiobacillus thiooxidans kept constant throughout the leaching cycle, and Firmicutes group showed a low and a patchy distribution in the heap. The Acidiphilium-like bacteria reached their highest abundance corresponding to the amount of autotrophs. The active microorganisms in the leaching system were determined using two RNA-based sensitive techniques. In most cases, the 16S rRNA copy numbers of At. ferrooxidans, L. ferriphilum, At. thiooxidans and F. acidiphilum, was concomitant with the DNA copy numbers, whereas Acidiphilium-like bacteria and some Firmicutes members did not show a clear correlation between 16S rRNA accumulation and DNA copy numbers. However, the prokaryotic acidophile microarray (PAM) analysis showed active members of Alphaproteobacteria in all samples and of Sulfobacillus genus in older ones. Also, new active groups such as Actinobacteria and Acidobacterium genus were detected by PAM. The results suggest that changes during the leaching cycle in chemical and physical conditions, such as pH and Fe3+/Fe2+ ion rate, are primary factors shaping the microbial dynamic in the heap.
publishDate 2009
dc.date.issued.none.fl_str_mv 2009
dc.date.accessioned.none.fl_str_mv 2017-04-27T18:54:03Z
2022-07-07T02:28:16Z
dc.date.available.none.fl_str_mv 2017-04-27T18:54:03Z
2022-07-07T02:28:16Z
dc.type.none.fl_str_mv Articulo
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dc.identifier.folio.none.fl_str_mv D04I1169
D04I1169
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dc.identifier.issn.none.fl_str_mv 1751-7915
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reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.1111/j.1751-7915.2009.00112.x
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondef/D04I1169
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relation.uri.none.fl_str_mv https://doi.org/10.1111/j.1751-7915.2009.00112.x
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