Bacterial diversity in the oxygen minimum zone of the eastern tropical South Pacific

The structure and diversity of bacterial communities associated with the oxygen minimum zone (OMZ) of the eastern tropical South Pacific was studied through phylogenetic analysis. Clone libraries of 16S rRNA gene fragments were constructed using environmental DNA collected from the OMZ (60 m and 200...

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Detalles Bibliográficos
Autores: Stevens, H., Ulloa, O.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/237529
Acceso en línea:https://hdl.handle.net/10533/237529
Access Level:acceso abierto
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spelling Ulloa, O.Stevens, H.200810.1111/j.1462-2920.2007.01539.xhttps://hdl.handle.net/10533/237529http://purl.org/coar/access_right/c_abf2Bacterial diversity in the oxygen minimum zone of the eastern tropical South PacificStevens, H.Ulloa, O.2019-12-18T18:15:44Z2022-07-07T23:41:13Z2019-12-18T18:15:44Z2022-07-07T23:41:13Z2008The structure and diversity of bacterial communities associated with the oxygen minimum zone (OMZ) of the eastern tropical South Pacific was studied through phylogenetic analysis. Clone libraries of 16S rRNA gene fragments were constructed using environmental DNA collected from the OMZ (60 m and 200 m), the sea surface (10 m), and the deep oxycline (450 m). At the class level, the majority of sequences affiliated to the gamma- (53.7%) and alpha-Proteobacteria (19.7%), and to the Bacteroidetes (11.2%). A vertical partitioning of the bacterial communities was observed, with main differences between the suboxic OMZ and the more oxygenated surface and deep oxycline waters. At the surface, the microbial community was predominantly characterized by SAR86, Loktanella and unclassified Flavobacteriaceae, whereas the deeper layer was dominated by Sulfitobacter and unclassified Alteromonadaceae. In the OMZ, major constituents affiliated to the marine SAR11 clade and to thiotrophic gamma-symbionts (25% of all sequences), a group not commonly found in pelagic waters. Sequences affiliating to the phylum Chloroflexi, to the AGG47 and SAR202 clades, to the delta-Proteobacteria, to the Acidobacteria, and to the 'anammox group' of the Planctomycetes were found exclusively in the OMZ. The bacterial richness in the OMZ was higher than in the oxic surface and deeper oxycline, as revealed by rarefaction analysis and the Chao1 richness estimator (surface: 45 +/- 8, deeper oxycline: 76 +/- 26; OMZ (60 m): 97 +/- 33, OMZ (200 m): 109 +/- 31). OMZ bacterial diversity indices (Fisher's: approximately 30 +/- 5, Shannon's: approximately 3.31, inverse Simpson's: approximately 20) were similar to those found in other pelagic marine environments. Thus, our results indicate a distinct and diverse bacterial community within the OMZ, with presumably novel and yet uncultivated bacterial lineages.FONDAPFONDAP1501000715010007virtual::18246-1WOS:000254666700013https://hdl.handle.net/10533/237529enginstname: Conicytreponame: Repositorio Digital RI2.010.1111/j.1462-2920.2007.01539.xinfo:eu-repo/grantAgreement/Fondap/15010007https://www.ncbi.nlm.nih.gov/pubmed/18294206Atribución-NoComercial-SinDerivadas 3.0 Chilehttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/info:eu-repo/semantics/openAccessBacterial diversity in the oxygen minimum zone of the eastern tropical South PacificEnvironmental MicrobiologyArticuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttps://hdl.handle.net/10533/237529http://purl.org/coar/resource_type/c_2df8fbb1dae0fa47-4504-443f-9116-d46805ce536evirtual::18246-1dae0fa47-4504-443f-9116-d46805ce536evirtual::18246-110533/237529oai:repositorio.anid.cl:10533/2375292023-07-24 20:02:19.757https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv Bacterial diversity in the oxygen minimum zone of the eastern tropical South Pacific
dc.title.journal.none.fl_str_mv Environmental Microbiology
title Bacterial diversity in the oxygen minimum zone of the eastern tropical South Pacific
spellingShingle Bacterial diversity in the oxygen minimum zone of the eastern tropical South Pacific
Stevens, H.
title_short Bacterial diversity in the oxygen minimum zone of the eastern tropical South Pacific
title_full Bacterial diversity in the oxygen minimum zone of the eastern tropical South Pacific
title_fullStr Bacterial diversity in the oxygen minimum zone of the eastern tropical South Pacific
title_full_unstemmed Bacterial diversity in the oxygen minimum zone of the eastern tropical South Pacific
title_sort Bacterial diversity in the oxygen minimum zone of the eastern tropical South Pacific
dc.creator.none.fl_str_mv Stevens, H.
Ulloa, O.
author Stevens, H.
author_facet Stevens, H.
Ulloa, O.
author_role author
author2 Ulloa, O.
author2_role author
description The structure and diversity of bacterial communities associated with the oxygen minimum zone (OMZ) of the eastern tropical South Pacific was studied through phylogenetic analysis. Clone libraries of 16S rRNA gene fragments were constructed using environmental DNA collected from the OMZ (60 m and 200 m), the sea surface (10 m), and the deep oxycline (450 m). At the class level, the majority of sequences affiliated to the gamma- (53.7%) and alpha-Proteobacteria (19.7%), and to the Bacteroidetes (11.2%). A vertical partitioning of the bacterial communities was observed, with main differences between the suboxic OMZ and the more oxygenated surface and deep oxycline waters. At the surface, the microbial community was predominantly characterized by SAR86, Loktanella and unclassified Flavobacteriaceae, whereas the deeper layer was dominated by Sulfitobacter and unclassified Alteromonadaceae. In the OMZ, major constituents affiliated to the marine SAR11 clade and to thiotrophic gamma-symbionts (25% of all sequences), a group not commonly found in pelagic waters. Sequences affiliating to the phylum Chloroflexi, to the AGG47 and SAR202 clades, to the delta-Proteobacteria, to the Acidobacteria, and to the 'anammox group' of the Planctomycetes were found exclusively in the OMZ. The bacterial richness in the OMZ was higher than in the oxic surface and deeper oxycline, as revealed by rarefaction analysis and the Chao1 richness estimator (surface: 45 +/- 8, deeper oxycline: 76 +/- 26; OMZ (60 m): 97 +/- 33, OMZ (200 m): 109 +/- 31). OMZ bacterial diversity indices (Fisher's: approximately 30 +/- 5, Shannon's: approximately 3.31, inverse Simpson's: approximately 20) were similar to those found in other pelagic marine environments. Thus, our results indicate a distinct and diverse bacterial community within the OMZ, with presumably novel and yet uncultivated bacterial lineages.
publishDate 2008
dc.date.issued.none.fl_str_mv 2008
dc.date.accessioned.none.fl_str_mv 2019-12-18T18:15:44Z
2022-07-07T23:41:13Z
dc.date.available.none.fl_str_mv 2019-12-18T18:15:44Z
2022-07-07T23:41:13Z
dc.type.none.fl_str_mv Articulo
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.folio.none.fl_str_mv 15010007
15010007
dc.identifier.idwos.none.fl_str_mv WOS:000254666700013
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/237529
identifier_str_mv 15010007
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url https://hdl.handle.net/10533/237529
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.1111/j.1462-2920.2007.01539.x
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondap/15010007
dc.relation.uri.none.fl_str_mv https://www.ncbi.nlm.nih.gov/pubmed/18294206
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 3.0 Chile
http://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 3.0 Chile
http://creativecommons.org/licenses/by-nc-nd/3.0/cl/
eu_rights_str_mv openAccess
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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