Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella

Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other ge...

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Autores: Valenzuela-Valdes, Pablo, Uribe-Campos, Paulina, Fuentes-Contreras, Daniela, Holmes, David, Shmaryahu, Amir, Valdes-Anabalon, Jorge, Zúñiga, Alicia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/197791
Acceso en línea:https://hdl.handle.net/10533/197791
Access Level:acceso abierto
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spelling Zúñiga, AliciaValdes-Anabalon, JorgeShmaryahu, AmirHolmes, DavidFuentes-Contreras, DanielaUribe-Campos, PaulinaValenzuela-Valdes, Pablo200810.1007/s10126-008-9107-8https://hdl.handle.net/10533/197791http://purl.org/coar/access_right/c_abf2Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella700692USANEW YORKValenzuela-Valdes, PabloUribe-Campos, PaulinaFuentes-Contreras, DanielaHolmes, DavidShmaryahu, AmirValdes-Anabalon, JorgeZúñiga, Alicia2017-04-27T18:52:57Z2022-07-07T02:25:14Z2017-04-27T18:52:57Z2022-07-07T02:25:14Z2008Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6% of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357-464203).This research has been partially funded by CONICYT-FONDEF Project MR02I1003 and by a Microsoft Research Joint R&D Program.23FONDEFpvalenzu@bionova.clCONICYT-FONDEF [MR02I1003]; Microsoft Research Joint RD Program6FONDEF100MR02I1003MR02I1003virtual::34431-1WOS:000260838600007WOS:0002608386000071436-2228https://hdl.handle.net/10533/197791SPRINGERinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI2.010.1007/s10126-008-9107-8info:eu-repo/grantAgreement/Fondef/MR02I1003info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477https://doi.org/10.1007/s10126-008-9107-8info:eu-repo/semantics/openAccessPreparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenellaMARINE BIOTECHNOLOGY (PRINT)Mar. Biotechnol.Articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionengArticulohttps://hdl.handle.net/10533/197791FONDEFhttp://purl.org/coar/resource_type/c_2df8fbb1e0af2358-5236-4345-b7d5-c177462cb9e9virtual::34431-1e0af2358-5236-4345-b7d5-c177462cb9e9virtual::34431-110533/197791oai:repositorio.anid.cl:10533/1977912023-07-24 17:23:17.252https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella
dc.title.journal.none.fl_str_mv MARINE BIOTECHNOLOGY (PRINT)
dc.title.journalabbreviation.none.fl_str_mv Mar. Biotechnol.
title Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella
spellingShingle Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella
Valenzuela-Valdes, Pablo
title_short Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella
title_full Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella
title_fullStr Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella
title_full_unstemmed Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella
title_sort Preparation and analysis of an expressed sequence tag library from the toxic dinoflagellate alexandrium catenella
dc.creator.none.fl_str_mv Valenzuela-Valdes, Pablo
Uribe-Campos, Paulina
Fuentes-Contreras, Daniela
Holmes, David
Shmaryahu, Amir
Valdes-Anabalon, Jorge
Zúñiga, Alicia
author Valenzuela-Valdes, Pablo
author_facet Valenzuela-Valdes, Pablo
Uribe-Campos, Paulina
Fuentes-Contreras, Daniela
Holmes, David
Shmaryahu, Amir
Valdes-Anabalon, Jorge
Zúñiga, Alicia
author_role author
author2 Uribe-Campos, Paulina
Fuentes-Contreras, Daniela
Holmes, David
Shmaryahu, Amir
Valdes-Anabalon, Jorge
Zúñiga, Alicia
author2_role author
author
author
author
author
author
description Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6% of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357-464203).
publishDate 2008
dc.date.issued.none.fl_str_mv 2008
dc.date.accessioned.none.fl_str_mv 2017-04-27T18:52:57Z
2022-07-07T02:25:14Z
dc.date.available.none.fl_str_mv 2017-04-27T18:52:57Z
2022-07-07T02:25:14Z
dc.type.none.fl_str_mv Articulo
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.eissn.none.fl_str_mv 0
dc.identifier.folio.none.fl_str_mv MR02I1003
MR02I1003
dc.identifier.idwos.none.fl_str_mv WOS:000260838600007
dc.identifier.isi.none.fl_str_mv WOS:000260838600007
dc.identifier.issn.none.fl_str_mv 1436-2228
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/197791
identifier_str_mv 0
MR02I1003
WOS:000260838600007
1436-2228
url https://hdl.handle.net/10533/197791
dc.language.*.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.1007/s10126-008-9107-8
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondef/MR02I1003
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relation.uri.none.fl_str_mv https://doi.org/10.1007/s10126-008-9107-8
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.spatial.none.fl_str_mv NEW YORK
dc.publisher.none.fl_str_mv SPRINGER
publisher.none.fl_str_mv SPRINGER
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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