Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation

Fibrotic disorders are typified by excessive connective tissue and extracellular matrix (ECM) deposition that precludes normal healing processes of different tissues. Connective tissue growth factor (CTGF) seems to be involved in the fibrotic response. Several muscular dystrophies are characterized...

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Autores: Vial. C, Zúñiga, L.M., Cabello-Verrugio, C., Cañón, P., Fadic, R., Brandan, E.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/236904
Acceso en línea:https://hdl.handle.net/10533/236904
Access Level:acceso abierto
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spelling Brandan, E.Fadic, R.Cañón, P.Cabello-Verrugio, C.Zúñiga, L.M.Vial. C200810.1002/jcp.21324https://hdl.handle.net/10533/236904http://purl.org/coar/access_right/c_abf2Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiationVial. CZúñiga, L.M.Cabello-Verrugio, C.Cañón, P.Fadic, R.Brandan, E.2019-12-18T18:14:17Z2022-07-07T21:57:40Z2019-12-18T18:14:17Z2022-07-07T21:57:40Z2008Fibrotic disorders are typified by excessive connective tissue and extracellular matrix (ECM) deposition that precludes normal healing processes of different tissues. Connective tissue growth factor (CTGF) seems to be involved in the fibrotic response. Several muscular dystrophies are characterized by a progressive weakness and wasting of the musculature, and by extensive fibrosis. However, the exact role of CTGF in skeletal muscle is unknown. Here we show that myoblasts and myotubes are able to synthesize CTGF in response to transforming growth factor type-beta (TGF-beta) and lysophosphatidic acid (LPA). CTGF induced several ECM constituents such as fibronectin, collagen type I and alpha4, 5, 6, and beta1 integrin subunits in myoblasts and myotubes. CTGF had an important inhibitory effect on muscle differentiation evaluated by the decrease in the nuclear translocation of the early muscle regulatory factor myogenin and myosin. Remarkable, CTGF treatment of myoblasts induced their dedifferentiation, characterized by down regulating MyoD and desmin, two markers of committed myoblasts, together with a strong reorganization of cytoskeletal filaments. These results provide novel evidence for the underlying mechanisms and participation of skeletal muscle cells in the synthesis and role of CTGF inducing fibrosis, inhibiting myogenesis and dedifferentiating myoblasts.FONDAPFONDAP1398000113980001virtual::23740-1WOS:000254887100014https://hdl.handle.net/10533/236904enginstname: Conicytreponame: Repositorio Digital RI2.010.1002/jcp.21324info:eu-repo/grantAgreement/Fondap/13980001https://www.ncbi.nlm.nih.gov/pubmed/18064627Atribución-NoComercial-SinDerivadas 3.0 Chilehttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/info:eu-repo/semantics/openAccessSkeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiationJ Cell PhysiolArticuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttps://hdl.handle.net/10533/236904http://purl.org/coar/resource_type/c_2df8fbb14c0c28ba-308a-47c1-afb7-2607f38e4c08virtual::23740-14c0c28ba-308a-47c1-afb7-2607f38e4c08virtual::23740-110533/236904oai:repositorio.anid.cl:10533/2369042023-07-24 18:45:46.499https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation
dc.title.journal.none.fl_str_mv J Cell Physiol
title Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation
spellingShingle Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation
Vial. C
title_short Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation
title_full Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation
title_fullStr Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation
title_full_unstemmed Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation
title_sort Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation
dc.creator.none.fl_str_mv Vial. C
Zúñiga, L.M.
Cabello-Verrugio, C.
Cañón, P.
Fadic, R.
Brandan, E.
author Vial. C
author_facet Vial. C
Zúñiga, L.M.
Cabello-Verrugio, C.
Cañón, P.
Fadic, R.
Brandan, E.
author_role author
author2 Zúñiga, L.M.
Cabello-Verrugio, C.
Cañón, P.
Fadic, R.
Brandan, E.
author2_role author
author
author
author
author
description Fibrotic disorders are typified by excessive connective tissue and extracellular matrix (ECM) deposition that precludes normal healing processes of different tissues. Connective tissue growth factor (CTGF) seems to be involved in the fibrotic response. Several muscular dystrophies are characterized by a progressive weakness and wasting of the musculature, and by extensive fibrosis. However, the exact role of CTGF in skeletal muscle is unknown. Here we show that myoblasts and myotubes are able to synthesize CTGF in response to transforming growth factor type-beta (TGF-beta) and lysophosphatidic acid (LPA). CTGF induced several ECM constituents such as fibronectin, collagen type I and alpha4, 5, 6, and beta1 integrin subunits in myoblasts and myotubes. CTGF had an important inhibitory effect on muscle differentiation evaluated by the decrease in the nuclear translocation of the early muscle regulatory factor myogenin and myosin. Remarkable, CTGF treatment of myoblasts induced their dedifferentiation, characterized by down regulating MyoD and desmin, two markers of committed myoblasts, together with a strong reorganization of cytoskeletal filaments. These results provide novel evidence for the underlying mechanisms and participation of skeletal muscle cells in the synthesis and role of CTGF inducing fibrosis, inhibiting myogenesis and dedifferentiating myoblasts.
publishDate 2008
dc.date.issued.none.fl_str_mv 2008
dc.date.accessioned.none.fl_str_mv 2019-12-18T18:14:17Z
2022-07-07T21:57:40Z
dc.date.available.none.fl_str_mv 2019-12-18T18:14:17Z
2022-07-07T21:57:40Z
dc.type.none.fl_str_mv Articulo
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.folio.none.fl_str_mv 13980001
13980001
dc.identifier.idwos.none.fl_str_mv WOS:000254887100014
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/236904
identifier_str_mv 13980001
WOS:000254887100014
url https://hdl.handle.net/10533/236904
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.1002/jcp.21324
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondap/13980001
dc.relation.uri.none.fl_str_mv https://www.ncbi.nlm.nih.gov/pubmed/18064627
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 3.0 Chile
http://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 3.0 Chile
http://creativecommons.org/licenses/by-nc-nd/3.0/cl/
eu_rights_str_mv openAccess
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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