Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi

Caligus rogercresseyi is a sea louse that affects salmon farming industry throughout the Southern hemisphere. In Chile, oral delivery of emamectin benzoate (EMB) to salmon was the main treatment used; however, C. rogercresseyi became resistant to EMB. The aims of this study were determine the effect...

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Autores: Carcamo-Matus, Juan Guillermo, Aguilar-Pacheco, Marcelo N., Barrientos-Mansilla, Claudia Alejandra, Carreno-Saldias, Constanza Fernanda, Quezada-Monras, Claudia Andrea, Bustos, Carlos, Manriquez-Vasquez, Rene Alfredo, Avendano-Herrera, Ruben Esteban, Yañez-Carcamo, Alejandro Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/196839
Acceso en línea:https://hdl.handle.net/10533/196839
Access Level:acceso abierto
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dc.title.none.fl_str_mv Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi
dc.title.journal.none.fl_str_mv AQUACULTURE
dc.title.journalabbreviation.none.fl_str_mv Aquaculture
title Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi
spellingShingle Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi
Carcamo-Matus, Juan Guillermo
title_short Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi
title_full Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi
title_fullStr Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi
title_full_unstemmed Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi
title_sort Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi
dc.creator.none.fl_str_mv Carcamo-Matus, Juan Guillermo
Aguilar-Pacheco, Marcelo N.
Barrientos-Mansilla, Claudia Alejandra
Carreno-Saldias, Constanza Fernanda
Quezada-Monras, Claudia Andrea
Bustos, Carlos
Manriquez-Vasquez, Rene Alfredo
Avendano-Herrera, Ruben Esteban
Yañez-Carcamo, Alejandro Javier
author Carcamo-Matus, Juan Guillermo
author_facet Carcamo-Matus, Juan Guillermo
Aguilar-Pacheco, Marcelo N.
Barrientos-Mansilla, Claudia Alejandra
Carreno-Saldias, Constanza Fernanda
Quezada-Monras, Claudia Andrea
Bustos, Carlos
Manriquez-Vasquez, Rene Alfredo
Avendano-Herrera, Ruben Esteban
Yañez-Carcamo, Alejandro Javier
author_role author
author2 Aguilar-Pacheco, Marcelo N.
Barrientos-Mansilla, Claudia Alejandra
Carreno-Saldias, Constanza Fernanda
Quezada-Monras, Claudia Andrea
Bustos, Carlos
Manriquez-Vasquez, Rene Alfredo
Avendano-Herrera, Ruben Esteban
Yañez-Carcamo, Alejandro Javier
author2_role author
author
author
author
author
author
author
author
description Caligus rogercresseyi is a sea louse that affects salmon farming industry throughout the Southern hemisphere. In Chile, oral delivery of emamectin benzoate (EMB) to salmon was the main treatment used; however, C. rogercresseyi became resistant to EMB. The aims of this study were determine the effect of EMB on proteins involved in metabolism and drug resistance in rainbow trout (Oncorhynchus mykiss) and C. rogercresseyi. Since no commercial antibodies are available for these salmon's proteins, the expression of trout cytochrome P450 CYP2K1, CYP2M1, CYP3A and CYP3A27, and the multidrug transporters Pgp and MRP1 were investigated by semi-quantitative duplex RT-PCR. Trout specimens infested with C rogercresseyi were medicated with EMB by a standard seven-day oral treatment in salmon farms from southern Chile, and collected between 10 and 25 days after the completion of medication. The samples examined were liver, muscle, gill, middle kidney and intestine from control and EMB treated rainbow trout, and C. rogercresseyi's total homogenates. In trout tissues most CYP and MDR gene expressions were up-regulated by EMB treatment, being MRP1 mRNA in all tissues, but particularly in the kidney and intestine, and CYP2M1 mRNA from muscle and gill, the most augmented. CYP3A, CYP3A27, CYP2K1 and Pgp mRNAs were slightly affected in the most tissues analyzed. In C rogercresseyi minimal up-regulation of the expression for most of mRNA analyzed was observed, except for CYP2M1 mRNA, decreasing its expression by half, and CYP3A27 doubling its expression levels. Nonetheless, CYP3A mRNA expression was not detected in this parasite. These results suggest that treatment with EMB in salmon regulates the transcriptional expression of proteins involved in metabolism, distribution and elimination of endobiotics and xenobiotics, such as hormones and drugs, and even could affect the pharmacokinetics of EMB in the same treatment. (C) 2011 Elsevier B.V. All rights reserved.
publishDate 2011
dc.date.issued.none.fl_str_mv 2011
dc.date.accessioned.none.fl_str_mv 2017-04-27T18:49:22Z
2022-07-07T01:38:44Z
dc.date.available.none.fl_str_mv 2017-04-27T18:49:22Z
2022-07-07T01:38:44Z
dc.type.none.fl_str_mv Articulo
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.eissn.none.fl_str_mv 0
dc.identifier.folio.none.fl_str_mv D08I1096
D08I1096
dc.identifier.idwos.none.fl_str_mv WOS:000297187800008
dc.identifier.isi.none.fl_str_mv WOS:000297187800008
dc.identifier.issn.none.fl_str_mv 0044-8486
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/196839
identifier_str_mv 0
D08I1096
WOS:000297187800008
0044-8486
url https://hdl.handle.net/10533/196839
dc.language.*.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.1016/j.aquaculture.2011.09.012
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondef/D08I1096
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relation.uri.none.fl_str_mv https://doi.org/10.1016/j.aquaculture.2011.09.012
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.spatial.none.fl_str_mv AMSTERDAM
dc.publisher.none.fl_str_mv ELSEVIER SCIENCE BV
publisher.none.fl_str_mv ELSEVIER SCIENCE BV
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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spelling Yañez-Carcamo, Alejandro JavierAvendano-Herrera, Ruben EstebanManriquez-Vasquez, Rene AlfredoBustos, CarlosQuezada-Monras, Claudia AndreaCarreno-Saldias, Constanza FernandaBarrientos-Mansilla, Claudia AlejandraAguilar-Pacheco, Marcelo N.Carcamo-Matus, Juan Guillermo201110.1016/j.aquaculture.2011.09.012https://hdl.handle.net/10533/196839http://purl.org/coar/access_right/c_abf2Effect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyi215207NLDAMSTERDAMCarcamo-Matus, Juan GuillermoAguilar-Pacheco, Marcelo N.Barrientos-Mansilla, Claudia AlejandraCarreno-Saldias, Constanza FernandaQuezada-Monras, Claudia AndreaBustos, CarlosManriquez-Vasquez, Rene AlfredoAvendano-Herrera, Ruben EstebanYañez-Carcamo, Alejandro Javier2017-04-27T18:49:22Z2022-07-07T01:38:44Z2017-04-27T18:49:22Z2022-07-07T01:38:44Z2011Caligus rogercresseyi is a sea louse that affects salmon farming industry throughout the Southern hemisphere. In Chile, oral delivery of emamectin benzoate (EMB) to salmon was the main treatment used; however, C. rogercresseyi became resistant to EMB. The aims of this study were determine the effect of EMB on proteins involved in metabolism and drug resistance in rainbow trout (Oncorhynchus mykiss) and C. rogercresseyi. Since no commercial antibodies are available for these salmon's proteins, the expression of trout cytochrome P450 CYP2K1, CYP2M1, CYP3A and CYP3A27, and the multidrug transporters Pgp and MRP1 were investigated by semi-quantitative duplex RT-PCR. Trout specimens infested with C rogercresseyi were medicated with EMB by a standard seven-day oral treatment in salmon farms from southern Chile, and collected between 10 and 25 days after the completion of medication. The samples examined were liver, muscle, gill, middle kidney and intestine from control and EMB treated rainbow trout, and C. rogercresseyi's total homogenates. In trout tissues most CYP and MDR gene expressions were up-regulated by EMB treatment, being MRP1 mRNA in all tissues, but particularly in the kidney and intestine, and CYP2M1 mRNA from muscle and gill, the most augmented. CYP3A, CYP3A27, CYP2K1 and Pgp mRNAs were slightly affected in the most tissues analyzed. In C rogercresseyi minimal up-regulation of the expression for most of mRNA analyzed was observed, except for CYP2M1 mRNA, decreasing its expression by half, and CYP3A27 doubling its expression levels. Nonetheless, CYP3A mRNA expression was not detected in this parasite. These results suggest that treatment with EMB in salmon regulates the transcriptional expression of proteins involved in metabolism, distribution and elimination of endobiotics and xenobiotics, such as hormones and drugs, and even could affect the pharmacokinetics of EMB in the same treatment. (C) 2011 Elsevier B.V. All rights reserved.This work was supported in part by Grants 1090422 and D08i1096 from the Fondo Nacional de Desarrollo Cientifico and Tecnologico de Chile (FONDECYT) and Fondo de Fomento al Desarrollo Cientifico (FONDEF), respectively; and Direccion de Investigacion y Desarrollo from Universidad Austral de Chile (DID-UACh). We thank the fish farms Trusal SA, Multiexport Foods, Congelados del Pacifico, Biovac, Acuinova Chile, Providencia Fish Farms, Piscicola Nalcahue and Sea Salmon Chile for kindly supplying samples, and also their technical help in the sampling.10FONDEFgcarcamo@uach.clFondo Nacional de Desarrollo Cientifico and Tecnologico de Chile (FONDECYT) [1090422]; Fondo de Fomento al Desarrollo Cientifico (FONDEF) [D08i1096]; Direccion de Investigacion y Desarrollo from Universidad Austral de Chile (DID-UACh)FONDEF0D08I1096D08I1096virtual::30240-1WOS:000297187800008WOS:0002971878000080044-8486https://hdl.handle.net/10533/196839ELSEVIER SCIENCE BVinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI2.010.1016/j.aquaculture.2011.09.012info:eu-repo/grantAgreement/Fondef/D08I1096info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477https://doi.org/10.1016/j.aquaculture.2011.09.012info:eu-repo/semantics/openAccessEffect of emamectin benzoate on transcriptional expression of cytochromes p450 and the multidrug transporters (pgp and mrp1) in rainbow trout (oncorhynchus mykiss) and the sea lice caligus rogercresseyiAQUACULTUREAquacultureArticuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionengArticulohttps://hdl.handle.net/10533/196839FONDEFhttp://purl.org/coar/resource_type/c_2df8fbb1003d8c6a-8a8a-42e2-830b-0a15f3b69ff7virtual::30240-1003d8c6a-8a8a-42e2-830b-0a15f3b69ff7virtual::30240-110533/196839oai:repositorio.anid.cl:10533/1968392023-07-24 17:34:16.726https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
score 15,811543

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