Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile

During the last decades, microbial bioleaching of metallic sulphide ores has become a very important process in the mining industry. The study of biodiversity in those systems has revealed the occurrence of several acidophilic organisms, forming a dynamic community including Bacteria and Archaea. De...

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Autores: Zepeda-Álvarez, Víctor, Demergasso-Semenzato, Cecilia, Galleguillos-Guajardo, Felipe, Ortiz-Riano, Emilio, Castillo, Danny, Guiliani-Guiliani, Nicolas, Galleguillos-Perez, Pedro, Remonsellez-Fuentes, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/198025
Acceso en línea:https://hdl.handle.net/10533/198025
Access Level:acceso abierto
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spelling Remonsellez-Fuentes, FranciscoGalleguillos-Perez, PedroGuiliani-Guiliani, NicolasCastillo, DannyOrtiz-Riano, EmilioGalleguillos-Guajardo, FelipeDemergasso-Semenzato, CeciliaZepeda-Álvarez, Víctor2007https://hdl.handle.net/10533/198025http://purl.org/coar/access_right/c_abf2Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile438435CHESTAFA-ZURICHZepeda-Álvarez, VíctorDemergasso-Semenzato, CeciliaGalleguillos-Guajardo, FelipeOrtiz-Riano, EmilioCastillo, DannyGuiliani-Guiliani, NicolasGalleguillos-Perez, PedroRemonsellez-Fuentes, Francisco2017-04-27T18:54:01Z2022-07-07T02:28:07Z2017-04-27T18:54:01Z2022-07-07T02:28:07Z2007During the last decades, microbial bioleaching of metallic sulphide ores has become a very important process in the mining industry. The study of biodiversity in those systems has revealed the occurrence of several acidophilic organisms, forming a dynamic community including Bacteria and Archaea. Despite the importance of the heap bioleaching process, the metabolic behaviour of a microbial community in bioleaching systems remains unknown. The role played by members of the community inhabiting a bioheap environment has been estimated by the phenotypic characteristics of pure cultures, such as iron oxidation, sulphur oxidation, resistance to high ion concentration and metal tolerance, among others. The global genomic expression of the community inhabiting an industrial bioheap of low-grade copper sulphide ore at Escondida mine in Chile was investigated by random arbitrary primed polymerase chain reaction (RAP-PCR). Three random primers were used and twenty differentially expressed bands were cloned and sequenced. Several sequences were related to 16S rRNA of members of the microbial community; two sequences were related to the ? subunit of pyruvate dehydrogenase from Acidithiobacillus ferrooxidans. The expression levels of the pyruvate dehydrogenase gene in samples from an industrial bioleaching operation at different stages were checked by quantitative real-time PCRFONDEFFONDEF21D04I1169D04I1169virtual::37323-1NO ENCONTRADONO ENCONTRADO1022-6680https://hdl.handle.net/10533/198025TRANS TECH PUBLICATIONS LTDinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI2.0info:eu-repo/grantAgreement/Fondef/D04I1169info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477info:eu-repo/semantics/openAccessDifferential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chileADVANCED MATERIALS RESEARCHADV MAT RES Articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionengArticulohttps://hdl.handle.net/10533/198025FONDEFhttp://purl.org/coar/resource_type/c_2df8fbb117f6ef8c-0a2d-4057-98aa-80ef4e80efd3virtual::37323-117f6ef8c-0a2d-4057-98aa-80ef4e80efd3virtual::37323-110533/198025oai:repositorio.anid.cl:10533/1980252023-07-24 16:28:33.779https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile
dc.title.journal.none.fl_str_mv ADVANCED MATERIALS RESEARCH
dc.title.journalabbreviation.none.fl_str_mv ADV MAT RES 
title Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile
spellingShingle Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile
Zepeda-Álvarez, Víctor
title_short Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile
title_full Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile
title_fullStr Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile
title_full_unstemmed Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile
title_sort Differential genetic expression in heap bioleaching of low grade cooper sulphide ore at escondida mine, chile
dc.creator.none.fl_str_mv Zepeda-Álvarez, Víctor
Demergasso-Semenzato, Cecilia
Galleguillos-Guajardo, Felipe
Ortiz-Riano, Emilio
Castillo, Danny
Guiliani-Guiliani, Nicolas
Galleguillos-Perez, Pedro
Remonsellez-Fuentes, Francisco
author Zepeda-Álvarez, Víctor
author_facet Zepeda-Álvarez, Víctor
Demergasso-Semenzato, Cecilia
Galleguillos-Guajardo, Felipe
Ortiz-Riano, Emilio
Castillo, Danny
Guiliani-Guiliani, Nicolas
Galleguillos-Perez, Pedro
Remonsellez-Fuentes, Francisco
author_role author
author2 Demergasso-Semenzato, Cecilia
Galleguillos-Guajardo, Felipe
Ortiz-Riano, Emilio
Castillo, Danny
Guiliani-Guiliani, Nicolas
Galleguillos-Perez, Pedro
Remonsellez-Fuentes, Francisco
author2_role author
author
author
author
author
author
author
description During the last decades, microbial bioleaching of metallic sulphide ores has become a very important process in the mining industry. The study of biodiversity in those systems has revealed the occurrence of several acidophilic organisms, forming a dynamic community including Bacteria and Archaea. Despite the importance of the heap bioleaching process, the metabolic behaviour of a microbial community in bioleaching systems remains unknown. The role played by members of the community inhabiting a bioheap environment has been estimated by the phenotypic characteristics of pure cultures, such as iron oxidation, sulphur oxidation, resistance to high ion concentration and metal tolerance, among others. The global genomic expression of the community inhabiting an industrial bioheap of low-grade copper sulphide ore at Escondida mine in Chile was investigated by random arbitrary primed polymerase chain reaction (RAP-PCR). Three random primers were used and twenty differentially expressed bands were cloned and sequenced. Several sequences were related to 16S rRNA of members of the microbial community; two sequences were related to the ? subunit of pyruvate dehydrogenase from Acidithiobacillus ferrooxidans. The expression levels of the pyruvate dehydrogenase gene in samples from an industrial bioleaching operation at different stages were checked by quantitative real-time PCR
publishDate 2007
dc.date.issued.none.fl_str_mv 2007
dc.date.accessioned.none.fl_str_mv 2017-04-27T18:54:01Z
2022-07-07T02:28:07Z
dc.date.available.none.fl_str_mv 2017-04-27T18:54:01Z
2022-07-07T02:28:07Z
dc.type.none.fl_str_mv Articulo
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.folio.none.fl_str_mv D04I1169
D04I1169
dc.identifier.idwos.none.fl_str_mv NO ENCONTRADO
dc.identifier.isi.none.fl_str_mv NO ENCONTRADO
dc.identifier.issn.none.fl_str_mv 1022-6680
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/198025
identifier_str_mv D04I1169
NO ENCONTRADO
1022-6680
url https://hdl.handle.net/10533/198025
dc.language.*.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondef/D04I1169
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.spatial.none.fl_str_mv STAFA-ZURICH
dc.publisher.none.fl_str_mv TRANS TECH PUBLICATIONS LTD
publisher.none.fl_str_mv TRANS TECH PUBLICATIONS LTD
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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