DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS

Acidithiobacillus ferrooxidans obtains its energy from the oxidation of ferrous iron, elemental sulfur, or partially oxidized sulfur compounds. The ability of this microorganism to solubilize metal sulfides is successfully applied in biomining operations. Genomic, metagenomic, and high-throughput pr...

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Autores: Beard Borquez, Simon Rodrigo, Chi, An, Hunt, Donald F., Jerez Guevara, Carlos Antonio, Shabanowitz, Jeffrey, Valenzuela Gonzalez, Lissette Andrea
Tipo de recurso: capítulo de libro
Estado:Versión publicada
Fecha de publicación:2008
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/165630
Acceso en línea:https://hdl.handle.net/10533/165630
Access Level:acceso abierto
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spelling Valenzuela Gonzalez, Lissette AndreaShabanowitz, JeffreyJerez Guevara, Carlos AntonioHunt, Donald F.Chi, AnBeard Borquez, Simon Rodrigo200810.1007/978-3-540-72682-1https://hdl.handle.net/10533/165630http://purl.org/coar/access_right/c_abf2DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS8677HOLANDABeard Borquez, Simon RodrigoChi, AnHunt, Donald F.Jerez Guevara, Carlos AntonioShabanowitz, JeffreyValenzuela Gonzalez, Lissette AndreaDahl, ChristianeFriedrich, Cornelius G.2016-12-27T21:50:39Z2022-06-17T20:38:34Z2016-12-27T21:50:39Z2022-06-17T20:38:34Z2008Acidithiobacillus ferrooxidans obtains its energy from the oxidation of ferrous iron, elemental sulfur, or partially oxidized sulfur compounds. The ability of this microorganism to solubilize metal sulfides is successfully applied in biomining operations. Genomic, metagenomic, and high-throughput proteomic studies of the global regulatory responses that biomining microorganisms use to adapt to their changing environment are just beginning to emerge. To further study some of the components involved in sulfur metabolism, differential expression proteomics of total periplasmic proteins was done by high-resolution LTQ FT ion trap mass spectrometry. Of 216 proteins found in the periplasm, several of them changed their levels of synthesis during growth of A. ferrooxidans ATCC 23270 in thiosulfate, elemental sulfur and ferrous iron. Thirty-four percent of them corresponded to unknown proteins. Forty-one proteins were exclusively present in sulfur-grown cells and 14 in thiosulfate-grown cells. The putative genes coding for these proteins were localized in the available genomic sequence of A. ferrooxidans ATCC 23270. The genomic context around several of these genes suggests their involvement in sulfur metabolism and possibly in sulfur oxidation and formation of FeS clusters. Many of the periplasmic proteins changing their expression during growth in sulfur compounds may have important roles yet to be described in the sulfur metabolism of this acidophilic microorganism. This knowledge will eventually help to improve mineral bioleaching processes.FONDECYT303FONDECYT1030767978-3-540-72679-1978-3-642-09173-5https://hdl.handle.net/10533/165630engSPRINGERinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI 2.010.1007/978-3-540-72682-1info:eu-repo/grantAgreement/Fondecyt/1030767info:eu-repo/semantics/dataset/hdl.handle.net/10533/93479info:eu-repo/semantics/openAccessDIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANSMICROBIAL SULFUR METABOLISMCapitulo de libroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPartCapituloLibrohttps://hdl.handle.net/10533/165630FONDECYThttp://purl.org/coar/resource_type/c_3248f110bae2-9d3e-46a5-aaa0-57819e341434virtual::13114-1f110bae2-9d3e-46a5-aaa0-57819e341434virtual::13114-110533/165630oai:repositorio.anid.cl:10533/1656302023-07-04 00:10:10.877https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS
dc.title.libro.none.fl_str_mv MICROBIAL SULFUR METABOLISM
title DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS
spellingShingle DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS
Beard Borquez, Simon Rodrigo
title_short DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS
title_full DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS
title_fullStr DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS
title_full_unstemmed DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS
title_sort DIFFERENTIAL EXPRESSION PROTEOMICS FOR THE STUDY OF SULFUR METABOLISM IN THE CHEMOLITHOAUTOTROPHIC ACIDITHIOBACILLUS FERROOXIDANS
dc.creator.none.fl_str_mv Beard Borquez, Simon Rodrigo
Chi, An
Hunt, Donald F.
Jerez Guevara, Carlos Antonio
Shabanowitz, Jeffrey
Valenzuela Gonzalez, Lissette Andrea
dc.creator.libro.none.fl_str_mv Dahl, Christiane
Friedrich, Cornelius G.
author Beard Borquez, Simon Rodrigo
author_facet Beard Borquez, Simon Rodrigo
Chi, An
Hunt, Donald F.
Jerez Guevara, Carlos Antonio
Shabanowitz, Jeffrey
Valenzuela Gonzalez, Lissette Andrea
author_role author
author2 Chi, An
Hunt, Donald F.
Jerez Guevara, Carlos Antonio
Shabanowitz, Jeffrey
Valenzuela Gonzalez, Lissette Andrea
author2_role author
author
author
author
author
description Acidithiobacillus ferrooxidans obtains its energy from the oxidation of ferrous iron, elemental sulfur, or partially oxidized sulfur compounds. The ability of this microorganism to solubilize metal sulfides is successfully applied in biomining operations. Genomic, metagenomic, and high-throughput proteomic studies of the global regulatory responses that biomining microorganisms use to adapt to their changing environment are just beginning to emerge. To further study some of the components involved in sulfur metabolism, differential expression proteomics of total periplasmic proteins was done by high-resolution LTQ FT ion trap mass spectrometry. Of 216 proteins found in the periplasm, several of them changed their levels of synthesis during growth of A. ferrooxidans ATCC 23270 in thiosulfate, elemental sulfur and ferrous iron. Thirty-four percent of them corresponded to unknown proteins. Forty-one proteins were exclusively present in sulfur-grown cells and 14 in thiosulfate-grown cells. The putative genes coding for these proteins were localized in the available genomic sequence of A. ferrooxidans ATCC 23270. The genomic context around several of these genes suggests their involvement in sulfur metabolism and possibly in sulfur oxidation and formation of FeS clusters. Many of the periplasmic proteins changing their expression during growth in sulfur compounds may have important roles yet to be described in the sulfur metabolism of this acidophilic microorganism. This knowledge will eventually help to improve mineral bioleaching processes.
publishDate 2008
dc.date.issued.none.fl_str_mv 2008
dc.date.accessioned.none.fl_str_mv 2016-12-27T21:50:39Z
2022-06-17T20:38:34Z
dc.date.available.none.fl_str_mv 2016-12-27T21:50:39Z
2022-06-17T20:38:34Z
dc.type.none.fl_str_mv Capitulo de libro
info:eu-repo/semantics/publishedVersion
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.folio.none.fl_str_mv 1030767
dc.identifier.isbn.none.fl_str_mv 978-3-540-72679-1
978-3-642-09173-5
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/165630
identifier_str_mv 1030767
978-3-540-72679-1
978-3-642-09173-5
url https://hdl.handle.net/10533/165630
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
instname: Conicyt
reponame: Repositorio Digital RI 2.0
dc.relation.doi.none.fl_str_mv 10.1007/978-3-540-72682-1
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondecyt/1030767
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93479
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.editorial.none.fl_str_mv SPRINGER
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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score 15.811543

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