Participation of the nadph oxidase complex and p38 mapk in the production of cellular ros and in the sumoilation of the viral nucleoprotein, essential for the maturation and production of the viral progenie of isav
Infectious salmon anemia virus (ISAv) is a pathogen capable of causing a serious clinical disease called infectious salmon anemia (ISA). Controlling this pathology poses a huge challenge for global and national salmon farming, considering that the mortality rate of infected salmon is around 50%, whi...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | Chile |
| OAI Identifier: | oai:repositorio.anid.cl:10533/253034 |
| Acceso en línea: | https://hdl.handle.net/10533/253034 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciencias Naturales Otras Ciencias Naturales |
| Sumario: | Infectious salmon anemia virus (ISAv) is a pathogen capable of causing a serious clinical disease called infectious salmon anemia (ISA). Controlling this pathology poses a huge challenge for global and national salmon farming, considering that the mortality rate of infected salmon is around 50%, which translates into considerable economic losses for the industry. Previously, it has been studied that this virus is capable of producing strong oxidative stress caused by the activation of p38 MAPK. The activity of this protein induces the over-activation of the NADPH oxidase complex, a phenomenon that generates a redox imbalance at the cellular level due to the increased production of superoxide ion and various reactive oxygen species derived from it. This redox alteration generated by the viral particle could be necessary for the replication and maturation of the virion. On the other hand, viruses have been shown in various infection models to be able to use the SUMOylation mechanisms of the host cell to SUMOylate their own proteins, as part of their strategy to achieve the spread of infectious viral particles. In this context, SUMOylation studies have been carried out on cells infected with the Influenza virus, belonging to the same ISAv family, where it has been shown that the virus requires this post-translational process to modify its own proteins, as it is with the NP protein, which is essential for the assembly of the viral particle in the nucleus to occur and thus an increase in the viral progeny. These findings allow us to suppose that the same phenomenon would be occurring in an infection with ISAv and it is novel to relate the oxidative stress generated by this pathogen with changes in the degree of SUMOylation of its viral proteins. The comprehensive analysis of the results showed that the oxidative stress produced by an increase in NADPH oxidase through the increase in p38 MAPK induces a significant SUMOylation of the NP protein of ISAv, which would be allowing for a greater viral replication and therefore a greater production in viral progeny, it was also seen that the inhibition of oxidative stress by a berrie such as maqui causes a strong decrease in the NP SUMOylation, increasing the ubiquitination of this protein and as a consequence a decrease in viral progeny. Therefore, maqui could be considered a functional food for the salmon in the future, thus reducing the risks of mortality caused by an infection with ISAv since it is capable of inducing the inhibition of viral progeny. |
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