Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis

The Salmon Rickettsia syndrome (SRS) remains a major infectious disease in the Chilean aquaculture. A limited number of Piscirickettsia salmonis proteins have been characterized so far for their use as potential candidates for vaccines studies. In this study, we identified and expressed a highly imm...

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Autores: MARSHALL-GONZALEZ, SERGIO, HENRIQUEZ-QUEZADA, VITALIA, OLIVARES-PACHECO, JORGE, CATALDO-TAMAYO, PATRICIO, CONEJEROS, PABLO, GOMEZ, FERNANDO, ZAHR, MARCELA
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/197982
Acceso en línea:https://hdl.handle.net/10533/197982
Access Level:acceso abierto
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spelling ZAHR, MARCELAGOMEZ, FERNANDOCONEJEROS, PABLOCATALDO-TAMAYO, PATRICIOOLIVARES-PACHECO, JORGEHENRIQUEZ-QUEZADA, VITALIAMARSHALL-GONZALEZ, SERGIO200710.1016/j.vaccine.2006.11.035https://hdl.handle.net/10533/197982http://purl.org/coar/access_right/c_abf2Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis21022095GBROXFORDMARSHALL-GONZALEZ, SERGIOHENRIQUEZ-QUEZADA, VITALIAOLIVARES-PACHECO, JORGECATALDO-TAMAYO, PATRICIOCONEJEROS, PABLOGOMEZ, FERNANDOZAHR, MARCELA2017-04-27T18:53:48Z2022-07-07T02:27:31Z2017-04-27T18:53:48Z2022-07-07T02:27:31Z2007The Salmon Rickettsia syndrome (SRS) remains a major infectious disease in the Chilean aquaculture. A limited number of Piscirickettsia salmonis proteins have been characterized so far for their use as potential candidates for vaccines studies. In this study, we identified and expressed a highly immunogenic protein of P. salmonis extracted by selective hydrophobicity from crude-cell macerates of naturally infected salmonid fish. One and two-D PAGE gels followed by Western blot analysis with a battery of polyclonal anti-P. salmonis antibodies have allowed the isolation of the target protein. Basic local alignment search (BLAST) done after partial sequencing of the pure protein identified it as a member of the heat-shock protein (HSP) family of prokaryotes. The protein, named ChaPs, was cloned as a single open reading frame encoding 545 amino acid residues with a predicted molecular mass of 57.3 kDa. The amplicon representing the entire novel gene was expressed in vitro in different heterologous systems: the PurePro Caulobacter crescentus expression system from where most of the characterization was attained, and also in the Escherichia coli BL-21 CodonPlus model for commercially potential purposes. The immunologic potential of ChaPs was determined with serum from naturally infected fish. (c) 2006 Elsevier Ltd. All rights reserved.017FONDEFsmarshal@ucv.cl011FONDEF250D03I1137D03I1137virtual::23988-1WOS:000244948100019WOS:0002449481000190264-410Xhttps://hdl.handle.net/10533/197982ELSEVIER SCI LTDinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI2.010.1016/j.vaccine.2006.11.035info:eu-repo/grantAgreement/Fondef/D03I1137info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477https://doi.org/10.1016/j.vaccine.2006.11.035info:eu-repo/semantics/openAccessImmunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonisVACCINEVaccineArticuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionengArticulohttps://hdl.handle.net/10533/197982FONDEFhttp://purl.org/coar/resource_type/c_2df8fbb1bf711a23-ce46-42cc-8c6f-df978f4a1a55virtual::23988-1bf711a23-ce46-42cc-8c6f-df978f4a1a55virtual::23988-110533/197982oai:repositorio.anid.cl:10533/1979822023-07-24 18:46:52.922https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis
dc.title.journal.none.fl_str_mv VACCINE
dc.title.journalabbreviation.none.fl_str_mv Vaccine
title Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis
spellingShingle Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis
MARSHALL-GONZALEZ, SERGIO
title_short Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis
title_full Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis
title_fullStr Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis
title_full_unstemmed Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis
title_sort Immunological characterization of a bacterial protein isolated from salmonid fish naturally infected with piscirickettsia salmonis
dc.creator.none.fl_str_mv MARSHALL-GONZALEZ, SERGIO
HENRIQUEZ-QUEZADA, VITALIA
OLIVARES-PACHECO, JORGE
CATALDO-TAMAYO, PATRICIO
CONEJEROS, PABLO
GOMEZ, FERNANDO
ZAHR, MARCELA
author MARSHALL-GONZALEZ, SERGIO
author_facet MARSHALL-GONZALEZ, SERGIO
HENRIQUEZ-QUEZADA, VITALIA
OLIVARES-PACHECO, JORGE
CATALDO-TAMAYO, PATRICIO
CONEJEROS, PABLO
GOMEZ, FERNANDO
ZAHR, MARCELA
author_role author
author2 HENRIQUEZ-QUEZADA, VITALIA
OLIVARES-PACHECO, JORGE
CATALDO-TAMAYO, PATRICIO
CONEJEROS, PABLO
GOMEZ, FERNANDO
ZAHR, MARCELA
author2_role author
author
author
author
author
author
description The Salmon Rickettsia syndrome (SRS) remains a major infectious disease in the Chilean aquaculture. A limited number of Piscirickettsia salmonis proteins have been characterized so far for their use as potential candidates for vaccines studies. In this study, we identified and expressed a highly immunogenic protein of P. salmonis extracted by selective hydrophobicity from crude-cell macerates of naturally infected salmonid fish. One and two-D PAGE gels followed by Western blot analysis with a battery of polyclonal anti-P. salmonis antibodies have allowed the isolation of the target protein. Basic local alignment search (BLAST) done after partial sequencing of the pure protein identified it as a member of the heat-shock protein (HSP) family of prokaryotes. The protein, named ChaPs, was cloned as a single open reading frame encoding 545 amino acid residues with a predicted molecular mass of 57.3 kDa. The amplicon representing the entire novel gene was expressed in vitro in different heterologous systems: the PurePro Caulobacter crescentus expression system from where most of the characterization was attained, and also in the Escherichia coli BL-21 CodonPlus model for commercially potential purposes. The immunologic potential of ChaPs was determined with serum from naturally infected fish. (c) 2006 Elsevier Ltd. All rights reserved.
publishDate 2007
dc.date.issued.none.fl_str_mv 2007
dc.date.accessioned.none.fl_str_mv 2017-04-27T18:53:48Z
2022-07-07T02:27:31Z
dc.date.available.none.fl_str_mv 2017-04-27T18:53:48Z
2022-07-07T02:27:31Z
dc.type.none.fl_str_mv Articulo
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.eissn.none.fl_str_mv 0
dc.identifier.folio.none.fl_str_mv D03I1137
D03I1137
dc.identifier.idwos.none.fl_str_mv WOS:000244948100019
dc.identifier.isi.none.fl_str_mv WOS:000244948100019
dc.identifier.issn.none.fl_str_mv 0264-410X
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/197982
identifier_str_mv 0
D03I1137
WOS:000244948100019
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url https://hdl.handle.net/10533/197982
dc.language.*.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.1016/j.vaccine.2006.11.035
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondef/D03I1137
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relation.uri.none.fl_str_mv https://doi.org/10.1016/j.vaccine.2006.11.035
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.spatial.none.fl_str_mv OXFORD
dc.publisher.none.fl_str_mv ELSEVIER SCI LTD
publisher.none.fl_str_mv ELSEVIER SCI LTD
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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