Identification and characterization of the biosynthesis, perception and signaling pathway of brassinosteroids during the development of Vitis vinifera L.
The grapevine (Vitis vinifera L.) is one of the most cultivated plant species in the world. Within the agronomic practices for its cultivation and improvement are pruning and the use of rootstocks and phytohormones. In recent years, interest has arisen in the study of brassinosteroids (BRs) as a gro...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | Chile |
| OAI Identifier: | oai:repositorio.anid.cl:10533/246244 |
| Acceso en línea: | https://hdl.handle.net/10533/246244 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciencias Naturales Otras Ciencias Naturales |
| Sumario: | The grapevine (Vitis vinifera L.) is one of the most cultivated plant species in the world. Within the agronomic practices for its cultivation and improvement are pruning and the use of rootstocks and phytohormones. In recent years, interest has arisen in the study of brassinosteroids (BRs) as a growth regulator to improve productive parameters in grapevine berries. BRs are a family of sterol-derived phytohormones that regulate physiological processes such as responses to biotic and abiotic stress, senescence control, growth promotion through cell division and expansion, among others. So far, only the VvBR6OX1 gene of the biosynthesis pathway has been characterized. In this thesis, a differential gene expression profile of genes involved in biosynthesis, perception, and signaling of BRs depending on the tissue and development stage was determined. Besides, ten different types of BRs were quantified in grapevine tissues. To understand the role in growth, exogenous applications of this hormone were made in berries, in which the changes in metabolites and transcripts were analyzed. Finally, the VvBIN2.1 gene was characterized to demonstrate its role in growth using A. thaliana as a model. This work represents the broadest characterization of BRs in grapevines carried out to date. It provides a series of antecedents that could allow the future incorporation of this hormone in agronomic management plans. |
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