Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels

Brain ischemia causes more extensive injury in hyperglycemic than normoglycemic subjects, and the increased damage is to astroglia as well as neurons. In the present work, we found that in cortical astrocytes from rat or mouse, reoxygenation after hypoxia in a medium mimicking interstitial fluid dur...

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Autores: Velarde-Aliaga, María, Saez-Carreño, Juan, Bennet, Michael, Ezan, Pascal, Giaume, Christian, Hernández, Diego, Orellana, Juan
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/197920
Acesso em linha:https://hdl.handle.net/10533/197920
Access Level:acceso abierto
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spelling Orellana, JuanHernández, DiegoGiaume, ChristianEzan, PascalBennet, MichaelSaez-Carreño, JuanVelarde-Aliaga, María201010.1002/glia.20926https://hdl.handle.net/10533/197920http://purl.org/coar/access_right/c_abf2Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels343329USAHOBOKENVelarde-Aliaga, MaríaSaez-Carreño, JuanBennet, MichaelEzan, PascalGiaume, ChristianHernández, DiegoOrellana, Juan2017-04-27T18:53:33Z2022-07-07T02:26:46Z2017-04-27T18:53:33Z2022-07-07T02:26:46Z2010Brain ischemia causes more extensive injury in hyperglycemic than normoglycemic subjects, and the increased damage is to astroglia as well as neurons. In the present work, we found that in cortical astrocytes from rat or mouse, reoxygenation after hypoxia in a medium mimicking interstitial fluid during ischemia increases hemichannel activity and decreases cell-cell communication via gap junctions as indicated by dye uptake and dye coupling, respectively. These effects were potentiated by high glucose during the hypoxia in a concentration-dependent manner (and by zero glucose) and were not observed in connexin 43(-/-) astrocytes. The responses were transient and persistent after short and long periods of hypoxia, respectively. The persistent responses were associated with a progressive reduction in cell viability that was prevented by La(3+) or peptides that block connexin 43 (Cx43) hemichannels or by inhibition of p38 MAP kinase prior to hypoxia-reoxygenation but not by treatments that block pannexin hemichannels. Block of Cx43 hemichannels did not affect the reduction in gap junction mediated dye coupling observed during reoxygenation. Cx43 hemichannels may be a novel therapeutic target to reduce cell death following stroke, particularly in hyperglycemic conditions. (C) 2009 Wiley-Liss, Inc.CONICYT; Grant number: 24080055 (to J.A.O.); Grant sponsor: FONDECYT; Grant number: 1070591 (to J.C.S.); Grant sponsor: FONDEF; Grant number: D0711086 (to J.C.S.); Grant sponsor: NIH; Grant numbers: NS37402, NS45287 (to M.V.L.B.); Grant sponsor: INSERM/CONICYT (to C.G. and J.C.S.).66FONDEFjaorella@uc.clCONICYT [24080055]; FONDECYT [1070591]; FONDEF [D0711086]; NIH [NS37402, NS45287]; INSERM/CONICYT3FONDEF580D07I1086D07I1086virtual::42691-1WOS:000273189600007WOS:0002731896000070894-1491https://hdl.handle.net/10533/197920WILEY-LISSinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI2.010.1002/glia.20926info:eu-repo/grantAgreement/Fondef/D07I1086info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477https://doi.org/10.1002/glia.20926info:eu-repo/semantics/openAccessHypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannelsGLIAGliaArticuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionengArticulohttps://hdl.handle.net/10533/197920FONDEFhttp://purl.org/coar/resource_type/c_2df8fbb1db41e1be-ddc8-49a6-82fc-ac8c925a39b7virtual::42691-1db41e1be-ddc8-49a6-82fc-ac8c925a39b7virtual::42691-110533/197920oai:repositorio.anid.cl:10533/1979202023-07-24 17:29:27.787https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
dc.title.journal.none.fl_str_mv GLIA
dc.title.journalabbreviation.none.fl_str_mv Glia
title Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
spellingShingle Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
Velarde-Aliaga, María
title_short Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
title_full Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
title_fullStr Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
title_full_unstemmed Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
title_sort Hypoxia in high glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through increased permeability of connexin 43 hemichannels
dc.creator.none.fl_str_mv Velarde-Aliaga, María
Saez-Carreño, Juan
Bennet, Michael
Ezan, Pascal
Giaume, Christian
Hernández, Diego
Orellana, Juan
author Velarde-Aliaga, María
author_facet Velarde-Aliaga, María
Saez-Carreño, Juan
Bennet, Michael
Ezan, Pascal
Giaume, Christian
Hernández, Diego
Orellana, Juan
author_role author
author2 Saez-Carreño, Juan
Bennet, Michael
Ezan, Pascal
Giaume, Christian
Hernández, Diego
Orellana, Juan
author2_role author
author
author
author
author
author
description Brain ischemia causes more extensive injury in hyperglycemic than normoglycemic subjects, and the increased damage is to astroglia as well as neurons. In the present work, we found that in cortical astrocytes from rat or mouse, reoxygenation after hypoxia in a medium mimicking interstitial fluid during ischemia increases hemichannel activity and decreases cell-cell communication via gap junctions as indicated by dye uptake and dye coupling, respectively. These effects were potentiated by high glucose during the hypoxia in a concentration-dependent manner (and by zero glucose) and were not observed in connexin 43(-/-) astrocytes. The responses were transient and persistent after short and long periods of hypoxia, respectively. The persistent responses were associated with a progressive reduction in cell viability that was prevented by La(3+) or peptides that block connexin 43 (Cx43) hemichannels or by inhibition of p38 MAP kinase prior to hypoxia-reoxygenation but not by treatments that block pannexin hemichannels. Block of Cx43 hemichannels did not affect the reduction in gap junction mediated dye coupling observed during reoxygenation. Cx43 hemichannels may be a novel therapeutic target to reduce cell death following stroke, particularly in hyperglycemic conditions. (C) 2009 Wiley-Liss, Inc.
publishDate 2010
dc.date.issued.none.fl_str_mv 2010
dc.date.accessioned.none.fl_str_mv 2017-04-27T18:53:33Z
2022-07-07T02:26:46Z
dc.date.available.none.fl_str_mv 2017-04-27T18:53:33Z
2022-07-07T02:26:46Z
dc.type.none.fl_str_mv Articulo
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dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.eissn.none.fl_str_mv 0
dc.identifier.folio.none.fl_str_mv D07I1086
D07I1086
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dc.identifier.isi.none.fl_str_mv WOS:000273189600007
dc.identifier.issn.none.fl_str_mv 0894-1491
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dc.language.*.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.1002/glia.20926
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondef/D07I1086
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relation.uri.none.fl_str_mv https://doi.org/10.1002/glia.20926
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv WILEY-LISS
publisher.none.fl_str_mv WILEY-LISS
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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