La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental

Objective Background. Over expression of the gene for homologous angiotensin I converting enzyme (ACE) is associated with prevention of angiotensin II dependent cardiac hypertrophy and fibrosis. Whether this over expression is able to reduce hypertension and revert cardiac remodeling is unknown. Aim...

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Autores: Ramos, E., Herrera, A., Moya, J., Apablaza, F., Godoy, I., Jalil, J., Lavandero, S., Chiong, M., Ocaranza, M. P.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/242068
Acceso en línea:https://hdl.handle.net/10533/242068
Access Level:acceso abierto
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spelling Ocaranza, M. P.Chiong, M.Lavandero, S.Jalil, J.Godoy, I.Apablaza, F.Moya, J.Herrera, A.Ramos, E.201010.4067/S0718-85602010000300008https://hdl.handle.net/10533/242068http://purl.org/coar/access_right/c_abf2La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimentalRamos, E.Herrera, A.Moya, J.Apablaza, F.Godoy, I.Jalil, J.Lavandero, S.Chiong, M.Ocaranza, M. P.2020-07-09T21:15:24Z2022-07-08T20:15:31Z2020-07-09T21:15:24Z2022-07-08T20:15:31Z2010Objective Background. Over expression of the gene for homologous angiotensin I converting enzyme (ACE) is associated with prevention of angiotensin II dependent cardiac hypertrophy and fibrosis. Whether this over expression is able to reduce hypertension and revert cardiac remodeling is unknown. Aim: To determine whether adenoviral ACE2 gene over expression in the myocardium decreases experimental hypertension and cardiac remodeling in rats with genetically determined levéis of ACE and ANGII. Methods: Homozygous normotensive Lewis (LL) and Brown Norway (BN) rats with decreased or increased levéis of ACE and Ang II, respectively, were made hy-pertensive using the Goldblatt procedure. Sham opera-ted rats were used as control s. 5 weeks after surgery, when systolic blood pressure reached > 140 mmHg, rats were randomized to receive intramyocardial injec-tion of either AdACE2 or green fluorescent Ad protein (GFP) as infection controlling agents. One week after adenoviral infection, rats were sacrificed. Body weight (BW, Gr), relative cardiac mass (RCM, mG), systolic blood pressure (SBP, mmHg), cardiomyocite área (MA um2) and perimeter (MPER, uM), and myocardial co-llagen content, both subendocardial (SC, %) and total (TC %) were measured. Results: Hypertension was associated to significant in-creases in total and RCM, MA, MPER as well as SC and TC in Goldblatt vs normal rats. This was indepen-dent of BW and not affected by ACE polymorphism. ACE II over expression significantly decreased SBP (27 vs 15%), MA (25 vs 25%) and MPER (18 vs 17%) hy-pertensive vs normal rats, respectively. Conclusión: Over expression of ACE2 decreased hypertension, hypertrophy and fibrosis in rats with experimental hypertension and different levéis of ACE and Ang II. (Fondecyt 1070662).CEMCFONDAPFONDAP1501000615010006virtual::19271-1no isihttps://hdl.handle.net/10533/242068enginstname: ANIDreponame: Repositorio Digital RI2.010.4067/S0718-85602010000300008https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-85602010000300008info:eu-repo/semantics/openAccessLa sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimentalRevista Chilena de CardiologíaArticuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttps://hdl.handle.net/10533/242068FONDAPhttp://purl.org/coar/resource_type/c_2df8fbb1f93d863d-c170-4dfb-af15-3dcaf3e91b3dvirtual::19271-1f93d863d-c170-4dfb-af15-3dcaf3e91b3dvirtual::19271-110533/242068oai:repositorio.anid.cl:10533/2420682023-07-24 18:07:33.592https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
dc.title.journal.none.fl_str_mv Revista Chilena de Cardiología
title La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
spellingShingle La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
Ramos, E.
title_short La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
title_full La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
title_fullStr La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
title_full_unstemmed La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
title_sort La sobreexpresión del gen de enzina convertidora de angiotensina homóloga (ECA2) revierte la hipertensión arterial y el remodelado cardíaco experimental
dc.creator.none.fl_str_mv Ramos, E.
Herrera, A.
Moya, J.
Apablaza, F.
Godoy, I.
Jalil, J.
Lavandero, S.
Chiong, M.
Ocaranza, M. P.
author Ramos, E.
author_facet Ramos, E.
Herrera, A.
Moya, J.
Apablaza, F.
Godoy, I.
Jalil, J.
Lavandero, S.
Chiong, M.
Ocaranza, M. P.
author_role author
author2 Herrera, A.
Moya, J.
Apablaza, F.
Godoy, I.
Jalil, J.
Lavandero, S.
Chiong, M.
Ocaranza, M. P.
author2_role author
author
author
author
author
author
author
author
description Objective Background. Over expression of the gene for homologous angiotensin I converting enzyme (ACE) is associated with prevention of angiotensin II dependent cardiac hypertrophy and fibrosis. Whether this over expression is able to reduce hypertension and revert cardiac remodeling is unknown. Aim: To determine whether adenoviral ACE2 gene over expression in the myocardium decreases experimental hypertension and cardiac remodeling in rats with genetically determined levéis of ACE and ANGII. Methods: Homozygous normotensive Lewis (LL) and Brown Norway (BN) rats with decreased or increased levéis of ACE and Ang II, respectively, were made hy-pertensive using the Goldblatt procedure. Sham opera-ted rats were used as control s. 5 weeks after surgery, when systolic blood pressure reached > 140 mmHg, rats were randomized to receive intramyocardial injec-tion of either AdACE2 or green fluorescent Ad protein (GFP) as infection controlling agents. One week after adenoviral infection, rats were sacrificed. Body weight (BW, Gr), relative cardiac mass (RCM, mG), systolic blood pressure (SBP, mmHg), cardiomyocite área (MA um2) and perimeter (MPER, uM), and myocardial co-llagen content, both subendocardial (SC, %) and total (TC %) were measured. Results: Hypertension was associated to significant in-creases in total and RCM, MA, MPER as well as SC and TC in Goldblatt vs normal rats. This was indepen-dent of BW and not affected by ACE polymorphism. ACE II over expression significantly decreased SBP (27 vs 15%), MA (25 vs 25%) and MPER (18 vs 17%) hy-pertensive vs normal rats, respectively. Conclusión: Over expression of ACE2 decreased hypertension, hypertrophy and fibrosis in rats with experimental hypertension and different levéis of ACE and Ang II. (Fondecyt 1070662).
publishDate 2010
dc.date.issued.none.fl_str_mv 2010
dc.date.accessioned.none.fl_str_mv 2020-07-09T21:15:24Z
2022-07-08T20:15:31Z
dc.date.available.none.fl_str_mv 2020-07-09T21:15:24Z
2022-07-08T20:15:31Z
dc.type.none.fl_str_mv Articulo
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.folio.none.fl_str_mv 15010006
15010006
dc.identifier.idwos.none.fl_str_mv no isi
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/242068
identifier_str_mv 15010006
no isi
url https://hdl.handle.net/10533/242068
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: ANID
reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.4067/S0718-85602010000300008
dc.relation.uri.none.fl_str_mv https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-85602010000300008
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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score 15,811543

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