Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing

In an attempt to establish a protocol for the cryopreservation of the spermatozoa of the rainbow trout (Oncorhynchus mykiss), we studied the effect of various cryoprotective agents (CA) in spermatozoa motility and viability, before, during, and after freezing. Freezing was performed by using a contr...

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Autores: CONGET, PAULETTE, FERNANDEZ, MIREYA, HERRERA, GUILLERMO, MINGUELL, JOSE
Formato: artículo
Estado:Versión publicada
Fecha de publicación:1996
País:Chile
Idioma:inglés
OAI Identifier:oai:repositorio.anid.cl:10533/197734
Acesso em linha:https://hdl.handle.net/10533/197734
Access Level:acceso abierto
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spelling MINGUELL, JOSEHERRERA, GUILLERMOFERNANDEZ, MIREYACONGET, PAULETTE199610.1016/0044-8486(96)01275-6https://hdl.handle.net/10533/197734http://purl.org/coar/access_right/c_abf2Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing329319NLDAMSTERDAMCONGET, PAULETTEFERNANDEZ, MIREYAHERRERA, GUILLERMOMINGUELL, JOSE2017-04-27T18:52:41Z2022-07-07T02:24:34Z2017-04-27T18:52:41Z2022-07-07T02:24:34Z1996In an attempt to establish a protocol for the cryopreservation of the spermatozoa of the rainbow trout (Oncorhynchus mykiss), we studied the effect of various cryoprotective agents (CA) in spermatozoa motility and viability, before, during, and after freezing. Freezing was performed by using a controlled rate freezing system, which allows the accurate setting of different cooling rates, as well as a proper recording of intra-sample temperatures throughout the procedure. Results obtained indicate that before the initiation of freezing, spermatozoa motility is affected more by the length of time of exposure to CA than by the chemical nature of the agents. Exposure periods longer than 10 min affected motility irreversibly, which seems to be related to the high osmolarity of the extender solutions, To study the changes in spermatozoa motility and viability during and after cryopreservation, cells in the cryoprotective solution (glycerol, DMSO or DMSO-sucrose) were processed in a programmable biological freezer at slow (1 degrees C and 10 degrees C min(-1)) or rapid (30 degrees C min(-1)) cooling rates. Results obtained indicated that both during (-60 degrees C) and after completion of the freezing program (-80 degrees C, followed by storage under liquid nitrogen), motility and viability was well preserved only in the cells in DMSO-sucrose when subjected to a rapid cooling rate. Under these conditions, approximately 63% of spermatozoa were alive and showed progressive motility, Together, the average fertilization potential of cryopreserved semen was 58% (47% to 85%) compared with that of fresh semen. The procedure described here provides consistency and precision, and permits processing, freezing and storage of trout spermatozoa in less than 15 min.038FONDEF00FONDEF1430D91I1140D91I1140virtual::45840-1WOS:A1996VH50000009WOS:A1996VH500000090044-8486https://hdl.handle.net/10533/197734ELSEVIER SCIENCE BVinstname: Conicytreponame: Repositorio Digital RI2.0instname: Conicytreponame: Repositorio Digital RI2.010.1016/0044-8486(96)01275-6info:eu-repo/grantAgreement/Fondef/D91I1140info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477https://doi.org/10.1016/0044-8486(96)01275-6info:eu-repo/semantics/openAccessCryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezingAQUACULTUREAquacultureArticuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionengArticulohttps://hdl.handle.net/10533/197734FONDEFhttp://purl.org/coar/resource_type/c_2df8fbb14fe4cc4e-526a-4ff9-bd20-64b60eb6f721virtual::45840-14fe4cc4e-526a-4ff9-bd20-64b60eb6f721virtual::45840-110533/197734oai:repositorio.anid.cl:10533/1977342023-07-24 18:02:17.26https://repositorio.anid.clRepositorio ANIDaletelier@anid.cl
dc.title.none.fl_str_mv Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing
dc.title.journal.none.fl_str_mv AQUACULTURE
dc.title.journalabbreviation.none.fl_str_mv Aquaculture
title Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing
spellingShingle Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing
CONGET, PAULETTE
title_short Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing
title_full Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing
title_fullStr Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing
title_full_unstemmed Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing
title_sort Cryopreservation of rainbow trout (oncorhynchus mykiss)spermatozoa using programmable freezing
dc.creator.none.fl_str_mv CONGET, PAULETTE
FERNANDEZ, MIREYA
HERRERA, GUILLERMO
MINGUELL, JOSE
author CONGET, PAULETTE
author_facet CONGET, PAULETTE
FERNANDEZ, MIREYA
HERRERA, GUILLERMO
MINGUELL, JOSE
author_role author
author2 FERNANDEZ, MIREYA
HERRERA, GUILLERMO
MINGUELL, JOSE
author2_role author
author
author
description In an attempt to establish a protocol for the cryopreservation of the spermatozoa of the rainbow trout (Oncorhynchus mykiss), we studied the effect of various cryoprotective agents (CA) in spermatozoa motility and viability, before, during, and after freezing. Freezing was performed by using a controlled rate freezing system, which allows the accurate setting of different cooling rates, as well as a proper recording of intra-sample temperatures throughout the procedure. Results obtained indicate that before the initiation of freezing, spermatozoa motility is affected more by the length of time of exposure to CA than by the chemical nature of the agents. Exposure periods longer than 10 min affected motility irreversibly, which seems to be related to the high osmolarity of the extender solutions, To study the changes in spermatozoa motility and viability during and after cryopreservation, cells in the cryoprotective solution (glycerol, DMSO or DMSO-sucrose) were processed in a programmable biological freezer at slow (1 degrees C and 10 degrees C min(-1)) or rapid (30 degrees C min(-1)) cooling rates. Results obtained indicated that both during (-60 degrees C) and after completion of the freezing program (-80 degrees C, followed by storage under liquid nitrogen), motility and viability was well preserved only in the cells in DMSO-sucrose when subjected to a rapid cooling rate. Under these conditions, approximately 63% of spermatozoa were alive and showed progressive motility, Together, the average fertilization potential of cryopreserved semen was 58% (47% to 85%) compared with that of fresh semen. The procedure described here provides consistency and precision, and permits processing, freezing and storage of trout spermatozoa in less than 15 min.
publishDate 1996
dc.date.issued.none.fl_str_mv 1996
dc.date.accessioned.none.fl_str_mv 2017-04-27T18:52:41Z
2022-07-07T02:24:34Z
dc.date.available.none.fl_str_mv 2017-04-27T18:52:41Z
2022-07-07T02:24:34Z
dc.type.none.fl_str_mv Articulo
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.openaire.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.eissn.none.fl_str_mv 0
dc.identifier.folio.none.fl_str_mv D91I1140
D91I1140
dc.identifier.idwos.none.fl_str_mv WOS:A1996VH50000009
dc.identifier.isi.none.fl_str_mv WOS:A1996VH50000009
dc.identifier.issn.none.fl_str_mv 0044-8486
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10533/197734
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D91I1140
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url https://hdl.handle.net/10533/197734
dc.language.*.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv instname: Conicyt
reponame: Repositorio Digital RI2.0
instname: Conicyt
reponame: Repositorio Digital RI2.0
dc.relation.doi.none.fl_str_mv 10.1016/0044-8486(96)01275-6
dc.relation.projectid.none.fl_str_mv info:eu-repo/grantAgreement/Fondef/D91I1140
dc.relation.set.none.fl_str_mv info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relation.uri.none.fl_str_mv https://doi.org/10.1016/0044-8486(96)01275-6
dc.rights.driver.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.spatial.none.fl_str_mv AMSTERDAM
dc.publisher.none.fl_str_mv ELSEVIER SCIENCE BV
publisher.none.fl_str_mv ELSEVIER SCIENCE BV
repository.name.fl_str_mv Repositorio ANID
repository.mail.fl_str_mv aletelier@anid.cl
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