Evaluación del Efecto de un Tratamiento con Extracto de Calafate (Berberis Microphylla) Sobre un Modelo In Vivo de Resistencia a Insulina Inducida por Inflamación Ligada a Obesidad
ABSTRACT Obesity has turned into a world health burden, and Chile is not an exception. Obesity is a concerning illness not only because of its impact on daily life, but also because it is the root of several diseases. Metabolic dysfunctions are part of these diseases and among them, insulin resistan...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Chile |
| OAI Identifier: | oai:repositorio.anid.cl:10533/209646 |
| Acceso en línea: | https://hdl.handle.net/10533/209646 |
| Access Level: | acceso abierto |
| Palabra clave: | Medicina y Ciencias de la Salud Medicina Básica Farmacología y Farmacia |
| Sumario: | ABSTRACT Obesity has turned into a world health burden, and Chile is not an exception. Obesity is a concerning illness not only because of its impact on daily life, but also because it is the root of several diseases. Metabolic dysfunctions are part of these diseases and among them, insulin resistance has one of the higher prevalences. Adipose tissue inflammation has been described as a link between obesity and insulin resistance and evidences suggest that disrupting the inflammatory response elicited by obesity allows a recovery in insulin sensitivity. Hence, inflammatory response represents an interesting target in the search for agents with therapeutic potential. Natural compounds with therapeutic potential are of particular interest because of their almost absent secondary effects. This opens an opportunity for a Chilean Berry, the Calafate. Recent evidence suggest that due to its chemical composition, the Calafate acts as an anti-inflammatory agent. However, to determine if this anti-inflammatory activity can be projected to therapeutics requires an evaluation using an in vivo model. In this thesis the effect of a Calafate extract over insulin resistance prevention and/or treatment was evaluated using a model of obesity-induced insulin resistance. With this purpose, a polyphenol-rich Calfate extract was elaborated and characterized. Male C57BL/6J mice were feed with a cafeteria diet for a period of 12 or 14 weeks and together or during the last 4 weeks of treatment the calafate extract was administered in water at a total poliphenol dose of 50 mg/Kg/Day. Growth parameters, plasmatic and tissue analysis, inflammatory markers, glucose tolerance and insulin signaling pathway were evaluated. Our results showed that calafate extract administered during the last 4 weeks of cafeteria diet consumption has an anti-inflammatory and insulin-sensitizing effect in an in vivo obesity-induced insulin resistance model, suggesting a therapeutic potential for our polyphenol-rich calafate extract. |
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