Identification of Genes Associated with Testicular Cancer Aggressiveness

Introduction: Testicular germ cell tumors represent approximately 97% of testicular cancers. Histologically, they are classified into seminomas and non-seminomas, having diagnostic and prognostic applicability. Therapeutic success depends on early diagnosis associated with correct staging, the evalu...

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Detalhes bibliográficos
Autores: Medeiros, Graciele De Souza, Oliveira, Barbara Cardoso de, Barbosa Parula Fernandes , Vinicius, Cardoso, Vinicius Santos, Santos , Gabriel Arantes dos, Silva, Poliana Romão da, Reis , Sabrina Thalita dos
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Brasil
Recursos:Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA)
Repositorio:Revista Brasileira de Cancerologia (Online)
Idioma:portugués
inglés
español
OAI Identifier:oai:rbc.inca.gov.br:article/4553
Acesso em linha:https://rbc.inca.gov.br/index.php/revista/article/view/4553
Access Level:acceso abierto
Palavra-chave:Neoplasias Testiculares
Células Germinativas
Biologia Computacional/estatística & dados numéricos
Prognóstico
Testicular Neoplasms Prognosis, TCGA
Neoplasias Testiculares, PronósticoTCGA.
Descrição
Resumo:Introduction: Testicular germ cell tumors represent approximately 97% of testicular cancers. Histologically, they are classified into seminomas and non-seminomas, having diagnostic and prognostic applicability. Therapeutic success depends on early diagnosis associated with correct staging, the evaluation of biomarkers is important for the correct management of this disease. Objective: To identify genes that may be correlated with prognosis and survival in testicular cancer. Method: Bioinformatics analysis was performed using 137 testicular cancer samples from The Cancer Genome Atlas and 165 normal testicular tissue samples from The Genotype-Tissue Expression. Gene identification and subsequent analyzes were performed using GEPIA2. Results: Initially, in relation to gene expression, the 500 genes most significantly associated with overall survival from testicular cancer and the 500 with disease-free survival were evaluated. These two lists were then superimposed and a Venn diagram was constructed showing the 13 genes in common. Of these, only the protein-coding genes were kept, investigating which ones differed significantly from normal tissue in relation to gene expression. Only ATP10A, SAMD14 and PCAL4 showed a statistically significant difference, all of which were under-expressed in testicular cancer. The joint analysis of these genes was even more significant for overall and disease-free survival. Conclusion: Three genes were identified in the analysis in silico which demonstrated significative association of the expression with survival and prognosis of patients with testicular cancer.