Fine specificities of two lectins from Cymbosema roseum seeds: A lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide
The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), o...
| Autores: | , , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Brasil |
| Recursos: | Instituto Nacional de Pesquisas da Amazônia (INPA) |
| Repositorio: | Repositório Institucional do INPA |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio:1/16195 |
| Acesso em linha: | https://repositorio.inpa.gov.br/handle/1/16195 |
| Access Level: | acceso abierto |
| Palavra-chave: | Blood Group H Antigen Epitope Fucose Lectin Mannose Oligosaccharide Trisaccharide Chemical Structure Cymbosema Roseum Hemagglutination Legume Nonhuman Seed Plant Priority Journal Protein Binding Protein Purification Thermodynamics Abo Blood-group System Animal Chromatography, Affinity Erythrocytes Fabaceae Hemagglutination Inhibition Tests Mannose Mannose-binding Lectins Oligosaccharides Plant Lectins Polysaccharides Rabbits Seeds |
| Resumo: | The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fuc1,2Gal1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the (1,6) Man residue, the 3- and 4-hydroxyl group of the (1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewisx, Lewisy, Lewisa and Lewisb epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe. © 2011 The Author. |
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