Determination of carbamate pesticide in food using a biosensor based on reduced graphene oxide and acetylcholinesterase enzyme

Food safety is a major concern for human health and wellbeing all over the world. A novel and sensitive biosensor based on reduced graphene oxide (rGO) and the enzyme acetylcholinesterase (AChE) was developed and applied for the detection of carbaryl in food samples. The glassy carbon/rGO/AChE biose...

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Detalhes bibliográficos
Autores: da Silva, Martin K.L. [UNESP], Vanzela, Heloísa C. [UNESP], Defavari, Lucas M. [UNESP], Cesarino, Ivana [UNESP]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Brasil
Recursos:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/171462
Acesso em linha:http://dx.doi.org/10.1016/j.snb.2018.09.051
http://hdl.handle.net/11449/171462
Access Level:acceso abierto
Palavra-chave:Acetylcholinesterase enzyme
Biosensor
Carbaryl
Food safety
Reduced graphene oxide
Descrição
Resumo:Food safety is a major concern for human health and wellbeing all over the world. A novel and sensitive biosensor based on reduced graphene oxide (rGO) and the enzyme acetylcholinesterase (AChE) was developed and applied for the detection of carbaryl in food samples. The glassy carbon/rGO/AChE biosensor was characterized morphologically and electrochemically using scanning electron microscopy and cyclic voltammetry/electrochemical impedance spectroscopy, respectively. Optimum differential pulse voltammetry conditions led to a nanomolar detection limit, and determination of carbaryl in tomato was achieved.