Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa

BACKGROUND: Heat-shock protein A2 (HspA2) is correlated with sperm maturity, function and fertility, and a dysfunctional expression of such a gene results in abnormal spermatogenesis. the purpose of this study was to compare HspA2 gene expression in spermatozoa from oligozoospermic men and normozoos...

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Autores: Cedenho, Agnaldo Pereira [UNIFESP], Lima, Samira Barbosa [UNIFESP], Cenedeze, Marcos Antonio [UNIFESP], Spaine, Deborah Montagnini [UNIFESP], Ortiz, Valdemar [UNIFESP], Oehninger, S.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:Brasil
Institución:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/28989
Acceso en línea:http://dx.doi.org/10.1093/humrep/del055
http://repositorio.unifesp.br/handle/11600/28989
Access Level:acceso abierto
Palabra clave:gene expression
HspA2
male infertility
oligozoospermia
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oai_identifier_str oai:repositorio.unifesp.br:11600/28989
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repository_id_str
spelling Oligozoospermia and heat-shock protein expression in ejaculated spermatozoagene expressionHspA2male infertilityoligozoospermiaBACKGROUND: Heat-shock protein A2 (HspA2) is correlated with sperm maturity, function and fertility, and a dysfunctional expression of such a gene results in abnormal spermatogenesis. the purpose of this study was to compare HspA2 gene expression in spermatozoa from oligozoospermic men and normozoospermic controls. METHODS: Semen was obtained and analysed according to World Health Organization (World Health Organization, 1999) guidelines, morphology by Kruger's strict criteria. Seventeen patients with oligozoospermia and 21 fertile controls were studied. Total RNA was extracted from ejaculated and Percoll density-gradient-separated spermatozoa followed by semiquantitative RT-PCR analysis. the relative expression level of HspA2 was analysed according to the expression level of the housekeeping beta-actin gene. Serum hormonal profiles (FSH, LH and testosterone) and a peripheral karyotype were also performed. RESULTS: All patients possessed normal karyotype, and no significant hormonal differences were found between the two groups. the study group had significantly lower sperm concentration and normal morphology than the controls. Semiquantitative RT-PCR analysis of HspA2 showed significantly lower expression levels in the oligoteratozoospermic men when compared to controls (P = 0.0021). CONCLUSIONS: the HspA2 gene was down-regulated in sperm from infertile men with idiopathic oligoteratozoospermia, suggesting that such anomalies of gene expression might be associated with pathogenesis in some subtypes of male infertility.Universidade Federal de São Paulo, Div Urol, Dept Surg, São Paulo, BrazilUniversidade Federal de São Paulo, Div Urol, Div Nephrol, Dept Med, São Paulo, BrazilJones Inst Reprod Med, Norfolk, VA USAUniversidade Federal de São Paulo, Div Urol, Dept Surg, São Paulo, BrazilUniversidade Federal de São Paulo, Div Urol, Div Nephrol, Dept Med, São Paulo, BrazilWeb of ScienceOxford Univ PressUniversidade Federal de São Paulo (UNIFESP)Jones Inst Reprod Med2016-01-24T12:41:16Z2016-01-24T12:41:16Z2006-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1791-1794http://dx.doi.org/10.1093/humrep/del055Human Reproduction. Oxford: Oxford Univ Press, v. 21, n. 7, p. 1791-1794, 2006.10.1093/humrep/del0550268-1161http://repositorio.unifesp.br/handle/11600/28989WOS:000238907400021ark:/48912/001300002713sengHuman Reproductioninfo:eu-repo/semantics/openAccesshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPCedenho, Agnaldo Pereira [UNIFESP]Lima, Samira Barbosa [UNIFESP]Cenedeze, Marcos Antonio [UNIFESP]Spaine, Deborah Montagnini [UNIFESP]Ortiz, Valdemar [UNIFESP]Oehninger, S.2016-01-24T10:41:16Zoai:repositorio.unifesp.br:11600/28989Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T10:41:16Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa
title Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa
spellingShingle Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa
Cedenho, Agnaldo Pereira [UNIFESP]
gene expression
HspA2
male infertility
oligozoospermia
title_short Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa
title_full Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa
title_fullStr Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa
title_full_unstemmed Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa
title_sort Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa
dc.creator.none.fl_str_mv Cedenho, Agnaldo Pereira [UNIFESP]
Lima, Samira Barbosa [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Spaine, Deborah Montagnini [UNIFESP]
Ortiz, Valdemar [UNIFESP]
Oehninger, S.
author Cedenho, Agnaldo Pereira [UNIFESP]
author_facet Cedenho, Agnaldo Pereira [UNIFESP]
Lima, Samira Barbosa [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Spaine, Deborah Montagnini [UNIFESP]
Ortiz, Valdemar [UNIFESP]
Oehninger, S.
author_role author
author2 Lima, Samira Barbosa [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Spaine, Deborah Montagnini [UNIFESP]
Ortiz, Valdemar [UNIFESP]
Oehninger, S.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Jones Inst Reprod Med
dc.subject.por.fl_str_mv gene expression
HspA2
male infertility
oligozoospermia
topic gene expression
HspA2
male infertility
oligozoospermia
description BACKGROUND: Heat-shock protein A2 (HspA2) is correlated with sperm maturity, function and fertility, and a dysfunctional expression of such a gene results in abnormal spermatogenesis. the purpose of this study was to compare HspA2 gene expression in spermatozoa from oligozoospermic men and normozoospermic controls. METHODS: Semen was obtained and analysed according to World Health Organization (World Health Organization, 1999) guidelines, morphology by Kruger's strict criteria. Seventeen patients with oligozoospermia and 21 fertile controls were studied. Total RNA was extracted from ejaculated and Percoll density-gradient-separated spermatozoa followed by semiquantitative RT-PCR analysis. the relative expression level of HspA2 was analysed according to the expression level of the housekeeping beta-actin gene. Serum hormonal profiles (FSH, LH and testosterone) and a peripheral karyotype were also performed. RESULTS: All patients possessed normal karyotype, and no significant hormonal differences were found between the two groups. the study group had significantly lower sperm concentration and normal morphology than the controls. Semiquantitative RT-PCR analysis of HspA2 showed significantly lower expression levels in the oligoteratozoospermic men when compared to controls (P = 0.0021). CONCLUSIONS: the HspA2 gene was down-regulated in sperm from infertile men with idiopathic oligoteratozoospermia, suggesting that such anomalies of gene expression might be associated with pathogenesis in some subtypes of male infertility.
publishDate 2006
dc.date.none.fl_str_mv 2006-07-01
2016-01-24T12:41:16Z
2016-01-24T12:41:16Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/humrep/del055
Human Reproduction. Oxford: Oxford Univ Press, v. 21, n. 7, p. 1791-1794, 2006.
10.1093/humrep/del055
0268-1161
http://repositorio.unifesp.br/handle/11600/28989
WOS:000238907400021
dc.identifier.dark.fl_str_mv ark:/48912/001300002713s
url http://dx.doi.org/10.1093/humrep/del055
http://repositorio.unifesp.br/handle/11600/28989
identifier_str_mv Human Reproduction. Oxford: Oxford Univ Press, v. 21, n. 7, p. 1791-1794, 2006.
10.1093/humrep/del055
0268-1161
WOS:000238907400021
ark:/48912/001300002713s
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Human Reproduction
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.format.none.fl_str_mv 1791-1794
dc.publisher.none.fl_str_mv Oxford Univ Press
publisher.none.fl_str_mv Oxford Univ Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1853670547020316672
score 15.300719

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