Selection and classification of post bovine oocytes
In vitro embryo production (IVEP) has enabled advances in the genetic improvement of animal herds by promoting the use of the genetic material of a single female oocyte donor for in vitro fertilization (IVF), forming embryos that will be transferred to several recipients while maintaining the donor&...
| Authors: | , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2024 |
| Country: | Brasil |
| Institution: | Editora JRG |
| Repository: | Revista JRG de Estudos Acadêmicos |
| Language: | Portuguese |
| OAI Identifier: | oai:ojs2.revistajrg.com:article/1219 |
| Online Access: | http://revistajrg.com/index.php/jrg/article/view/1219 |
| Access Level: | Open access |
| Keyword: | Bovinos Embriões Ovócitos PIVE Cattle Embryos Oocytes |
| Summary: | In vitro embryo production (IVEP) has enabled advances in the genetic improvement of animal herds by promoting the use of the genetic material of a single female oocyte donor for in vitro fertilization (IVF), forming embryos that will be transferred to several recipients while maintaining the donor's genetics, which consequently reduces the time intervals between generations. There are a number of steps required to carry out IVP, such as follicular aspiration, oocyte classification, IVF and embryo transfer (ET). The aim of this study was to identify and classify viable oocytes to be matured and fertilized according to their quality grade, namely grade I, grade II, grade III, grade IV and grade V, where grade I shows higher quality gametes and grade V lower quality gametes. We used 70 ovaries obtained from a commercial slaughterhouse in the city of Santa Terezinha do Itaipu - PR, which were transported to the Clinical Analysis laboratory of the Centro Universitário Dinâmica das Cataratas, in the city of Foz do Iguaçu - PR. The ovaries were transported in a 0.9% saline solution at a temperature of 35 to 37°C and then aspirated to obtain the oocytes, which were then classified according to their quality. 278 oocytes were recovered and the largest number of grade V oocytes were found, accounting for 31.65% of the volume obtained, totaling 88 oocytes. In smaller quantities, 10.43% of the oocytes were classified as grade I, totaling 29 structures. The quality of the oocytes can vary according to various factors such as the age of the donors, breed and reproductive period, the transportation of the ovaries, the time from slaughter to collection and aspiration and the ability to aspirate the follicles. |
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