Micropropagação de Calendula officinalis L
Micropropagation of Calendula officinalis L. is usually propagated through seeds and therefore shows high diversity in flower size and colour, what causes quantitative and qualitative chemical variability. A micropropagation protocol was established for clonal propagation of this species to achieve...
| Autores: | , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2006 |
| País: | Brasil |
| Recursos: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.unesp.br:11449/68777 |
| Acesso em linha: | http://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo9_v8_n2.pdf http://hdl.handle.net/11449/68777 |
| Access Level: | acceso abierto |
| Palavra-chave: | Calêndula Medicinal plant Micropropagation kinetin biomass clonal variation controlled study culture medium flower harvesting marigold micropropagation Murashige and Skoog medium nonhuman phytotherapy plant root plant seed shoot growth species cultivation Calendula Calendula officinalis |
| Resumo: | Micropropagation of Calendula officinalis L. is usually propagated through seeds and therefore shows high diversity in flower size and colour, what causes quantitative and qualitative chemical variability. A micropropagation protocol was established for clonal propagation of this species to achieve homogeneous biomass, more appropriate for the production of phytotherapics. Explants harvested from capitula were the most appropriate for the micropropagation process. MS culture medium supplemented with 1.0 mgL-1 BAP and 6.0 gL-1 Phytagel™ enhanced shoot proliferation, while MS medium supplemented with 0.5 mgL-1 Kinetin and 6.0 gL-1 Phytagel™, increased shoot elongation. Plantlets (80%) cultured in MS/2 medium supplemented with 1.0 mgL-1 de IBA rooted. |
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