Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix

Despite the fact that coffee rust was first investigated scientifically more than a century ago, and that the disease is one of the major constraints to coffee production - constantly changing the socio-economic and historical landscape of the crop - critical aspects of the life cycle of the pathoge...

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Authors: Carvalho, Carlos Roberto, Fernandes, Ronaldo C., Carvalho, Guilherme Mendes Almeida, Barreto, Robert W., Evans, Harry C.
Format: article
Status:Published version
Publication Date:2011
Country:Brasil
Institution:Universidade Federal de Viçosa (UFV)
Repository:LOCUS Repositório Institucional da UFV
Language:English
OAI Identifier:oai:locus.ufv.br:123456789/12890
Online Access:https://doi.org/10.1371/journal.pone.0026387
http://www.locus.ufv.br/handle/123456789/12890
Access Level:Open access
Keyword:Cryptosexuality
Genetic Diversity
Hemileia vastatrix
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spelling Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrixCryptosexualityGenetic DiversityHemileia vastatrixDespite the fact that coffee rust was first investigated scientifically more than a century ago, and that the disease is one of the major constraints to coffee production - constantly changing the socio-economic and historical landscape of the crop - critical aspects of the life cycle of the pathogen, Hemileia vastatrix, remain unclear. The asexual urediniospores are regarded as the only functional propagule: theoretically, making H. vastatrix a clonal species. However, the well-documented emergence of new rust pathotypes and the breakdown in genetic resistance of coffee cultivars, present a paradox. Here, using computer-assisted DNA image cytometry, following a modified nuclear stoichiometric staining technique with Feulgen, we show that meiosis occurs within the urediniospores. Stages of spore development were categorised based on morphology, from the spore-mother cell through to the germinating spore, and the relative nuclear DNA content was quantified statistically at each stage. Hidden sexual reproduction disguised within the asexual spore (cryptosexuality) could explain why new physiological races have arisen so often and so quickly in Hemileia vastatrix. This could have considerable implications for coffee breeding strategies and may be a common event in rust fungi, especially in related genera occupying the same basal phylogenetic lineages.Plos One2017-11-08T13:08:44Z2017-11-08T13:08:44Z2011-11-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1932-6203https://doi.org/10.1371/journal.pone.0026387http://www.locus.ufv.br/handle/123456789/12890engv. 6, n. 11, e26387, Nov. 2011info:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVCarvalho, Carlos RobertoFernandes, Ronaldo C.Carvalho, Guilherme Mendes AlmeidaBarreto, Robert W.Evans, Harry C.2024-07-12T08:22:01Zoai:locus.ufv.br:123456789/12890Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T08:22:01LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix
title Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix
spellingShingle Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix
Carvalho, Carlos Roberto
Cryptosexuality
Genetic Diversity
Hemileia vastatrix
title_short Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix
title_full Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix
title_fullStr Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix
title_full_unstemmed Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix
title_sort Cryptosexuality and the genetic diversity paradox in coffee rust, Hemileia vastatrix
dc.creator.none.fl_str_mv Carvalho, Carlos Roberto
Fernandes, Ronaldo C.
Carvalho, Guilherme Mendes Almeida
Barreto, Robert W.
Evans, Harry C.
author Carvalho, Carlos Roberto
author_facet Carvalho, Carlos Roberto
Fernandes, Ronaldo C.
Carvalho, Guilherme Mendes Almeida
Barreto, Robert W.
Evans, Harry C.
author_role author
author2 Fernandes, Ronaldo C.
Carvalho, Guilherme Mendes Almeida
Barreto, Robert W.
Evans, Harry C.
author2_role author
author
author
author
dc.subject.por.fl_str_mv Cryptosexuality
Genetic Diversity
Hemileia vastatrix
topic Cryptosexuality
Genetic Diversity
Hemileia vastatrix
description Despite the fact that coffee rust was first investigated scientifically more than a century ago, and that the disease is one of the major constraints to coffee production - constantly changing the socio-economic and historical landscape of the crop - critical aspects of the life cycle of the pathogen, Hemileia vastatrix, remain unclear. The asexual urediniospores are regarded as the only functional propagule: theoretically, making H. vastatrix a clonal species. However, the well-documented emergence of new rust pathotypes and the breakdown in genetic resistance of coffee cultivars, present a paradox. Here, using computer-assisted DNA image cytometry, following a modified nuclear stoichiometric staining technique with Feulgen, we show that meiosis occurs within the urediniospores. Stages of spore development were categorised based on morphology, from the spore-mother cell through to the germinating spore, and the relative nuclear DNA content was quantified statistically at each stage. Hidden sexual reproduction disguised within the asexual spore (cryptosexuality) could explain why new physiological races have arisen so often and so quickly in Hemileia vastatrix. This could have considerable implications for coffee breeding strategies and may be a common event in rust fungi, especially in related genera occupying the same basal phylogenetic lineages.
publishDate 2011
dc.date.none.fl_str_mv 2011-11-05
2017-11-08T13:08:44Z
2017-11-08T13:08:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1932-6203
https://doi.org/10.1371/journal.pone.0026387
http://www.locus.ufv.br/handle/123456789/12890
identifier_str_mv 1932-6203
url https://doi.org/10.1371/journal.pone.0026387
http://www.locus.ufv.br/handle/123456789/12890
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 6, n. 11, e26387, Nov. 2011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
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institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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