Atividade imunoestimulatória e anticâncer de galactanas sulfatadas de Caulerpa cupressoides var. flabellata
Marine seaweeds are rich sources of compounds with pharmacological properties. Among these compounds, sulfated polysaccharides (SP) are known for their structural diversity, high content and because they present great potential of use in several human activities. For these reasons, studies with SP h...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | Brasil |
| Institución: | Universidade Federal do Rio Grande do Norte (UFRN) |
| Repositorio: | Repositório Institucional da UFRN |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.ufrn.br:123456789/27359 |
| Acceso en línea: | https://repositorio.ufrn.br/jspui/handle/123456789/27359 |
| Access Level: | acceso abierto |
| Palabra clave: | Polissacarídeos sulfatados Galactanas sulfatadas Atividade imunoestimulatória Mediadores inflamatórios Melanoma Migração celular CNPQ::CIENCIAS DA SAUDE |
| Sumario: | Marine seaweeds are rich sources of compounds with pharmacological properties. Among these compounds, sulfated polysaccharides (SP) are known for their structural diversity, high content and because they present great potential of use in several human activities. For these reasons, studies with SP have gained prominence in biomedical research. Thus, the aim of this work was to characterize structurally the SP from Caulerpa cupressoides var. flabellata and to evaluate its immunostimulatory and anticancer potential, in vitro. For this, the SP were extracted and characterized by nuclear magnetic resonance (NMR) spectroscopy. In addition, its immunostimulatory and anticancer effects were evaluated by quantification of different inflammatory mediators in RAW 264.7 murine macrophages, as well as their inhibitory effects on tumorigenic markers in murine melanoma B16-F10 cells, respectively. NMR analysis revealed that the polysaccharide fractions of C. cupressoides are rich in galactans, consisting of units of sulfated and pyruvated β-D-galactopyranoses. These SP had no cytotoxic effects on cell lines tested. However, they promoted significant increases in the production of inflammatory mediators: nitric oxide, reactive oxygen species and cytokines TNF-α and IL-6. With respect to anticancer activity, PS inhibited migration, melanin production and clonogenic formation in B16-F10 cells. Together, the results of this study showed that C. cupressoides sulfated galactans may play a potent immunostimulatory and anticancer effect, with potential use in new products with biomedical and biotechnological applications. However, additional studies should be performed to elucidate the cellular and molecular mechanisms involved in the observed responses, as well as to verify these effects in other study models. |
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