Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)

Peripheral arterial chemoreceptors monitor the levels of arterial blood gases and adjust ventilation and perfusion to meet metabolic demands. These chemoreceptors are present in all vertebrates studied to date but have not been described fully in reptiles other than turtles. The goals of this study...

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Autores: Reyes, Catalina, Fong, Angelina Y., Leite, Cleo A C, Abe, Augusto S. [UNESP], Milsom, William K.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/307545
Acceso en línea:http://dx.doi.org/10.1242/jeb.249222
https://hdl.handle.net/11449/307545
Access Level:acceso abierto
Palabra clave:Aortic chemoreceptors
Cardiac shunt
Carotid body
Chemoreceptors
Pulmonary chemoreceptors
Rattlesnake
Ventilation
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spelling Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)Aortic chemoreceptorsCardiac shuntCarotid bodyChemoreceptorsPulmonary chemoreceptorsRattlesnakeVentilationPeripheral arterial chemoreceptors monitor the levels of arterial blood gases and adjust ventilation and perfusion to meet metabolic demands. These chemoreceptors are present in all vertebrates studied to date but have not been described fully in reptiles other than turtles. The goals of this study were to (1) identify functional chemosensory areas in the South American rattlesnake (Crotalus durissus), (2) determine the neurochemical content of putative chemosensory cells in these areas and (3) determine the role each area plays in ventilatory and cardiovascular control. To this end, rattlesnakes were instrumented with transonic flow probes, arterial catheters and subcutaneous impedance electrodes to measure shunt fraction, heart rate, blood pressure and ventilation. The catheters were placed at three putative chemosensory sites, the bases of the aortic arch and pulmonary artery, and the carotid bifurcation, for site-specific activation with sodium cyanide (NaCN). These same sites were subsequently examined using immunohistochemical markers for acetylcholine, tyrosine hydroxylase (the rate-limiting enzyme in catecholamine synthesis) and serotonin to identify putative oxygen-sensing cells. All three sites were chemosensory and stimulating each led to cardiovascular (shunt fraction and heart rate) and respiratory adjustments although not in an identical fashion. All three chemosensory areas contained cells positive for serotonin; however, cells positive for vesicular acetylcholine transporter (VAChT) were found only in the aorta and pulmonary artery. We found no labelling for tyrosine hydroxylase at any site.Natural Sciences and Engineering Research Council of CanadaSchool of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USADepartment of Anatomy and Physiology, University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Physiological Sciences Federal University of São Carlos (UFSCar)Departamento de Zoologia Universidade Estadual PaulistaDepartment of Zoology University of British ColumbiaDepartamento de Zoologia Universidade Estadual PaulistaNatural Sciences and Engineering Research Council of Canada: GRO10285Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)University of British Columbia2025-04-29T20:09:47Z2025-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1242/jeb.249222The Journal of experimental biology, v. 228, n. 4, 2025.1477-9145https://hdl.handle.net/11449/30754510.1242/jeb.2492222-s2.0-85219157619Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengThe Journal of experimental biologyinfo:eu-repo/semantics/openAccessReyes, CatalinaFong, Angelina Y.Leite, Cleo A CAbe, Augusto S. [UNESP]Milsom, William K.2025-04-30T13:56:53Zoai:repositorio.unesp.br:11449/307545Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-04-30T13:56:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)
title Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)
spellingShingle Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)
Reyes, Catalina
Aortic chemoreceptors
Cardiac shunt
Carotid body
Chemoreceptors
Pulmonary chemoreceptors
Rattlesnake
Ventilation
title_short Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)
title_full Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)
title_fullStr Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)
title_full_unstemmed Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)
title_sort Distribution and role of peripheral arterial chemoreceptors in cardio-respiratory control of the South American rattlesnake (Crotalus durissus)
dc.creator.none.fl_str_mv Reyes, Catalina
Fong, Angelina Y.
Leite, Cleo A C
Abe, Augusto S. [UNESP]
Milsom, William K.
author Reyes, Catalina
author_facet Reyes, Catalina
Fong, Angelina Y.
Leite, Cleo A C
Abe, Augusto S. [UNESP]
Milsom, William K.
author_role author
author2 Fong, Angelina Y.
Leite, Cleo A C
Abe, Augusto S. [UNESP]
Milsom, William K.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (UNESP)
University of British Columbia
dc.subject.por.fl_str_mv Aortic chemoreceptors
Cardiac shunt
Carotid body
Chemoreceptors
Pulmonary chemoreceptors
Rattlesnake
Ventilation
topic Aortic chemoreceptors
Cardiac shunt
Carotid body
Chemoreceptors
Pulmonary chemoreceptors
Rattlesnake
Ventilation
description Peripheral arterial chemoreceptors monitor the levels of arterial blood gases and adjust ventilation and perfusion to meet metabolic demands. These chemoreceptors are present in all vertebrates studied to date but have not been described fully in reptiles other than turtles. The goals of this study were to (1) identify functional chemosensory areas in the South American rattlesnake (Crotalus durissus), (2) determine the neurochemical content of putative chemosensory cells in these areas and (3) determine the role each area plays in ventilatory and cardiovascular control. To this end, rattlesnakes were instrumented with transonic flow probes, arterial catheters and subcutaneous impedance electrodes to measure shunt fraction, heart rate, blood pressure and ventilation. The catheters were placed at three putative chemosensory sites, the bases of the aortic arch and pulmonary artery, and the carotid bifurcation, for site-specific activation with sodium cyanide (NaCN). These same sites were subsequently examined using immunohistochemical markers for acetylcholine, tyrosine hydroxylase (the rate-limiting enzyme in catecholamine synthesis) and serotonin to identify putative oxygen-sensing cells. All three sites were chemosensory and stimulating each led to cardiovascular (shunt fraction and heart rate) and respiratory adjustments although not in an identical fashion. All three chemosensory areas contained cells positive for serotonin; however, cells positive for vesicular acetylcholine transporter (VAChT) were found only in the aorta and pulmonary artery. We found no labelling for tyrosine hydroxylase at any site.
publishDate 2025
dc.date.none.fl_str_mv 2025-04-29T20:09:47Z
2025-02-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1242/jeb.249222
The Journal of experimental biology, v. 228, n. 4, 2025.
1477-9145
https://hdl.handle.net/11449/307545
10.1242/jeb.249222
2-s2.0-85219157619
url http://dx.doi.org/10.1242/jeb.249222
https://hdl.handle.net/11449/307545
identifier_str_mv The Journal of experimental biology, v. 228, n. 4, 2025.
1477-9145
10.1242/jeb.249222
2-s2.0-85219157619
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv The Journal of experimental biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1853671783773765632
score 15,136285

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