Erythropoietin in the Locus coeruleus attenuates the ventilatory response to CO2 in rats

The Locus coeruleus (LC) is a pontine area that contributes to the CO2/pH chemosensitivity. LC cells express erythropoietin (Epo) receptors (EpoR), and Epo in the brainstem is a potent normoxic and hypoxic respiratory stimulant. However, a recent study showed that the intra-cisternal injection (ICI)...

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Bibliographic Details
Authors: Silva, Carlos A. [UNESP], Vicente, Mariane C. [UNESP], Tenorio-Lopes, Luana, Soliz, Jorge, Gargaglioni, Luciane H. [UNESP]
Format: article
Status:Published version
Publication Date:2017
Country:Brasil
Institution:Universidade Estadual Paulista (UNESP)
Repository:Repositório Institucional da UNESP
Language:English
OAI Identifier:oai:repositorio.unesp.br:11449/162399
Online Access:http://dx.doi.org/10.1016/j.resp.2016.10.011
http://hdl.handle.net/11449/162399
Access Level:Open access
Keyword:Erythropoietin
Locus coeruleus
Central chemosensitivity
Hypercapnia
Ventilation
Description
Summary:The Locus coeruleus (LC) is a pontine area that contributes to the CO2/pH chemosensitivity. LC cells express erythropoietin (Epo) receptors (EpoR), and Epo in the brainstem is a potent normoxic and hypoxic respiratory stimulant. However, a recent study showed that the intra-cisternal injection (ICI) of Epo antagonist does not alter the hypercapnic ventilatory response in mice. As ICI leads to a widespread dispersal of the product throughout the brainstem, in this work we evaluated the specific impact of Epo in the LC-mediated ventilatory response to CO2 (by whole body plethysmography) in juvenile male Wistar rats. Normocapnic and hypercapnic ventilation were evaluated before and after unilateral microinjection of Epo (1 ng/100 nL) into the LC. To evaluate the long-term effect of Epo, the HcVR was re-evaluated 24h later. Our results show that Epo attenuates the hypercapnic ventilation. We conclude that Epo in the LC tunes the hypercapnia-induced hyperpnea. (C) 2016 Elsevier B.V. All rights reserved.