Cultivo primário de células hepáticas de Metynnis roosevelti (Pisces, Teleostei, Characidae)
In order to achieve monolayers and adhesion in cell cultures of hepatocytes from the subtropical fish Metynnis roosevelti (Pisces, Teleostei, Characidae) three different methodologies were used: enzyme cell dissociation by means of a system containing trypsin and versene 0.25%; by means of bovine fe...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2000 |
| País: | Brasil |
| Institución: | Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
| Repositorio: | Brazilian Journal of Veterinary Research and Animal Science |
| Idioma: | inglés |
| OAI Identifier: | oai:revistas.usp.br:article/5785 |
| Acceso en línea: | https://www.revistas.usp.br/bjvras/article/view/5785 |
| Access Level: | acceso abierto |
| Palabra clave: | Hepatócitos Cultura de célula Células Peixes Metynnis roosevelti Liver cells Cell culture Cells Pisces |
| Sumario: | In order to achieve monolayers and adhesion in cell cultures of hepatocytes from the subtropical fish Metynnis roosevelti (Pisces, Teleostei, Characidae) three different methodologies were used: enzyme cell dissociation by means of a system containing trypsin and versene 0.25%; by means of bovine fetal serum and by the use of blood serum obtained from adult specimens of M. roosevelti. The cell medium used has been a modified F10-199 by the addition of 2 mM L-glutamine, 10 µg/mL insulin, 50 µg/mL fibronectin; and antibiotics. The best results in terms of cell dissociation as well as in terms of adhesion and confluence of the cells monolayer has been achieved by means of the enzymatic cell dissociation. |
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