Chromosome analysis in saccodon wagneri (Characiformes) and insights into the karyotype evolution of parodontidae

Parodontidae is a relatively small group of Neotropical characiform fishes consisting of three genera (Apareiodon, Parodon, and Saccodon) with 32 valid species. A vast cytogenetic literature is available on Apareiodon and Parodon, but to date, there is no cytogenetic data about Saccodon, a genus tha...

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Detalles Bibliográficos
Autores: Nirchio, Mauro, Masache, Maria Cecilia, Paim, Fabilene Gomes [UNESP], Cioffi, Marcelo de Bello, Filho, Orlando Moreira, Barriga, Ramiro, Oliveira, Claudio [UNESP], Rossi, Anna Rita
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/206037
Acceso en línea:http://dx.doi.org/10.1590/1982-0224-2020-0103
http://hdl.handle.net/11449/206037
Access Level:acceso abierto
Palabra clave:Ag-NOR
Cytogenetics
FISH
Heterochromatin
ZW sex chromosomes
Descripción
Sumario:Parodontidae is a relatively small group of Neotropical characiform fishes consisting of three genera (Apareiodon, Parodon, and Saccodon) with 32 valid species. A vast cytogenetic literature is available on Apareiodon and Parodon, but to date, there is no cytogenetic data about Saccodon, a genus that contains only three species with a trans-Andean distribution. In the present study the karyotype of S. wagneri was described, based on both conventional (Giemsa staining, Ag-NOR, C-bands) and molecular (repetitive DNA mapping by fluorescent in situ hybridization) methods. A diploid chromosome number of 2n = 54 was observed in both sexes, and the presence of heteromorphic sex chromosomes of the ZZ/ ZW type was detected. The W chromosome has a terminal heterochromatin band that occupies approximately half of the long arm, being this band approximately half the size of the Z chromosome. The FISH assay showed a synteny of the 18S-rDNA and 5S-rDNA genes in the chromosome pair 14, and the absence of interstitial telomeric sites. Our data reinforce the hypothesis of a conservative karyotype structure in Parodontidae and suggest an ancient origin of the sex chromosomes in the fishes of this family.