Antiproliferative effect of apocynin in cervical epithelial cells infected by HPV 16 involves change of ROS production and cell cycle

The cervical cancer is characterized as a public health problem. The human papillomavirus, especially the high-risk types 16 and 18, is present in 99.7% of cases of invasive cervical cancers. Due to high prevalence, the search for new drugs for the treatment is continuous. The apocynin, compound bel...

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Detalles Bibliográficos
Autores: Lima, Natalia Alves, Scharf Santana, Natalia de Carvalho, Alves de Lima, Nayara Cristina, Lazarin-Bidoia, Danielle, Bonfim-Mendonca, Patricia de Souza, Nakamura, Tania Ueda, Nakamura, Celso Vataru, Lopes Consolaro, Marcia Edilaine, Ximenes, Valdecir Farias [UNESP], Silva, Sueli de Oliveira
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Brasil
Institución:Universidade Estadual Paulista (UNESP)
Repositorio:Repositório Institucional da UNESP
Idioma:inglés
OAI Identifier:oai:repositorio.unesp.br:11449/163428
Acceso en línea:http://dx.doi.org/10.1007/s00044-017-1984-9
http://hdl.handle.net/11449/163428
Access Level:acceso abierto
Palabra clave:SiHa
HPV 16
Apocynin
Reactive oxygen species
Descripción
Sumario:The cervical cancer is characterized as a public health problem. The human papillomavirus, especially the high-risk types 16 and 18, is present in 99.7% of cases of invasive cervical cancers. Due to high prevalence, the search for new drugs for the treatment is continuous. The apocynin, compound belonging to the class of catechol-methoxy acetophenone, has been used as an effective and non-toxic inhibitor of the NADPH oxidase complex, in many experimental models. Some early studies have indicated that apocynin also exerts antiproliferative activity inducing cell death by apoptosis. Thus, we evaluated the effect of apocynin in cervical epithelial cells infected by human papillomavirus 16 (SiHa cells) by analyzing oxygen species production, cell cycle, cell morphology, cell volume, membrane integrity and mitochondria membrane potential. Our data showed that apocynin induces antiproliferative effects by decreasing reactive oxygen species production and inducing cell cycle arrest, activating pathways that induce apoptosis cell death.

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