Lipase production by bacterial isolates from petroleum contaminated soil - DOI: 10.4025/actascitechnol.v32i1.7550
Lipase production by bacterial isolates from petroleum contaminated soil. This paper aims to present alternatives to the production of lipolytic enzymes from microorganisms isolated from soil with a history of contact with oil from the region of Sergipe, by submerged fermentation using palm oil as i...
| Authors: | , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2009 |
| Country: | Brasil |
| Institution: | Universidade Estadual de Maringá (UEM) |
| Repository: | Acta scientiarum. Technology (Online) |
| Language: | Portuguese |
| OAI Identifier: | oai:periodicos.uem.br/ojs:article/7550 |
| Online Access: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7550 |
| Access Level: | Open access |
| Keyword: | submerged fermentation lipolytic activities inducer fermentação submersa atividade lipolítica indutor |
| Summary: | Lipase production by bacterial isolates from petroleum contaminated soil. This paper aims to present alternatives to the production of lipolytic enzymes from microorganisms isolated from soil with a history of contact with oil from the region of Sergipe, by submerged fermentation using palm oil as inducer. The experiments were conduced in 250 mL Erlenmeyer flasks in a shaker (170 rpm), containing 125 mL (%, w v-1) of KH2PO4, MgSO4.7H2O, NaNO3, yeast extract, peptone and starch. The pH was 5.0 and 7.0; incubation temperature was 30 and 37°C, respectively; fermentation time was 24, 48, 72, 96h, 120 and 144h; and the inducer concentration was 3 and 4%, added after 72h of fermentation. The determination of lipolytic activity was examined at 37°C, in a shaker in thermostatic bath with rotation of 82 rpm, using gum arabic 7% (w v-1), olive oil and water. The reactions were conducted for 10 min with agitation and later stopped by the addition of an acetone:ethanol:water solution, where released fatty acids were titrated with a solution of KOH (0.04 N), using phenolphthalein as an indicator. The maximum activity was determined as 4369.75 U mL-1 pH 7.0 and a temperature of 37°C, with the percentage of the inducer oil palm to 4%. |
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