Effect of resistance training on the reduction of edema, nociception, and nerve regeneration in a sciatic nerve compression model

Aims: To analyze the effects of ladder-climbing resistance training exercise on edema, nociception, and regeneration of the sciatic nerve in Wistar rats subjected to sciatic nerve compression. Methods: Twenty-four Wistar rats were divided into four groups: Control Group, Exercise Group, Injury Group...

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Detalles Bibliográficos
Autores: Antunes, Juliana Sobral, Lovison, Keli, Karvat, Jhenifer, Vieira, Lisyana, Peretti, Ana Luiza, Higuchi, Guilherme Hideaki, Bonfleur, Maria Lúcia, Brancalhão, Rose Meire Costa, Ribeiro, Lucinéia de Fátima Chasko, Bertolini, Gladson Ricardo Flor
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Brasil
Institución:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
Repositorio:Scientia Medica (Porto Alegre. Online)
Idioma:portugués
OAI Identifier:oai:ojs.revistaseletronicas.pucrs.br:article/23711
Acceso en línea:https://revistaseletronicas.pucrs.br/scientiamedica/article/view/23711
Access Level:acceso abierto
Palabra clave:motor activity
sciatic nerve
sciatic neuropathy
pain measurement
nerve regeneration.
atividade motora
nervo isquiático
neuropatia ciática
medição da dor
regeneração nervosa.
Descripción
Sumario:Aims: To analyze the effects of ladder-climbing resistance training exercise on edema, nociception, and regeneration of the sciatic nerve in Wistar rats subjected to sciatic nerve compression. Methods: Twenty-four Wistar rats were divided into four groups: Control Group, Exercise Group, Injury Group, and Treated Group (injury and exercise). Injury Group and Treated Group were subjected to sciatic nerve compression with a hemostat for 30 seconds. On the third day after injury, Exercise Group and Treated Group began treatment with ladder-climbing resistance exercise. The treatment consisted in performing two series of 10 ladder climbs with a 100-gram overload and a one-minute interval between the series. The study was conducted for 22 days, during which time the animals were evaluated for edema and nociception. Twenty-two days after surgery, the animals were anesthetized for removal of a sciatic nerve fragment and analysis of the number of axons and fiber density. Thereafter, still under anesthesia, the animals were euthanized. Nerve sampling followed the routine histological processing protocol. Expressions of Neural Growth Factor and Brain-derived Neurotrophic Factor were evaluated by Western blotting. Results: There was no significant difference in edema size between groups. Control Group showed the highest nociceptive threshold compared to the other groups. The morphometric analysis showed no significant difference in number of axons and fiber density between groups. The expression of Brain-derived Neurotrophic Factor was greater in the Injury Group and the Treated Group compared to the Control Group. Conclusions: The proposed ladder-climbing resistance training was not effective in reducing edema and nociception or in increasing the number of axons and fiber density after sciatic nerve injury.