Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey

Maeda, Isabel Cabral [UNESP]; Sampaio, Aryele Nunes Da Cruz Encide [UNESP]; Flores Caron, Evelyn Fernanda [UNESP]; Nardy, José Fernando [UNESP]; Oliveira, Sarah Cristina De [UNESP]; Pereira, Juliano Gonçalves [UNESP]; Martins, Otávio Augusto [UNESP]

Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey

The determination of hydroxymethylfurfural (HMF) in bee honey is performed by the spectrophotometric methods Winkler's and White's. The Winkler method reads the absorbances in the visible region of the spectrum, and the White method measures in the ultraviolet (UV) and visible regions of t...

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Detalles Bibliográficos
Autores:	Maeda, Isabel Cabral [UNESP], Sampaio, Aryele Nunes Da Cruz Encide [UNESP], Flores Caron, Evelyn Fernanda [UNESP], Nardy, José Fernando [UNESP], Oliveira, Sarah Cristina De [UNESP], Pereira, Juliano Gonçalves [UNESP], Martins, Otávio Augusto [UNESP]
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2023
País:	Brasil
Institución:	Universidade Estadual Paulista (UNESP)
Repositorio:	Repositório Institucional da UNESP
Idioma:	inglés
OAI Identifier:	oai:repositorio.unesp.br:11449/244866
Acceso en línea:	http://dx.doi.org/10.1590/1981-6723.13322 http://hdl.handle.net/11449/244866
Access Level:	acceso abierto
Palabra clave:	Absorbance Bees Comparison HMF Physicochemical Spectrophotometer

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spelling	Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honeyAbsorbanceBeesComparisonHMFPhysicochemicalSpectrophotometerThe determination of hydroxymethylfurfural (HMF) in bee honey is performed by the spectrophotometric methods Winkler's and White's. The Winkler method reads the absorbances in the visible region of the spectrum, and the White method measures in the ultraviolet (UV) and visible regions of the spectrum. This study aims to compare the spectrophotometric methodologies of White and Winkler to the determination of HMF in bee honey. For the study, it was used a UV-visible spectrophotometer, 10 mm optical path quartz cuvettes, and ten bee honey samples of different flowering. Absorbances at 550 nm were analyzed by the Winkler method; absorbances at 284 nm and 336 nm in the White method; and a comparison of HMF concentration (mg/kg) was carried out between the methods. The results showed significant differences (p<0.05) in HMF concentration determined by both methods. The main conclusion is that the Winkler method presents higher HMF values than the White method.There are no significant differences in the absorbance readings in the evaluated methodsHMF values were different between the White and Winkler methodsThe Winkler method presents higher HMF values than the White methodUniversidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista, Faculdade de Medicina Veterinária e ZootecniaInstituto de Tecnologia de Alimentos - ITALUniversidade Estadual Paulista (UNESP)2023-07-29T11:14:56Z2023-07-29T11:14:56Z2023-05-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1981-6723.13322Brazilian Journal of Food Technology. Instituto de Tecnologia de Alimentos - ITAL, v. 26, p. -, 2023.1981-6723http://hdl.handle.net/11449/24486610.1590/1981-6723.13322S1981-67232023000100415S1981-67232023000100415.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Food Technologyinfo:eu-repo/semantics/openAccessMaeda, Isabel Cabral [UNESP]Sampaio, Aryele Nunes Da Cruz Encide [UNESP]Flores Caron, Evelyn Fernanda [UNESP]Nardy, José Fernando [UNESP]Oliveira, Sarah Cristina De [UNESP]Pereira, Juliano Gonçalves [UNESP]Martins, Otávio Augusto [UNESP]2025-10-22T16:55:20Zoai:repositorio.unesp.br:11449/244866Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462025-10-22T16:55:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv	Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey
title	Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey
spellingShingle	Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey Maeda, Isabel Cabral [UNESP] Absorbance Bees Comparison HMF Physicochemical Spectrophotometer
title_short	Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey
title_full	Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey
title_fullStr	Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey
title_full_unstemmed	Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey
title_sort	Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey
dc.creator.none.fl_str_mv	Maeda, Isabel Cabral [UNESP] Sampaio, Aryele Nunes Da Cruz Encide [UNESP] Flores Caron, Evelyn Fernanda [UNESP] Nardy, José Fernando [UNESP] Oliveira, Sarah Cristina De [UNESP] Pereira, Juliano Gonçalves [UNESP] Martins, Otávio Augusto [UNESP]
author	Maeda, Isabel Cabral [UNESP]
author_facet	Maeda, Isabel Cabral [UNESP] Sampaio, Aryele Nunes Da Cruz Encide [UNESP] Flores Caron, Evelyn Fernanda [UNESP] Nardy, José Fernando [UNESP] Oliveira, Sarah Cristina De [UNESP] Pereira, Juliano Gonçalves [UNESP] Martins, Otávio Augusto [UNESP]
author_role	author
author2	Sampaio, Aryele Nunes Da Cruz Encide [UNESP] Flores Caron, Evelyn Fernanda [UNESP] Nardy, José Fernando [UNESP] Oliveira, Sarah Cristina De [UNESP] Pereira, Juliano Gonçalves [UNESP] Martins, Otávio Augusto [UNESP]
author2_role	author author author author author author
dc.contributor.none.fl_str_mv	Universidade Estadual Paulista (UNESP)
dc.subject.por.fl_str_mv	Absorbance Bees Comparison HMF Physicochemical Spectrophotometer
topic	Absorbance Bees Comparison HMF Physicochemical Spectrophotometer
description	The determination of hydroxymethylfurfural (HMF) in bee honey is performed by the spectrophotometric methods Winkler's and White's. The Winkler method reads the absorbances in the visible region of the spectrum, and the White method measures in the ultraviolet (UV) and visible regions of the spectrum. This study aims to compare the spectrophotometric methodologies of White and Winkler to the determination of HMF in bee honey. For the study, it was used a UV-visible spectrophotometer, 10 mm optical path quartz cuvettes, and ten bee honey samples of different flowering. Absorbances at 550 nm were analyzed by the Winkler method; absorbances at 284 nm and 336 nm in the White method; and a comparison of HMF concentration (mg/kg) was carried out between the methods. The results showed significant differences (p<0.05) in HMF concentration determined by both methods. The main conclusion is that the Winkler method presents higher HMF values than the White method.
publishDate	2023
dc.date.none.fl_str_mv	2023-07-29T11:14:56Z 2023-07-29T11:14:56Z 2023-05-29
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	http://dx.doi.org/10.1590/1981-6723.13322 Brazilian Journal of Food Technology. Instituto de Tecnologia de Alimentos - ITAL, v. 26, p. -, 2023. 1981-6723 http://hdl.handle.net/11449/244866 10.1590/1981-6723.13322 S1981-67232023000100415 S1981-67232023000100415.pdf
url	http://dx.doi.org/10.1590/1981-6723.13322 http://hdl.handle.net/11449/244866
identifier_str_mv	Brazilian Journal of Food Technology. Instituto de Tecnologia de Alimentos - ITAL, v. 26, p. -, 2023. 1981-6723 10.1590/1981-6723.13322 S1981-67232023000100415 S1981-67232023000100415.pdf
dc.language.iso.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	Brazilian Journal of Food Technology
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	- application/pdf
dc.publisher.none.fl_str_mv	Instituto de Tecnologia de Alimentos - ITAL
publisher.none.fl_str_mv	Instituto de Tecnologia de Alimentos - ITAL
dc.source.none.fl_str_mv	SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP
instname_str	Universidade Estadual Paulista (UNESP)
instacron_str	UNESP
institution	UNESP
reponame_str	Repositório Institucional da UNESP
collection	Repositório Institucional da UNESP
repository.name.fl_str_mv	Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv	repositoriounesp@unesp.br
_version_	1853672227745038336
score	15,301629

Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey

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