Análise fitoquímica e estudo das atividades antimicrobiana, antioxidante e de inibição da enzima acetilcolinesterase das espécies Zanthoxylum rhoifolium e Zanthoxylum hyemale
The present study was designed to evaluate the biological activity from the stem bark of Zanthoxylum rhoifolium and Zanthoxylum hyemale. The neutral methanol extract and fractions of this extract, the basic fractions that were obtained after acid-basic extraction from the methanolic extract, and pur...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | Brasil |
| Institución: | Universidade Federal de Santa Maria (UFSM) |
| Repositorio: | Manancial - Repositório Digital da UFSM |
| Idioma: | portugués |
| OAI Identifier: | oai:repositorio.ufsm.br:1/10472 |
| Acceso en línea: | http://repositorio.ufsm.br/handle/1/10472 |
| Access Level: | acceso abierto |
| Palabra clave: | Zanthoxylum rhoifolium Zanthoxylum hyemale Alcalóides furoquinolínicos e benzofenantridínicos Atividades biológicas Atividade antioxidante Furoquinoline and benzophenanthridine alkaloids Biological activities Antioxidant potential CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
| Sumario: | The present study was designed to evaluate the biological activity from the stem bark of Zanthoxylum rhoifolium and Zanthoxylum hyemale. The neutral methanol extract and fractions of this extract, the basic fractions that were obtained after acid-basic extraction from the methanolic extract, and pure compounds isolated from these fraction, were investigated in vitro for antimicrobial activity, antioxidant potential and for acetylcholinesterase inhibition. From the dichlorometane fraction of Zanthoxylum rhoifolium were isolated three furoquinoline alkaloids: skimianine, 8-hidroxy-4,7-dimetoxy-furoquinoline, and γ-fagarin, four benzophenantridine alkaloids: dihydrocheleritrine, dihydroavicine, zanthoxyline and cheleritrine. From the hexane fraction, the lignanes sesamine and lupeol were also isolated. From the neutral methanol extract were isolates de aporfinic alkaloid magnoflorine. The minimal inhibitory concentration of cheleritrine (MIC =1.50 μg/ml) for all tested bacteria, and between 3.12 and 12.5 μg/ml for the tested yeasts, showed this alkaloid have a excellent antimicrobial activity compared to chloramphenicol (between 3.5 to 6.25 μg/m for bacteria) and nistatin (between 5.5 to 10.3 μg/ml for yeasts). The study highlighted the antimicrobial activity of Z. rhoifolium stem bark. This activity is probably due their higher benzophenantridinic alkaloids level. The furoquinoline alkaloids, despite their insignificant antimicrobial activity, were the most active against the enzime AChE. In addition, in this work, the amide tembamide was isolated from the steam bark of Zanthoxylum hyemale, and their derivatives used for a structure-ativity analysis. |
|---|