Avaliação da técnica de coloração AgNOR em células saudáveis de testículo de ovinos
The silver staining technique for AgNOR nucleolar organizer regions (NORs) is characterized by marking proteins linked to the ribosomal ribonucleic acid, evaluating cell proliferation. The aim was to study the validity of the AgNOR staining technique in the testicular cell proliferation in crossbred...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | Brasil |
| Institución: | Universidade do Oeste Paulista (UNOESTE) |
| Repositorio: | Biblioteca Digital de Teses e Dissertações da UNOESTE |
| Idioma: | portugués |
| OAI Identifier: | oai:bdtd.unoeste.br:tede/674 |
| Acceso en línea: | http://bdtd.unoeste.br:8080/tede/handle/tede/674 |
| Access Level: | acceso abierto |
| Palabra clave: | Ovinos Testículo Proliferação de células Técnica AgNOR, Matadouros Sheep Testis Cell Proliferation NOR, slaughterhouse Abattoirs CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| Sumario: | The silver staining technique for AgNOR nucleolar organizer regions (NORs) is characterized by marking proteins linked to the ribosomal ribonucleic acid, evaluating cell proliferation. The aim was to study the validity of the AgNOR staining technique in the testicular cell proliferation in crossbred ovine. Was used treis pairs forty crossbred sheep testes between six and ten months old.Wilcoxon and Spearman tests were used with a significance level of 5%. The mean NORs count in cells of the gonads right and left were respectively: spermatogonia (8.77±1.14 and 9.04±0.96), spermatocyte (4.99±2.00 and 6.20±2.07, P<0.05), Leydig (8.05±2.82 and 7.89±2.29) and Sertoli cells (8.07±1.88 and 7.61±2.16; P<0.05). There was a correlation between the mean values for right and left sides for the number of NORs (P<0.05) between Leydig x spermatocytes (0.49); spermatocytes x Sertoli (0.49) and Sertoli x Leydig (0.96). The study demonstrates that the AgNOR staining technique is indicated to evaluate the cell proliferative potential in ovine testis with practical implementation and low cost. |
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