Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae

Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.

Detalles Bibliográficos
Autores: Helvecio, Elisama, Rezende, Antonio Mauro, Bezerra, Maria Jose Ribeiro, De-Melo-Neto, Osvaldo Pompílio, Santos, Maria Alice Varjal Melo, Romão, Tatiany Patrícia, Ayres, Constância Flávia Junqueira
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Brasil
Institución:Fundação Oswaldo Cruz (FIOCRUZ)
Repositorio:Repositório Institucional da FIOCRUZ (ARCA)
Idioma:inglés
OAI Identifier:oai:arca.fiocruz.br:icict/67731
Acceso en línea:https://arca.fiocruz.br/handle/icict/67731
Access Level:acceso abierto
Palabra clave:Aedes aegypti
Temephos
Transcriptome
Metabolic resistance
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dc.title.none.fl_str_mv Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae
title Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae
spellingShingle Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae
Helvecio, Elisama
Aedes aegypti
Aedes aegypti
Temephos
Transcriptome
Metabolic resistance
title_short Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae
title_full Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae
title_fullStr Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae
title_full_unstemmed Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae
title_sort Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae
dc.creator.none.fl_str_mv Helvecio, Elisama
Rezende, Antonio Mauro
Bezerra, Maria Jose Ribeiro
De-Melo-Neto, Osvaldo Pompílio
Santos, Maria Alice Varjal Melo
Romão, Tatiany Patrícia
Ayres, Constância Flávia Junqueira
author Helvecio, Elisama
author_facet Helvecio, Elisama
Rezende, Antonio Mauro
Bezerra, Maria Jose Ribeiro
De-Melo-Neto, Osvaldo Pompílio
Santos, Maria Alice Varjal Melo
Romão, Tatiany Patrícia
Ayres, Constância Flávia Junqueira
author_role author
author2 Rezende, Antonio Mauro
Bezerra, Maria Jose Ribeiro
De-Melo-Neto, Osvaldo Pompílio
Santos, Maria Alice Varjal Melo
Romão, Tatiany Patrícia
Ayres, Constância Flávia Junqueira
author2_role author
author
author
author
author
author
dc.subject.other.none.fl_str_mv Aedes aegypti
topic Aedes aegypti
Aedes aegypti
Temephos
Transcriptome
Metabolic resistance
dc.subject.en.none.fl_str_mv Aedes aegypti
Temephos
Transcriptome
Metabolic resistance
description Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
publishDate 2024
dc.date.accessioned.fl_str_mv 2024-12-20T13:50:52Z
dc.date.available.fl_str_mv 2024-12-20T13:50:52Z
dc.date.issued.fl_str_mv 2024
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HELVECIO, Elisama et al. Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae. Researche Square, 2024.
dc.identifier.uri.fl_str_mv https://arca.fiocruz.br/handle/icict/67731
dc.identifier.doi.none.fl_str_mv 10.21203/rs.3.rs-4857258/v1
identifier_str_mv HELVECIO, Elisama et al. Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae. Researche Square, 2024.
10.21203/rs.3.rs-4857258/v1
url https://arca.fiocruz.br/handle/icict/67731
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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spelling 2024-12-20T13:50:52Z2024-12-20T13:50:52Z2024HELVECIO, Elisama et al. Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae. Researche Square, 2024.https://arca.fiocruz.br/handle/icict/6773110.21203/rs.3.rs-4857258/v1engSpringer NatureAedes aegyptiAedes aegyptiTemephosTranscriptomeMetabolic resistanceCharacterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Background: Resistance to organophosphate compounds is a serious concern in dealing with the control of mosquito vectors. Understanding the genetic and molecular basis of resistance is important not only to create strategies aimed at detecting and monitoring resistance in the field, but also to implement efficient control measures and support the development of new insecticides. Despite the extensive literature on insecticide resistance, the molecular basis of metabolic resistance is still poorly understood. Methods: In order to better understand the mechanisms of Aedes aegypti resistance to temephos, we performed NGS sequencing of RNA from the midgut tissue of Aedes aegypti larvae from temephos resistant (RecR) and susceptible (RecL) colonies. Bioinformatics analyses were then performed to assess the biological functions of differentially expressed genes, and the sequencing data were validated by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). Results: The transcriptome analysis mapped 6.084 genes, of which 202 were considered upregulated in RecR, with genes representing many detoxification enzyme families, including cytochrome-P450 oxidative enzymes, glutathione-S-transferases and glucosyl transferases. Other upregulated genes were mainly involved in the cuticle, carbohydrates, and lipid biosynthesis. For the downregulated profiles, we found 106 downregulated genes in the RecR colony, with molecules involved in protein synthesis, immunity and apoptosis process. Furthermore, we observed an enrichment of KEGG metabolic pathways related to resistance mechanisms. The results found in RT-qPCR confirm the findings of the transcriptome data. Conclusions: In this study, we investigated the changes in the transcriptome levels in an Ae. aegypti colony resistant to temephos. Our dataset showed that metabolic resistance mediated by multiple families of detoxifying enzymes is strongly associated with temephos metabolism, but other mechanisms may also contribute to temephos resistance in Ae. aegypti RecR colony.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZHelvecio, ElisamaRezende, Antonio MauroBezerra, Maria Jose RibeiroDe-Melo-Neto, Osvaldo PompílioSantos, Maria Alice Varjal MeloRomão, Tatiany PatríciaAyres, Constância Flávia JunqueiraLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/1aa9d1a3-50e7-445a-acf1-67b34166290b/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALCharacterization of the transcriptional cellular response.pdfCharacterization of the transcriptional cellular response.pdfapplication/pdf2266111https://arca.fiocruz.br/bitstreams/b504b407-e2a6-42b0-a437-accc8f054d14/downloadb6cf06876d2952a789db62062bb205a9MD52trueAnonymousREADTEXTCharacterization of the transcriptional cellular response.pdf.txtCharacterization of the transcriptional cellular response.pdf.txtExtracted texttext/plain63350https://arca.fiocruz.br/bitstreams/455a88eb-3412-4860-930f-d47faac29d72/downloadb361efd7f6b6e7ceeffba30e31eec0c5MD57falseAnonymousREADTHUMBNAILCharacterization of the transcriptional cellular response.pdf.jpgCharacterization of the transcriptional cellular response.pdf.jpgGenerated Thumbnailimage/jpeg29228https://arca.fiocruz.br/bitstreams/77ad8333-b186-4724-aeb6-73a4106ebf9a/downloadf5235ea7aa430cba178980f3d376a96dMD58falseAnonymousREADicict/677312025-12-11 08:32:41.296open.accessoai:arca.fiocruz.br:icict/67731https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-12-11T11:32:41Repositório Institucional da FIOCRUZ (ARCA) - 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